Efficiency of peripheral nerve gaps restoration by different types of tissue engineering constructs according to electromyography: experimental study

Tsymbalyuk, V. І.; Petriv, Taras; Medvediev, Volodymyr; Tatarchuk, M.M.; Draguntsova, N. G.; Vasyliev, Roman

doi:10.25305/unj.113909

Cited by 2 publications

(1 citation statement)

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“…Currently, the effectiveness of SN regeneration after transplantation of matrices based on collagen and neural stem cells polyglycolic acid and Schwann cells was shown [7]. More than 10 years ago, it was shown that bone marrow MSC can be differentiated into Schwann cells and thus support the regeneration of the peripheral nerve [8].…”

Section: Resultsmentioning

confidence: 99%

Efficacy of Fibrin Matrix With Neuroinduced Mesenchymal Stem Cells Transplantation for Restoration Sciatic Nerve Function After Its Complete Rupture in Rats

Pyatykop

Kaliuzhka²,

Shchegelska³

et al. 2019

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Peripheral nerves damage is a frequent pathology with significant socio-economic significance. The aim is to study the possibility of using fibrin matrices filled with neuroinduced mesenchymal stem cells (nMSC) to restore integrity of peripheral nerves. Methods. The study was carried out on 40 mongrel female rats. Sciatic nerves (SN) of all rats were intersected and then reconstituted using various methods. nMSC were obtained from ratsb one marrow and cultivated by special method. Results. Total anatomical rupture of SN without treatment led to persistent neurologic deficit (SFI = -98) in E1 group. Partial restoration of SN function increased to SFI = -37 on the 30 th day in E2 (operative reconstruction) group. Partial restoration of SN function occurred after 20 days (SFI = -64) in E3 group (transplanted acellular fibrin matrix). Partial restoration of SN function started at the 3 rd day, stably increased to SFI=-27 on 30 th day in E4 group (transplanted fibrin matrix with nMSC). Histological evaluation showed: there were alternating portions of connective tissue with portions of nerve fibers in E2 group; in E3 group large scar was formed at the place of transplanted fibrin matrix; in E4 were found spindle-shaped and stellate cells with long processes running from one side of SN to another, cells of connective tissue and thin nerve fibers. Conclusions. It has been shown that transplantation of the fibrin matrix with nMSC was more effective for treatment of SN trauma than transplantation of cell-free fibrin matrix and close to the results of surgical reconstruction.

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Section: Resultsmentioning

confidence: 99%