Efficiency of MS2 phage and Qβ phage removal by membrane filtration in water treatment: Applicability of real-time RT-PCR method

Langlet, Jérémie; Ogorzaly, Leslie; Schrotter, Jean‐Christophe; Machinal, Claire; Gaboriaud, Fabien; Duval, Jérôme F. L.; Gantzer, Christophe

doi:10.1016/j.memsci.2008.09.044

Cited by 85 publications

(43 citation statements)

References 21 publications

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“…HERATH et al (1999) ont ainsi montré que l'élimination des coliphages par microfiltration était liée à leurs charges électrostatiques et leurs points isoélectriques. Ce processus dépend donc des propriétés physico-chimiques des microorganismes et des adsorbants (BUSSCHER et al, 2008;LANGLET et al, 2009;RIzzO et al, 2008). Par ailleurs, les procédés de traitement par exclusion stérique seuls (filtration) peuvent s'avérer peu efficaces dans la rétention des virus en raison de leur faible taille, comprise entre 100 et 20 nm environ (FIKSDAL et LEIKNES, 2006).…”

Section: La Diversité Des Espèces Microbiennes Dans L'eau Potableunclassified

Le réseau de distribution d’eau potable : un écosystème complexe lié à des enjeux de santé publique

Poitelon

Joyeux

Welté

et al. 2012

rseau

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L’émergence de pathogènes dans l’eau destinée à la consommation humaine représente une préoccupation majeure en matière de santé publique pour les industriels et les pouvoirs publics concernés. Parmi ces pathogènes, certains sont d’origine fécale (Cryptosporidium, Campylobacter ou bien les rotavirus), alors que d’autres vivent dans l’environnent naturel (Legionella, Pseudomonas, Aeromonas ou bien les mycobactéries). Dans l’optique de mettre en place une analyse des risques liés à la présence de ces pathogènes, il est important d’accroître nos connaissances sur l’écologie de ces microorganismes et de développer des outils d’analyse afin de réaliser une meilleure surveillance sanitaire. Par conséquent, l’écologie microbienne du réseau de distribution d’eau potable doit être étudiée en détail, particulièrement vis-à-vis des propriétés physiologiques et la diversité des espèces microbiennes présentes, afin de mieux comprendre les interactions entre les espèces communément rencontrées et celles pathogènes.The emergence of pathogens in water intended for human consumption is a major concern in terms of the public health industry and the public authorities concerned. Among these pathogens, some are of faecal origin (Cryptosporidium, Campylobacter, or rotavirus), while others live in the natural environment (Legionella, Pseudomonas, Aeromonas or mycobacteria). In order to establish a risk analysis related to the presence of these pathogens, it is important to increase our knowledge on the ecology of these microorganisms and to develop analytical tools to achieve better health monitoring. Therefore, the microbial ecology of drinking water distribution networks must be studied in detail, especially with respect to the properties and physiological diversity of the microbial species present, in order to better understand the interactions between species commonly encountered and those that are pathogens

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Section: La Diversité Des Espèces Microbiennes Dans L'eau Potableunclassified

Le réseau de distribution d’eau potable : un écosystème complexe lié à des enjeux de santé publique

Poitelon

Joyeux

Welté

et al. 2012

rseau

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“…Overall the application of these technologies faces some limitations that include their inability to remove ionic species and nano-sized particles such as viruses. water-borne microorganisms to remove (Langlet et al, 2009). The virion of most enteric viruses consists of nucleic acid genome encapsulated by a capsid composed of proteins containing weak acid and base groups (e.g., carboxyl, sulfhydril and amine groups) that are ionizable (Brown and Sobsey, 2009).…”

Section: Membrane Technology For Water Treatmentmentioning

confidence: 99%

“…A low isoelectric point indicates a high net negative charge on the virus surface at typical pH values of natural water. Langlet et al, (2009) discussed the physicochemical characteristics of MS2 phage, identifYing it as a worst-case scenario for the evaluation of virus removal by membrane filtration (i.e., (i) small size, (ii) high negative surface charge and (iii) high degree of hydrophobicity).…”

Section: Virus Analysismentioning

confidence: 99%

Addition of a magnetite layer onto a polysulfone water treatment membrane to enhance virus removal

Raciny

Zodrow

et al. 2011

Water Science and Technology

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Addition of a Magnetite Layer onto a Polysulfone Water Treatment Membrane to Enhance Virus Removal by Isabel RacinyThe applicability of low-pressure membranes systems in distributed (point of use) water treatment is hindered by, among other things, their inability to remove potentially harmful viruses and ions via size exclusion.According to the USEPA and the Safe Drinking Water Act, drinking water treatment processes must be designed for 4-log virus removal. Batch experiments using magnetite nanoparticle (nano-Fe304) suspensions and water filtration experiments with Polysulfone (PSf) membranes coated with nanoFe304 were conducted to assess the removal of a model virus (bacteriophage MS2). The membranes were coated via a simple filtration protocol.Unmodified membranes were a poor adsorbent for MS2 bacteriophage with less than 0.5-log removal, whereas membranes coated with magnetite nanoparticles exhibited a removal efficiency exceeding 99.99% (4-log). Thus, a cartridge of PSf membranes coated with nano-Fe30 4 particles could be used to remove viruses from water. Such membranes showed negligible iron leaching into the filtrate, thus obviating concern about colored water. Further research is needed to reduce the loss of water flux caused by coating. ACKNOWLEDGEMENTS

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“…If the removal and inactivation of FRNAPH genotypes could be separately evaluated, the fate of the virus in the water environment or during the water treatment process would be better understood. Currently, the quantitative detection of Fϩ coliphages is done by plaque (culture) or reverse transcriptionquantitative PCR (RT-qPCR) assays (17)(18)(19)(20)(21)(22). The plaque assay can detect infectious phages but requires an immense effort for quantitative typing (23).…”

mentioning

confidence: 99%

Quantitative Distribution of Infectious F-Specific RNA Phage Genotypes in Surface Waters

Hata

Hanamoto

Shirasaka

et al. 2016

Appl Environ Microbiol

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F-specific RNA phages (FRNAPHs) are considered potential viral indicators of water pollution due to their occurrence and stability in water environments. However, their suitability as viral indicators is not fully elucidated because the characteristics of FRNAPHs are variable depending on the genotype. In this study, for the characterization of infectious FRNAPH genotypes, integrated culture reverse transcription-PCR coupled with the most probable number approach was applied to surface water samples. Further, to recover low concentrations of FRNAPH genotypes, an FRNAPH recovery method was developed. The novel FRNAPH recovery method using a noncharged microfiltration membrane could effectively recover FRNAPH strains without inactivation, while a method using an electronegative microfiltration membrane resulted in the inactivation of some strains. Infectious FRNAPH genotypes in surface water samples were successfully quantified with an efficiency comparable to that of the conventional plaque assay. Genotype I (GI) and GII FRNAPHs tended to be predominant at locations impacted by treated and untreated municipal wastewater, respectively. The numbers and proportions of infectious FRNAPHs tended to be higher during the winter season when water temperature decreased. IMPORTANCE Properties of FRNAPHs are highly variable depending on their genotypes. Previous typing methods for

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Efficiency of MS2 phage and Qβ phage removal by membrane filtration in water treatment: Applicability of real-time RT-PCR method

Cited by 85 publications

References 21 publications

Le réseau de distribution d’eau potable : un écosystème complexe lié à des enjeux de santé publique

Le réseau de distribution d’eau potable : un écosystème complexe lié à des enjeux de santé publique

Addition of a magnetite layer onto a polysulfone water treatment membrane to enhance virus removal

Quantitative Distribution of Infectious F-Specific RNA Phage Genotypes in Surface Waters

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