Efficiency of morphological trait descriptors in discrimination of<i>Ocimum basilicum</i>L. accessions

Carović‐Stanko, Klaudija; Šalinović, Ana; Grdiša, Martina; Liber, Zlatko; Kolak, Ivan; Šatović, Zlatko

doi:10.1080/11263504.2011.558677

Cited by 34 publications

(19 citation statements)

References 16 publications

(17 reference statements)

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“…The AFLP analysis generally supports the genetic background of the previously established green morphotypes (11). While the small-leaf (Samples 01 -04) and lettuce-leaf (Samples 05 -08) morphotypes of the green cultivars formed separate branches in the NJ tree, the true (Samples 09 -13) morphotype was found in two separate branches in the NJ tree.…”

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confidence: 78%

“…Here, we report the chemical characterization of 87 essential oil components by using gas chromatography/mass spectrometry (GC/MS) analysis [9], and the genetic characterization of these 27 cultivars using amplified fragment length polymorphism (AFLP) as a DNA fingerprinting technique [10]. Because the Ocimum basilicum accessions used in this study have been previously classified in six morphotypes [11], we could perform detailed comparisons of their chemistry, genetics, and morphology. Similar investigations of the genetic diversity, essential oil composition, and morphological variability of Ocimum species have been previously published [12 -22]; however, none of these studies involved the AFLP analysis of common basil cultivars, and in none of them so many chemical compounds with genetic relationships and morphology have been compared.…”

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confidence: 99%

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Chemical Characterization and Genetic Relationships among Ocimum basilicum L. Cultivars

Liber

Carović‐Stanko

Politeo

et al. 2011

Chemistry & Biodiversity

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Twenty-seven Ocimum basilicum cultivars were subjected to a chemical characterization of essential oil components by gas chromatography/mass spectrometry (GC/MS) and a genetic characterization using the amplified fragment-length polymorphism (AFLP) technique. Since the same 27 accessions had previously been classified into six morphotypes, these analyses allowed us to make detailed comparisons of chemistry, genetics, and morphology. The chemical composition and morphology of the studied cultivars appeared to have a strong genetic component. The AFLP analysis revealed a distinction between the green and purple morphotypes. The green morphotypes predominantly utilized the terpene biosynthetic pathway, while most purple morphotypes primarily utilized the phenylpropene biosynthetic pathway. The GC/MS analysis led to identification of 87 volatiles. Among the 27 cultivars, five chemotypes were identified. A detailed characterization of the essential oil constituents indicated the existence of both specific combinations of compounds and 'private' compounds with the potential to be used in many aspects of human life. The established relationship between a genetic profile, chemical composition, and morphology represents an important step in future breeding programs and in the cultivation of this species.

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confidence: 78%

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confidence: 99%

Chemical Characterization and Genetic Relationships among Ocimum basilicum L. Cultivars

Liber

Carović‐Stanko

Politeo

et al. 2011

Chemistry & Biodiversity

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“…Svecova and his groups (2010) studied 34 cultivars of basil (tulsi) using 13 morphological descriptors including plant-, leaf-and inflorescence characters; they observed that the studied basil cultivars displayed a wide diversity of morphological characteristics. In a similar study of identification of O. basilicum L. accessions, carried out by Carovic-Stanko et al (2011), it was concluded that morphological markers provide an inexpensive and reliable method for routine screening of a large number of accessions, as well as to map phylogeny among tusli germplasms. Erum et al (2011), comparing various quantitative plant and flower characters, also found that a high variation existed for several characters in tulsi.…”

Section: Variations Of the Morphological Charactersmentioning

confidence: 99%

“…Tewari et al (2012) reported an Ocimum species, O. kilimandscharicum from western Himalayan region and evaluated certain nutritional characters among its population of O. kilimandscharicum. Analyses of diversity among tulsi ecotypes have also been done using molecular markers by several groups (Omidbaigi et al 2010, Carovic-Stanko et al 2011. However, there is little information available regarding the plant's diversity in India and in Asia; there is confusion regarding even its genome structure.…”

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Population Polymorphism Study Among Tulsi (Ocimum sp.) Ecotypes In Assam, India Using Morphological And Biochemical Parameters

Borah

Chutia

Modi³

et al. 2015

International Journal of Crop Science and Technology

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The genus Ocimum, (family Lamiaceae), displays great variability. Prevalence of cross pollination, polyploidy and interspecific hybridization has complicated its taxonomy, making its systematic study difficult. This study investigated the morphological and biochemical variability among 18 ecotypes collected from different parts of the Assam state, India. Considerable diversity was found using all the approaches. The quantitative morphological parameters showed wide variability, not always co-relating with their geographical distances. The Euclidean distance ranged between 6.06 (between the closest accessions) and 59.00 (between the most distant accessions). Phylogenetic analysis divided the accessions into two major clusters sharing approx. 76% identity, and a minor cluster (with three accessions) sharing only approx. 65% identity with the other two. A great deal of diversity was found in the qualitative characters too; that divided the ecotypes into two to five groups. The biochemical characters also revealed wide polymorphism; the distance varied from 1.690 to 58.574. Similar to the morphological characters, the biochemical parameters too did not always correlate with geographical distances. The phylogenetic mapping divided the accessions into two major clusters, sharing approx. 80% similarity. Further work will be needed to correlate the distance based on the morphological and the biochemical characters. (Omidbaigi et al. 2010, Carovic-Stanko et al. 2011. However, there is little information available regarding the plant's diversity in India and in Asia; there is confusion regarding even its genome structure. The northeastern part of India, including the state of Assam, being a biodiversity hotspot, would be a good start point to study the diversity of the plant in the country as well as in the region. To our knowledge, this is the first report on the categorization of the morphological and biochemical parameters of the plant. MATERIALS AND METHODS Plant Materials and Growth ConditionsThe plant materials were collected from 18 different districts of Assam and were named as accession (Acc) #1 to accession #18 (Table 1; Fig 1). Seeds were also collected for all the ecotypes, and were germinated in laboratory on moist filter paper in Assam Agricultural University (AAU), Jorhat. The seedlings were grown in the potting mixture prepared by mixing soil, sand and dried manure in the ratio of 2:1:1 v/v, and the plants were maintained in typical greenhouse conditions (16/8 hours of light/dark cycle and 22-25°C temperature) in the AAU facility. The morphological data were recorded at the vegetative (150 day-old) stage of the plants for the leaf and the stem characters; the inflorescence characters were taken at the reproductive stage, at the 200 day-old plants. For collection of the biochemical data, leaf samples were collected at the same stage and were processed for isolation and/ or quantification of the parameters. Morphological ParametersDefined descriptors are not available in tulsi. Therefore, based on t...

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Effects of biochar and compost addition in potting substrates on growth and volatile compounds profile of basil (Ocimum basilicum L.)

Nocentini,

Mastrolonardo,

Michelozzi

et al. 2023

J Sci Food Agric

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BackgroundDespite the optimal characteristics of peat, more environmental‐friendly materials are needed in the nursery sector, which however must guarantee specific quantitative and qualitative commercial standards. In this work, we evaluated the influence of biochar and compost as peat surrogates on yield and essential oil profile of two different varieties of basil (Ocimum basilicum var. Italiano and O. basilicum var. minimum). In two 50‐days pot experiments, we checked the performances of biochar from pruning of urban trees and composted kitchen scraps, both mixed in different proportions with commercial peat (first experiment), and under different N fertilization regimes (second experiment), in terms of plant growth and volatile compounds profile of basil.ResultsTotal or high substitution of peat with biochar (100% and 50% v.v.) or compost (100%) resulted in seedlings death after few days from transplantation, probably because of the growing media's too high pH and electrical conductivity. Substrates with lower substitution rates (10‐20%) were underperforming in term of plant growth and color compared to pure commercial peat during the first experiment, while better performances were obtained by the nitrogen‐fertilized mixed substrates in the second experiment, at least for one variety. We identified a total of 12 and 16 aroma compounds of basil (mainly terpenes) in the two experiments. Partial replacement of peat did not affect basil volatile organic compounds content and composition, while N fertilization overall decreased the concentration of these compounds.ConclusionOur results support a moderate use of charred or composted materials as peat surrogates.This article is protected by copyright. All rights reserved.

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Efficiency of morphological trait descriptors in discrimination ofOcimum basilicumL. accessions

Cited by 34 publications

References 16 publications

Chemical Characterization and Genetic Relationships among Ocimum basilicum L. Cultivars

Chemical Characterization and Genetic Relationships among Ocimum basilicum L. Cultivars

Population Polymorphism Study Among Tulsi (Ocimum sp.) Ecotypes In Assam, India Using Morphological And Biochemical Parameters

Effects of biochar and compost addition in potting substrates on growth and volatile compounds profile of basil (Ocimum basilicum L.)

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