Efficiency of molecular markers in identifying fertility restoration trait of WA-CMS system in rice

Revathi, P.; Medoju, Pavani; Singh, Arun Kumar; Sundaram, R. M.; Raju, Sravan; Senguttuvel, P.; Kemparaju, K. B.; Hariprasad, A. S.; Ramesha, M. S.; Neeraja, C. N.; Rani, N. Shobha; Viraktamath, B. C.

doi:10.5958/j.0019-5200.73.1.012

Cited by 23 publications

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“…, Revathi et al. ). Categorization of varieties based on the allelic constitution at these two marker loci revealed that 13% contained dominant functional alleles of both Rf3 and Rf4 ( Rf3Rf4 ), 6% varieties carried only Rf3 dominant functional allele with recessive allele of rf4 ( Rf3rf4 ), 50% had Rf4 dominant functional allele with recessive allele of rf3 ( rf3Rf4 ), and 31% possessed recessive allele of both the genes in homozygous condition ( rf3rf4 ).…”

Section: Discussionmentioning

confidence: 99%

“…The Rf3 and Rf4 genes that were reported to restore male fertility of WA CMS system were mapped on chromosome 1 and 10, respectively , Jing et al 2001, Ahmadikhah and Karlov 2006, Alavi et al 2009). Two markers, RM6100 and DRRM Rf3-10, were already validated as tightly linked with fertility restoration of WA-based cytoplasm by Rf3 and Rf4 genes in rice (Singh et al 2005, Balaji Suresh et al 2012, Revathi et al 2013. Categorization of varieties based on the allelic constitution at these two marker loci revealed that 13% contained dominant functional alleles of both Rf3 and Rf4 (Rf3Rf4), 6% varieties carried only Rf3 dominant functional allele with recessive allele of rf4 (Rf3rf4), 50% had Rf4 dominant functional allele with recessive allele of rf3 (rf3Rf4), and 31% possessed recessive allele of both the genes in homozygous condition (rf3rf4).…”

Section: Discussionmentioning

confidence: 99%

“…Revathi et al. () have evaluated the efficiency of tightly linked markers of Rf3 and Rf4 genes for fertility restoration in which 85–92% efficiency was identified. However, they did not study the effect of individual genes or absence of both the genes through testcross with CMS lines.…”

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“…Additionally, partial restorer lines that have good agronomic background can be improved by transfer of fertility restorer genes without involving a sterile cytoplasm or extensive testcrossing with cytoplasmic male sterile (CMS) lines. Revathi et al (2013) have evaluated the efficiency of tightly linked markers of Rf3 and Rf4 genes for fertility restoration in which 85-92% efficiency was identified. However, they did not study the effect of individual genes or absence of both the genes through testcross with CMS lines.…”

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Frequency and fertility restoration efficiency of Rf3 and Rf4 genes in Indian rice

et al. 2017

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Identification of new parental lines is crucial for developing ecology-specific hybrids with ideal agronomic performance. We screened a total of 570 different ecology-specific Indian rice varieties for the presence of fertility restorer genes, Rf3 and Rf4 using tightly linked markers DRRM Rf3-10 and RM6100, respectively. Among these varieties, 13% carried Rf3Rf3/Rf4Rf4, 31% carried rf3rf3/rf4rf4, 6% carried Rf3Rf3/rf4rf4 and remaining 50% carried Rf4Rf4/rf3rf3 allelic combinations. A mini set of 40 varieties with variable allelic combinations of fertility restorer genes were testcrossed with WA and Kalinga-based CMS lines. All the 80 F 1 s were evaluated for spikelet fertility and fertility restoration ability. Rf3Rf3/rf4rf4 genotypes mostly behaved as partial maintainers or partial restorers. In contrast, rf3rf3/Rf4Rf4 genotypes were partial or effective restorers. However, double dominant genotypes showed better fertility restoration than the genotypes containing Rf3 or Rf4 individually. Some of the genotypes showed unexpected restoration pattern implying occurrence of other fertility restorer(s) apart from Rf3 and Rf4. The perfect restorers and maintainers identified in this study can be directly used in hybrid rice breeding.

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confidence: 99%

Section: Discussionmentioning

confidence: 99%

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confidence: 99%

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Frequency and fertility restoration efficiency of Rf3 and Rf4 genes in Indian rice

et al. 2017

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“…According to the male fertility of F 1 plants, the test lines can be classified as maintainers, restorers, partial restorers and partial maintainers 9 . Molecular markers linked to the fertility restoration trait are useful for evaluation of large number of germplasm/breeding lines to identify restorers in rice without progeny testing 18 . Based on molecular screening with markers linked to Rf4 and Rf3 genes 19 reports that lines possessing both the Rf genes showed higher fertility than the genotypes containing Rf3 or Rf4 individually.…”

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Conversion of partial restorer Swarna into restorer by transferring fertility restorer Rf gene(s) through marker assisted back cross breeding (MABB) in rice

Ponnuswamy

Singh

Raman

et al. 2020

Sci Rep

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the major constraints in hybrid rice breeding are availability of limited number of parental lines with specific desirable traits and lower frequency of restorers among elite breeding lines. The popular, high-yielding mega-rice variety Swarna, has been identified to be a partial restorer (as it has only one of major fertility restorer genes, Rf4) and hence cannot be utilized directly in the hybrid rice breeding. To convert the partial restorer to complete restorer, a cross was made between Swarna and a stable restorer KMR3R possessing Rf3 and Rf4 genes and developed BC 1 f 5 and Bc 2 f 4 populations by markerassisted back cross breeding (MABB). The SSR marker DRRM-RF3-10 linked to Rf3 gene located on chromosome 1, clearly distinguished restorers from partial restorers. All the improved lines of Swarna possessing Rf3 and Rf4 genes showed complete fertility restoration in test crosses with higher grain yield heterosis. few rice hybrids developed by using converted restorers were evaluated in multi location testing under the All india co-ordinated Rice improvement project (AicRip). the results indicated that new rice hybrids expressed higher heterosis with matching grain quality attributes like Swarna. This study provides significantly novel and relevant restorers to enhance and economize future hybrid rice breeding programs.Rice (O. sativa L.) is the most important staple food crop for more than half of the world's population and it is cultivated in an area of 44.5 million hectare in India with the production of 106.5 million tonnes during the year 2016 1 . After the advent of high yielding semi-dwarf rice varieties, hybrid rice technology has been touted as a major strategy for enhancing the genetic yield potential of rice. The success of hybrid rice technology has been very well demonstrated in China, which produces 146.5 million tonnes of rice from 30.32 million hectares 1 . This significant increase in production in China is mainly due to cultivation of hybrid rice (with >50% area and production under rice hybrids). Several technical challenges, market and policy constraints has limited the development and diffusion of hybrid rice outside China 2 . In India, hybrid rice is cultivated in an area of ~3 million hectares 3 , which is about 6.7% of total area of rice cultivation. Hybrid rice accounts for less than 10% of the area under rice cultivation in Bangladesh, Indonesia, and the Philippines and just 10% in Vietnam.Hybrid rice technology aims to increase the yield potential of rice by exploiting the phenomenon of hybrid vigour or heterosis. Cytoplasmic male sterility coupled with fertility restoration controlled by nuclear genes is a very useful tool in exploiting heterosis in self pollinated crops. In rice, three CMS systems viz. Wild Abortive (WA), Boro II (BT) and Honglian (HL) are deployed for commercial hybrid rice seed production 4,5 . The most widely used CMS system in rice is based on wild abortive (WA) cytoplasm derived from Oryza sativa f. Spontanea 6,7 . The WA-CMS system is highly stable with compl...

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Marker-assisted identification of restorer gene(s) in iso-cytoplasmic restorer lines of WA cytoplasm in rice and assessment of their fertility restoration potential across environments

Kumar

Bhowmick

Singh

et al. 2017

Physiol Mol Biol Plants

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Iso-cytoplasmic restorers possess the same male sterile cytoplasm as the cytoplasmic male sterile (CMS) lines, thereby minimizing the potential cyto-nuclear conflict in the hybrids. Restoration of fertility of the wild abortive CMS is governed by two major genes namely, and. Therefore, assessing the allelic status of these restorer genes in the iso-cytoplasmic restorers using molecular markers will not only help in estimating the efficiency of these genes either alone or in combination, in fertility restoration in the hybrids in different environments, but will also be useful in determining the efficacy of these markers. In the present study, the efficiency of molecular markers in identifying genotypes carrying restorer allele of the gene(s) and restoring male fertility of WA cytoplasm in rice was assessed in a set of 100 iso-cytoplasmic rice restorers using gene linked as well as candidate gene based markers. In order to validate the efficacy of markers in identifying the restorers, a sub-set of selected 25 iso-cytoplasmic rice restorers were crossed with four different cytoplasmic male sterile lines namely, IR 79156A, IR 58025A, Pusa 6A and RTN 12A, and the pollen and spikelet fertility of the Fs were evaluated at three different locations. Marker analysis showed that was the predominant fertility restorer gene in the iso-cytoplasmic restorers and had a synergistic effect on fertility restoration. The efficiency of gene based markers, DRCG-RF4-14 and DRRM-RF3-10 for (87%) and (84%) genes was higher than respective gene-linked SSR markers RM6100 (80%) and RM3873 (82%). It is concluded that the gene based markers can be effectively used in identifying fertility restorer lines obviating the need for making crosses and evaluating the Fs. Though gene based markers are more efficient, there is a need to identify functional polymorphisms which can provide 100% efficiency. Three iso-cytoplasmic restorers namely, PRR 300, PRR 363 and PRR 396 possessing both and genes and good fertility restoration have been identified which could be used further in hybrid rice breeding.

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Efficiency of molecular markers in identifying fertility restoration trait of WA-CMS system in rice

Cited by 23 publications

References 0 publications

Frequency and fertility restoration efficiency of Rf3 and Rf4 genes in Indian rice

Frequency and fertility restoration efficiency of Rf3 and Rf4 genes in Indian rice

Conversion of partial restorer Swarna into restorer by transferring fertility restorer Rf gene(s) through marker assisted back cross breeding (MABB) in rice

Marker-assisted identification of restorer gene(s) in iso-cytoplasmic restorer lines of WA cytoplasm in rice and assessment of their fertility restoration potential across environments

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