Efficiency of Kumquat Fruit (Fortunella margarita) Extract against Hepatotoxicity and Infertility Induced by Gamma Irradiation in Male Albino Rats

Abstract

I ONIZING radiation (IR) generate reactive oxygen species (ROS). Imbalance between ROS and antioxidants level leads to oxidative stress. Fortunella margarita (F. magarita) fruit contains a lot of bioactive compounds, such as polysaccharides, flavonoids, phenolic acids, ..etc. Therefore, it has high radical scavenging capacities and strong antioxidant activity. This study was designed to determine the probable efficiency of F. margarita extract against liver damage and sperm abnormalities induced by γ-radiation in male rats. Thirty six rats were divided into 6 groups (6 rats each): control group, treated groups: 14 days F. margarita group and 28 days F. margarita group, irradiated group (Irr): rats were exposed to 6Gy of whole body γ-radiation, pre-treated (14 days F. margarita extract + Irr) group and pre-and post-treated (28 days F. margarita extract + Irr) group. Biochemical investigations included lipid profile: total cholesterol (TC), triglycerides (TG), high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C), very low-density lipoprotein cholesterol (VLDL-C). Liver function enzymes involved analyzing serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), gamma-glutamyl transferase (GGT) level, hepatic tissue malondialdehyde (MDA), superoxide dismutase (SOD) and glutathione reduced (GSH) levels. Fertility competence (sperm head, tail and head and tail abnormalities) were recorded. Results obtained revealed the strong efficiency role of F. margarita fruit extract as an antioxidant against hepatotoxicity through enhancement of liver functions ( ALT, AST and GGt), reduction of oxidative stress (MDA, SOD and GSH), and powerful recovering of sperm abnormalities induced by γ-irradiation. Also, adjusting and normalization of TC, HDL-C, LDL-C, VLDL-C and TG have been shown.

التأثير الوقائي لمستخلص فاکهة الکمکوات الإيثانولي ضد تسمم الکبد الناتج عن الکاربيندازيم في الفئران

“…Administration rats with different concentrations of ethanol extract of kumquat caused a decrease in serum levels of ALT, AST, and TB an increase in TP. These results are in agreement with other studies (12,40) who illustrated that the levels of liver enzymes (ALT, AST, and GGT) in rats treated with ethanol extract of kumquat was significantly reduced. No significant differences were observed (p > 0.05) in AST between rats were given EEK (300 mg/kg of B.W) and the negative group.…”

“…Ethanol extract of kumquat had a capacity on DPPH radical by 14.51%. The high oxidation resistance of kumquat fruit extract may be attributed to the free radical scavenging activity (12). Kumquat could be considered as potential sources of flavonoids and phenolic compounds (38).…”

“…The antioxidant potential of kumquat fruit is due to its high phenolic and flavonoid contents with polyhydroxy groups (11). Kumquat fruit extract tends to prevent liver damage by maintaining the integrity of the plasma membrane, thereby suppressing the leakage of enzymes through membranes, exhibiting hepatoprotective activity (12). Therefore, this study aimed to evaluate the preventive effect of ethanol kumquat extract against carbendazim induced liver toxicity in rats.…”

Antioxidant and Anti Infertility Potency of Pomegranate (Punica granatum L.) in γ Irradiated Rats

“…The estimation of antioxidant levels and infertility factors provide a good guide for health hazards after irradiation. Accordingly, many researches and attempts have been executed to dispose these deleterious effects using natural sources of radiation protectors (Hassan et al, 2015;Abdallah et al, 2019).…”

Protective and Curative Effects of Fresh Orange Juice (Citrus Sinensis L.) Supplementation Against Liver Injuries, Hepatic Lipid, Protein, and Dnaoxidative Damage Induced Cyclooxygenase-2/Prostaglandin E2 Inflammatory Pathway in Female Irradiated Rats

“…IR exposure may lead to tissue damage, and in some cases, may cause death depending on different factors. These factors include absorbed dose, irradiation duration, interval after exposure, and its tendency to initiate a series of reactions that increase the levels of free radicals in cells and tissues [4].…”