Efficiency of Inter Simple Sequence Repeat (ISSR) markers for the assessment of genetic diversity of Moroccan pomegranate (Punica granatum L.) cultivars

Ajal, El Amine; Jbir, Rania; Melgarejo, Pablo; Hernández, Francisca; Haddioui, Abdelmajid; Salhi-Hannachi, Amel

doi:10.1016/j.bse.2014.04.003

Cited by 23 publications

(23 citation statements)

References 12 publications

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“…The outcomes of Jacard's dendrogram in both ISSR and SSR primers can indicate a problem of homonymy or synonymy in the cultivars appellation. Hence, despite the relative high degree of diversity, the Iranian pomegranate germplasm represents a quite homogenous population, similar results have been reported by Ajal et al, (2014); Nafees et al, (2015). In accordance with obtained results in this study there have been genetic differences within each cultivar, and these distinctions may be caused by different environmental conditions, wrong naming by pomegranate growers and mutation that cause the incorrect name.…”

Section: Discussionsupporting

confidence: 84%

“…The used ISSR primers showed a higher percentage of polymorphic bands of 83.23% compared to the finding in Iran by Noormohammadi et al (2012), (Narzary et al, 2010), Talebi Bedaf et al (2011), and Ajal et al, (2014 in Morocco. In addition, due to high PIC value obtained in the majority of ISSR and SSR markers, these markers could be used for identification and characterization of each pomegranate cultivar maybe, only due to the high number of SSR and ISSR loci studied, supporting the results obtained by Ebrahimi et al (2010); Hasnaoui et al (2010b); Pirseyedi et al (2010);Soriano et al (2011) that present low PIC values.…”

Section: Discussionmentioning

confidence: 53%

“…These results are in accordance to the findings of Narzary et al (2010) and may be due to the reason that these regions had a high genetic overlap as a result of relatively high gene flow. This reasoning is further strengthened by the clonal propagation of pomegranate accessions (Parvaresh et al 2012;Ajal et al 2014).…”

Section: Analysis Of Molecular Variance (Amova)mentioning

confidence: 94%

“…Inter-Simple Sequence Repeats (ISSR) analysis is considered as another efficient molecular marker, showing genetic variation in the wild pomegranate populations studied in Western Himalaya region (Narzary et al, 2010;and Ajal et al, 2014). Morphological, cytological and DNA markers (RAPD, AFLP, SSR and ISSR) have been used to evaluate the genetic variability of Iranian pomegranates.…”

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confidence: 99%

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Assessment of Genetic Variation within Commercial Iranian Pomegranate (Punica granatum L.) Cultivars, Using ISSR and SSR Markers

Madadi

Zamani

Fatahi

2017

Turkish JAF Sci.Tech.

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Pomegranate is one of the most important horticultural crops in Iran, and has been cultivated for thousands of years in this country. At this period due to selection of superior cultivars from nature or mutation emerged in these cultivars, and their vegetative propagation, substantial genetic variation has occurred within and among the cultivars. Thus, each cultivar may consist of different clones. According to this issue, diversity within four commercial cultivars of pomegranate was analyzed. Two molecular marker systems including ISSR and SSR were used to evaluate variability between 36 samples of four commercial cultivars. ISSR markers produced 114 amplification products, out of which 97 were polymorphic (83.23%). Mean resolving power was 2.96 for ISSR markers. 19 SSR molecular markers were used, 15 of which amplified polymorphic products, while the remaining ones monomorphic., The number of polymorphic alleles per locus ranged from two to four (average 3.6). The observed and expected heterozygosities ranged from 0.04 to 0.92 and 0.14 to 0.62, respectively. In addition, mean polymorphic information content was 0.45 for SSR loci. Our results showed that commercial Iranian pomegranate have different clones. Therefore, ISSR and SSR markers can be a useful tools for detecting clones of each cultivar.

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Section: Discussionsupporting

confidence: 84%

Section: Discussionmentioning

confidence: 53%

Section: Analysis Of Molecular Variance (Amova)mentioning

confidence: 94%

mentioning

confidence: 99%

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Assessment of Genetic Variation within Commercial Iranian Pomegranate (Punica granatum L.) Cultivars, Using ISSR and SSR Markers

Madadi

Zamani

Fatahi

2017

Turkish JAF Sci.Tech.

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“…Hence, our results are clearly distinguishing among the local and the international varieties of performance in Egypt along with three separate categories. Apparently, in view with the previous revisions, our results obtained are better than expected, along with the discrimination capacity and the level of polymorphism when compared to the previous studies in Iran, China, Morocco, Oman and Egypt (Moslemi et al, 2010;Alsadi et al, 2012;Zhao et al, 2013;Ajal et al, 2014;Sinjare, 2015;Al-Sadi et al, 2015;Ismail et al, 2014). In contrast with the previous study in Egyptian pomegranate cultivars created on ISSR and AFLP by Ismail et al, (2014).…”

Section: Discussioncontrasting

confidence: 50%

Utilization of ISTR, ISSR and SRAP molecular markers to reveal and classify Egyptian pomegranates (Punica granatum L.)

Amar¹,

El-Zayat²

2017

Plant Omics

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This study offers the comprehensive analysis of the genetic diversity among six local varieties (Manfalouti; Nab El-Gamal; Hegazy; Wardi; Assuity; Araby) and three international varieties (Wonderful; Marked Bani Rafie; Red Bani Rafie) of pomegranate in Egypt, based on the behavior of ISTR, ISSR and SRAP markers profiles. A set of 24 ISTR, 16 ISSR and 30 SRAP primers combination were compared, concerning exposed the degree of resolution and efficiency of the discriminating capacity technique alongside with the level of genetic polymorphism, to determine which varieties evolved from the others. Considering the results, SRAP assay had superior, more sensitive, higher discrimination capacity and gives much more evidence about the total number of effective alleles (1411), number of polymorphic amplicons (312), PIC (0.94), assay efficiency index (47.04), effective multiples ratio (10.04) and marker index (9.74). Unexpectedly, the ISTR profile demonstrated a significant moderate level of polymorphism among tested genotypes. To further determine the genetic relationships and the distance among varieties, a graphic demonstration of combined UPGMA tree and the PCA analysis did assemble with clear resolution and accurate along with three categories.Our findings confirm that combining different marker system were greatly better and more effective considered an important priority step toward diversity study and characterization. We can point out that, the two-widespread varieties in Egypt Manfalouti, and Nab El-Gamal, formed a high homogeneity in genetic similarity with the Wonderful, as an essential global species for breeding programs, efforts widening the genetic base of pomegranates and the introduction of new genotypes in Egypt.

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Current Fungal Taxonomy and Developments in the Identification System

Lin,

Kook,

et al. 2023

Curr Microbiol

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Efficiency of Inter Simple Sequence Repeat (ISSR) markers for the assessment of genetic diversity of Moroccan pomegranate (Punica granatum L.) cultivars

Cited by 23 publications

References 12 publications

Assessment of Genetic Variation within Commercial Iranian Pomegranate (Punica granatum L.) Cultivars, Using ISSR and SSR Markers

Assessment of Genetic Variation within Commercial Iranian Pomegranate (Punica granatum L.) Cultivars, Using ISSR and SSR Markers

Utilization of ISTR, ISSR and SRAP molecular markers to reveal and classify Egyptian pomegranates (Punica granatum L.)

Current Fungal Taxonomy and Developments in the Identification System

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