Efficiency of direct and indirect shoot organogenesis, molecular profiling, secondary metabolite production and antioxidant activity of micropropagated Ceropegia santapaui

Chavan, Jaykumar J.; Gaikwad, Nikhil B.; Umdale, Suraj D.; Kshirsagar, Parthraj R.; Bhat, K. V.; Yadav, S. R.

doi:10.1007/s10725-013-9830-7

Cited by 55 publications

(24 citation statements)

References 44 publications

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“…5d), while primer OPV-18 showed 4 additional bands in clone-6 and clone-7. A variation during micropropagation depends upon the source of explants and the mode of in vitro regeneration [30], [31]. In the present study, high levels of uniformity among donor and in vitro raised clones was observed.…”

Section: Resultssupporting

confidence: 54%

Highly efficient in vitro regeneration, establishment of callus and cell suspension cultures and RAPD analysis of regenerants of Swertia lawii Burkill

Kshirsagar

Chavan

Umdale

et al. 2015

Biotechnology Reports

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Section: Resultssupporting

confidence: 54%

Highly efficient in vitro regeneration, establishment of callus and cell suspension cultures and RAPD analysis of regenerants of Swertia lawii Burkill

Kshirsagar

Chavan

Umdale

et al. 2015

Biotechnology Reports

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“…The results presented in Table 7 show that the average content of 20-HE in leaves and roots of IDSO-plants were found to be 3-and 1.5-times higher than those in leaves and roots of DSO-plants (7.4 mg g -1 DW and 3.04 mg g -1 DW vs. 2.5 and 2.1 mg g -1 DW, respectively). Chavan et al (2014) also note the presence of quantitative differences between Ceropegia santapaui plants regenerated through indirect and direct organogenesis, with the former exhibiting increased levels of total phenolics and flavonoids. So far, the biosynthetic differences have not been well clarified.…”

Section: Quantification Of Chlorogenic Acid and 20-hydroxyecdysonementioning

confidence: 77%

“…So far, the biosynthetic differences have not been well clarified. Chavan et al (2014) suggest that these might be due to the exogenous supply of the plant growth regulators during in vitro multiplication.…”

Section: Quantification Of Chlorogenic Acid and 20-hydroxyecdysonementioning

confidence: 99%

Rhaponticum carthamoides regeneration through direct and indirect organogenesis, molecular profiles and secondary metabolite production

Skała

Grąbkowska

Sitarek

et al. 2015

Plant Cell Tiss Organ Cult

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The organogenic competence of different explants of Rhaponticum carthamoides was investigated on MS agar medium supplemented with BA, IBA or NAA at concentrations of 0.2 and 0.5 mg L -1 . Adventitious shoot formation was obtained through direct organogenesis using leaves of in vitro cultures as explants and through indirect organogenesis when seedling explants (hypocotyl, cotyledon and root) were used for regenerative callus initiation. The shoots were rooted on half-strength MS medium ( MS) without auxin or containing IBA (0.2-2.0 mg L -1 ). The plantlets regenerated through direct and indirect organogenesis were transferred into pots and grown in the greenhouse for 3 months. Significant differences in morphology, accumulation of chlorogenic acid and 20-hydroxyecdysone (20-HE) as well as in genetic profile were observed between these plants. UHPLC analysis showed that the highest level of chlorogenic acid (12 mg g -1 DW) was found in leaves of plants developed directly from explants, whereas leaves of plants developed via callus tissue accumulated the highest amount of 20-HE (7.4 mg g -1 DW). Its level exceeded that detected in leaves of 3-month-old plants obtained from seeds (2.4 mg g -1 DW). Genetic variations of R. carthamoides regenerated plants were evaluated by flow cytometry and RAPD and ISSR methods. Flow cytometry confirmed similar ploidy level in the mother plant and plants regenerated through direct and indirect organogenesis. Genetic similarity values calculated on the basis of RAPD and ISSR data among regenerated in vitro plants to the mother plant were ranged from 0.765 to 0.941 and 0.647 to 0.947, respectively.

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“…The possible reasons for polymorphism observed during analysis that occurred in in-vitro raised plantlets regenerated from mother plants using indirect organogenesis might be because of source of application of growth regulators during callus induction, shoot regeneration, duration of cell, and tissue multiplication, accumulating mutations during the process of indirect organogenesis, etc. (Goel et al 2009;Rizvi et al 2012;Chavan et al 2014;Kshirsagar et al 2015;Thorat et al 2017). Similarly, ISSR primers were competent to detect polymorphism in Camellia chinensis (Devarumath et al 2002), Stevia (Martin et al 2004), Gerbera jamesonii (Bhatia et al 2011), Tylophora indica (Sharma et al 2014), Dendrocalamus strictus (Goyal et al 2016), Morus alba (Saha et al 2016) and Saccharum offi cinarum (Thorat et al 2017).…”

Section: Resultsmentioning

confidence: 99%

Plant regeneration from direct and indirect organogenesis and assessment of genetic fidelity in Saccharum officinarum using DNA-based markers

Thorat¹,

Sonone²,

Choudhari³

et al. 2018

Biosci. Biotech. Res. Comm

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Sugarcane has acquired signifi cant importance in the world economy because of the sugar and ethanol production. Therefore, rapid multiplication of outstanding sugarcane variety is necessary in developing countries. The aim of this investigation was to compare regeneration effi ciency of various explants and genetic fi delity of regenerated plantlets certifi ed by using DNA-based molecular markers, that is, random amplifi ed length polymorphism (RAPD) and inter simple sequence repeats (ISSR). The sugarcane plantlets were obtained through direct (axillary buds, apical meristem, and leaf whorl disk) and indirect (callus culture) shoot organogenesis from the variety Co 86032. Among all the explants, highest shoot forming ability was observed in axillary buds showed 97.66±0.66% shoot formation and the highest number of shoots per explants (4.33±0.24) and a total number of regenerated shoots (173.00±8.11) were observed in the leaf whorl disk. Morphological variation was not observed among the regenerated plants from various explants and therefore, genomic DNA was isolated from fresh leaves and genetic fi delity assessment was carried out using RAPD and ISSR. Both the markers produced 1368 and 2271 bands, respectively, including all the tested plants, indicates that plants derived from direct organogenesis did not show any polymorphism. However, a genetic variation has been observed in the plants derived from callus and showed 4.54% polymorphism during analysis. The results suggested that plants regenerated from direct organogenesis are of more true-to-type, whereas genetic variation occurs during indirect organogenesis. Combination of RAPD along with ISSR can be used for detection of genetic variation in the early stage in sugarcane micropropagation. High performance of regeneration and low risk of genetic variation ascertains the effi ciency of this operation.

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Efficiency of direct and indirect shoot organogenesis, molecular profiling, secondary metabolite production and antioxidant activity of micropropagated Ceropegia santapaui

Cited by 55 publications

References 44 publications

Highly efficient in vitro regeneration, establishment of callus and cell suspension cultures and RAPD analysis of regenerants of Swertia lawii Burkill

Highly efficient in vitro regeneration, establishment of callus and cell suspension cultures and RAPD analysis of regenerants of Swertia lawii Burkill

Rhaponticum carthamoides regeneration through direct and indirect organogenesis, molecular profiles and secondary metabolite production

Plant regeneration from direct and indirect organogenesis and assessment of genetic fidelity in Saccharum officinarum using DNA-based markers

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