Efficiency of different marker systems for genotype fingerprinting and for genetic diversity studies in barley (Hordeum vulgare L.)

Mészáros, Katalin; Karsaï, I.; Kuti, C.; Bányai, Judit; Láng, László; Bedö, Z.

doi:10.1016/j.sajb.2006.06.006

Cited by 21 publications

(16 citation statements)

References 25 publications

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“…Molecular markers such as RAPD (Fernández et al, 2002;Meszaros et al, 2007), AFLP (Zhang and Ding, 2007), ISSR (Fernández et al, 2002), STS (Meszaros et al, 2007), and SSR (Turuspekov et al, 2001;Matus and Hayes, 2002;Feng et al, 2006;Meszaros et al, 2007; can be used to estimate genetic diversity.…”

Section: Introductionmentioning

confidence: 99%

“…There have been many studies on genetic diversity in barley (Graner et al, 1994;Turuspekov et al, 2001;Matus and Hayes, 2002;Fernández et al, 2002;Feng et al, 2006;Meszaros et al, 2007;Zhang and Ding, 2007;Mikel and Kolb, 2008); however, little is known about the genetic diversity in barley landraces, especially for barley landraces from the Shanghai area. SSRs are codominant, abundant, and informative and their detection is very simple (Matus and Hayes, 2002).…”

Section: Introductionmentioning

confidence: 99%

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Genetic diversity analysis of barley landraces and cultivars in the Shanghai region of China

Chen

Lu²,

Zou³

et al. 2012

Genet. Mol. Res.

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ABSTRACT. We analyzed the genetic diversity of 115 barley germplasms, including 112 landraces and three new barley cultivars grown in the Shanghai region, using a set of 11 SSR markers. Sixtysix alleles were observed at the 11 SSR loci, ranged from three to ten, with a mean of six alleles per locus. The polymorphism information content ranged from 0.568 to 0.853, with a mean of 0.732, indicating considerable genetic variation in barley in the Shanghai area. Clustering analysis indicated that these barley accessions could be divided into two categories (A and B). Ninety-seven six-rowed barley cultivars were classified in the A category; sixteen two-rowed and two six-rowed barley cultivars were classified in the B category. This demonstrated genetic differences between two-rowed and six-rowed barley varieties. In addition, we found that the three new barley cultivars are closely related.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Genetic diversity analysis of barley landraces and cultivars in the Shanghai region of China

Chen

Lu²,

Zou³

et al. 2012

Genet. Mol. Res.

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“…Polymorphic bands of a given RAPD primer may bind to many parts of the genome, so each primer may give information on the polymorphism of several chromosome regions. ISSR prim-ers also provide a large quantity of polymorphic information, but this very detailed information only originates from a single hypervariable section of the genome (Meszaros et al, 2007). Also, higher mutation rate in the ISSR loci may be the reason for observing larger number of alleles in this marker (Moghaddam et al, 2009).…”

Section: Comparison Of Rapd and Issr Markersmentioning

confidence: 99%

“…In addition to the estimation of degrees of relationship between different varieties, a further important use of these markers is to distinguish between genotypes. Numerous molecular markers have been used for variety identification in various plant species, which A allow cultivar identification in early stages of plant development, being neutral to environmental effects (Mohammadi, 2002;Meszaros et al, 2007;Moghaddam et al, 2009). A variety of molecular markers including Restriction Fragment Length Polymorphism (RFLP) (Thormann et al, 1994), Inter-Simple Sequence Repeats (ISSR) (Carolyn et al, 2000;Rudolph et al, 2002), amplified fragment length polymorphism (AFLP) (Sandip et al, 1999;Seyis et al, 2003;Jiang et al, 2007) and random amplified polymorphic DNA (RAPD) (Ashik Rabbani et al, 1998;Lazaro and Aguinagalde, 1998;Divaret et al, 1999), have been used to study the extent of genetic variation among the diverse group of important crop species in the genus Brassica (Afiah et al, 2007;Marjanovic-jeromela et al, 2009).…”

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confidence: 99%

EFFICIENCY OF RAPD AND ISSR MARKERS FOR GENOTYPE FINGERPRINTING AND GENETIC DIVERSITY STUDIES IN CANOLA (Brassica napus)

Migid¹

2011

Egyptian Journal of Genetics and Cytology

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“…The development of molecular markers makes it easy to assess genetic diversity in crops at DNA level (Reif et al, 2003). Molecular markers such as RAPD (Fernández et al, 2002;Meszaros et al, 2007), AFLP (Zhang and Ding, 2007a), ISSR (Fernández et al, 2002), STS (Meszaros et al, 2007) and SSR (Turuspekov et al, 2001;Matus and Hayes, 2002;Feng et al, 2006;Meszaros et al, 2007;Zhang et al, 2007b) can be used to estimate genetic diversity. Ramsay et al (2000) developed SSR markers for molecular characterization and linkage mapping of barley.…”

Section: Introductionmentioning

confidence: 99%

Assessment of genetic diversity among released and elite Ethiopian barley genotypes using simple sequence repeat (SSR) markers

Misganaw¹,

Kidane²,

Tesfu³

2017

Afr. J. Plant Sci.

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Barley is a major cereal grown widely and used in several food products, beverage production and animal feed. Being the fourth most important cereal crop in the world and the fifth rank in Ethiopia, it is a cash crop and used as a source of malt by the brewery industries, as food for human and feed for animals. Genetic diversity assessment is a key component in breeding programs. High level of polymorphism, codominant and multi allelic nature of simple sequence repeats (SSRs) markers make them preferable for diversity analysis in plant species. In this study, 22 SSRs markers were used to characterize the genetic diversity of 39 released and elite barley varieties collected from barley breeding program in Ethiopia. The amplification of SSRs loci were obtained for 35 primer pairs and only 22 of them showed clear polymorphic patterns which produced a total of 73 alleles with an average of 5 alleles per locus. The data generated by these informative primers were sufficient to discriminate the analysed barley genotypes. Based on the dissimilarity matrices ranging from 0.11 to 0.58, the genotypes were grouped into three major groups. The calculated polymorphism information content (PIC) values ranges from 0.17 to 0.60 with an average of 0.47 which shows the importance of the markers for future diversity analysis of barley. Locus HVACL1 and HVM36 shows higher PIC and locus HVBDHN7 shows lower PIC in this characterized barley genotype. This result will be useful for barley germplasm management and improvement in terms of biodiversity protection and design of new crosses for future breeding purpose.

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Efficiency of different marker systems for genotype fingerprinting and for genetic diversity studies in barley (Hordeum vulgare L.)

Cited by 21 publications

References 25 publications

Genetic diversity analysis of barley landraces and cultivars in the Shanghai region of China

Genetic diversity analysis of barley landraces and cultivars in the Shanghai region of China

EFFICIENCY OF RAPD AND ISSR MARKERS FOR GENOTYPE FINGERPRINTING AND GENETIC DIVERSITY STUDIES IN CANOLA (Brassica napus)

Assessment of genetic diversity among released and elite Ethiopian barley genotypes using simple sequence repeat (SSR) markers

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