Efficiency of calcium phosphate composite nanoparticles in targeting Ehrlich carcinoma cells transplanted in mice

Abstract

The present study aimed to investigate the mode of action of nano-CaPs in vivo as a therapy for solid tumor in mice. To achieve this goal, Ehrlich Ascites Carcinoma (EAC) was transplanted into 85 Swiss male albino mice. After nine days, the mice were divided into 9 groups. Groups 1 and 2 were allocated as the EAC control. Groups 3 and 4 were injected once intratumorally (IT) by nano-calcium phosphate (nano-CaP). Groups 5 and 6 received once intraperitoneal injection (IP) of nano-CaP. Groups 7, 8, and 9 received nano-CaP (IP) weekly. Blood samples and thigh skeletal muscle were collected after three weeks from groups 1, 3, 5, and 7 and after four weeks from groups 2, 4, 6, and 8. On the other hand, group 9 received nano-CaP (IP) for four weeks and lasted for three months to follow up the recurrence of tumor and to ensure the safety of muscle by histopathological analysis. Tumor growth was monitored twice a week throughout the experiment. DNA fragmentation of tumor cells was evaluated. In thigh tissue, noradrenaline, dopamine, serotonin (5HT), and gamma-aminobutyric acid (GABA) were measured. In serum, 8-Hydroxy-deoxyguanosine (8-OHDG), adenosine triphosphate (ATP), and vascular endothelial growth factor (VEGF) were analyzed. Histopathological and biochemical results showed a significant therapeutic effect of nano-CaP on implanted solid tumor and this effect was more pronounced in the animals treated IP for four weeks. This improvement was evident from the repair of fragmented DNA, the significant decrease of caspase-3, 8-OHDG, myosin, and VEGF, and the significant increase of neurotransmitters (NA, DA, 5HT, and GABA). Additionally, histopathological examination showed complete recovery of cancer cells in the thigh muscle after three months.

Calcium phosphate adjuvanted nanoparticles of outer membrane proteins of Salmonella Typhi as a candidate for vaccine development against Typhoid fever

“…The calcium phosphate adjuvanted Omps nanoparticles formulated in this study had a size of 20–50 nm and were spherical in morphology. Similar nanoparticles were used earlier for drugs and antigen delivery [25, 38, 39]. Though in few reports, variation in size of nanoparticles was reported due to differences in frequency of stirring and pH of the suspension [24].…”

Apoptotic Responses Mediated by Nostoc-Synthesized Silver Nanoparticles against Ehrlich Ascites Carcinoma Tumor-Bearing Mice

“…Oxidative stress markers (GSH; (OD 412 ), GPx (OD 340 ); CAT (OD 520 )) and a lipid peroxidation marker (MDA; OD 532 ) were analyzed in vehicle control and N-SNPs-treated group tissues as previously mentioned [6,33,40,41]. In brief, the extracted EAC tumors were washed several times with ice-cold phosphate buffer saline and tissue lysates were prepared by homogenizing in lysis buffer (50 mM Tris, pH 7.5, 150 mM NaCl, 1% Triton X-100, 0.1% sodium dodecyl sulfate (SDS)).…”

Lichens—A Potential Source for Nanoparticles Fabrication: A Review on Nanoparticles Biosynthesis and Their Prospective Applications

“…Keskin et al demonstrated that cell-free culture media of Synechococcus sp. had a reducible activity that resulted, under light conditions, in the formation of Ag-NPs with an average nanosize of 140 nm [ 64 ]. However, this process is sometimes unsuitable for NP synthesis because many types of media used for the culture of microorganisms contain components that act as reductants and stabilizing agents.…”