Efficiency of a recombinant MSA-2c-based ELISA to establish the persistence of antibodies in cattle vaccinated with Babesia bovis

Bono, M.; Mangold, Atilio J.; Baravalle, María Eugenia; Valentini, Beatriz; Thompson, Carolina S.; Wilkowsky, Silvina Elizabeth; Echaide, Ignacio; Farber, Marisa Diana; Echaide, Susana Marta Torioni de

doi:10.1016/j.vetpar.2008.07.025

Cited by 17 publications

(14 citation statements)

References 20 publications

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“…This result corresponds well to the detection of anti-MSA-2c antibodies up to at least month 4 postinfection (p.i. ), as has been reported for an indirect-ELISA format based on the antigen (6).…”

Section: Resultssupporting

confidence: 70%

“…Serological assays are currently most likely to meet these requirements, as B. bovis infection finally leads to an asymptomatic carrier animal state in which the parasite commonly escapes direct detection by PCR, reverse line blot hybridization (RLB), or Giemsa-stained blood smears (10,23). A number of serological methods have been established for the detection of B. bovis-specific antibodies, of which ELISA is considered the most advantageous for epidemiological investigations, as it offers greater sensitivity and objectivity and can be easily adapted to test large numbers of serum samples (3,4,6,7,8,11,20,21,27,31,35,37). Furthermore, this format is amenable to the use of recombinant antigens, which makes it independent of the necessity to produce large amounts of parasites in vivo or in vitro, facilitates standardization, and has the potential to overcome limitations caused by cross-reactivity (22,32).…”

Section: Resultsmentioning

confidence: 99%

“…Furthermore, this format is amenable to the use of recombinant antigens, which makes it independent of the necessity to produce large amounts of parasites in vivo or in vitro, facilitates standardization, and has the potential to overcome limitations caused by cross-reactivity (22,32). The use of rMSA-2c as a diagnostic antigen in an indirect-ELISA format has been reported, and it has proved useful in the study of the development of antibody titers after experimental infection of bovines (6). However, the indirect-ELISA formats based on rMSA-2c suffer from low specificity and sensitivity and have not been recommended for field studies (6,35).…”

Section: Resultsmentioning

confidence: 99%

“…The use of rMSA-2c as a diagnostic antigen in an indirect-ELISA format has been reported, and it has proved useful in the study of the development of antibody titers after experimental infection of bovines (6). However, the indirect-ELISA formats based on rMSA-2c suffer from low specificity and sensitivity and have not been recommended for field studies (6,35).…”

Section: Resultsmentioning

confidence: 99%

“…MSA-2c is a species-specific, immunodominant antigen and the most conserved member of this family, showing very high amino acid sequence identity among B. bovis strains from Argentina, the United States, Mexico, and Australia (12,19,38). These features encouraged the use of MSA-2c for the development of serological tests, like an indirect enzyme-linked immunosorbent assay (ELISA) and a rapid immunochromatographic diagnostic test (6,26). A competitive ELISA (cELISA) is an adequate serological tool for the epidemiological surveillance of the spread of bovine babesiosis, as it can be easily standardized, is less laborious and less time-consuming than the traditionally used indirect immunofluorescence assay (IFAT) (immunofluorescence antibody test), and, in addition, has the potential to display higher specificity than an indirect ELISA.…”

mentioning

confidence: 99%

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Validation and Field Evaluation of a Competitive Enzyme-Linked Immunosorbent Assay for Diagnosis of Babesia bovis Infections in Argentina

Dominguez

Echaide²,

Echaide³

et al. 2012

Clin Vaccine Immunol

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ABSTRACTInfections byBabesia bovislimit cattle production and cause important economic losses in tropical and subtropical areas around the world. Monitoring of calf sera can be used to detect unprotected cattle herds and to decide on strategic control measures, as well as for epidemiological studies. Merozoite surface antigen 2c (MSA-2c) is an immunodominant surface protein expressed inB. bovismerozoites and sporozoites and contains B-cell epitopes that are conserved among geographic isolates. A monoclonal antibody against recombinant MSA-2c (rMSA-2c) was previously shown to inhibit the binding of anti-B. bovisantibodies to a parasite B-cell epitope in a competitive enzyme-linked immunosorbent assay (cELISA) format. In the work at hand, the parameters of this cELISA were reevaluated and adjusted when necessary, and a cutoff value was determined by receiver operator characteristic (ROC) curve analysis of a total of 357 bovine sera of known reactivity, as assessed by indirect immunofluorescence assay (IFAT). The established rMSA-2c cELISA demonstrated a specificity of 98% and a sensitivity of 96.2%. An additional set of 303 field bovine sera from regions where ticks are endemic and tick-free regions of Argentina was tested by both rMSA-2c cELISA and IFAT, and the results were shown to be in very good agreement (kappa index, 0.8325). The performance shown by rMSA-2c cELISA in the detection ofB. bovis-specific antibodies and its suitability for standardization and large-scale production, as well as the possibility of its application in most veterinary diagnostic laboratories, make the assay a powerful tool for the surveillance of herd immunity as a strategic measure for the control of bovine babesiosis.

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Section: Resultssupporting

confidence: 70%