Efficiency of a Rapid Test for Detection of Tetrodotoxin in Puffer Fish

Thattiyaphong, Aree; Unahalekhaka, Jirapa; Mekha, Nanthawan; Nispa, Wansatip; Kluengklangdon, Panawan; Rojanapantip, Laddawan

doi:10.1080/15321819.2013.802698

Cited by 8 publications

(12 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A method LOD of 0.1 µg/g tissue and toxin recoveries of 77%–81% were also reported. This method was later extended with TTX quantitation using MS/MS [ 80 ], with others using TTX as an internal standard [ 64 ]. MS/MS was used to confirm fragment ion spectra for each of the TTXs, with each showing characteristic fragmentation patterns, enabling the generation of a quantitative LC-MS/MS method for TTXs.…”

Section: Toxin Testing Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Potential Threats Posed by Tetrodotoxins in UK Waters: Examination of Detection Methodology Used in Their Control

et al. 2015

View full text Add to dashboard Cite

Tetrodotoxin is a neurotoxin responsible for many human fatalities, most commonly following the consumption of pufferfish. Whilst the source of the toxin has not been conclusively proven, it is thought to be associated with various species of marine bacteria. Whilst the toxins are well studied in fish and gastropods, in recent years, there have been a number of reports of tetrodotoxin occurring in bivalve shellfish, including those harvested from the UK and other parts of Europe. This paper reviews evidence concerning the prevalence of tetrodotoxins in the UK together with methodologies currently available for testing. Biological, biomolecular and chemical methods are reviewed, including recommendations for further work. With the recent development of quantitative chromatographic methods for these and other hydrophilic toxins, as well as the commercial availability of rapid testing kits, there are a number of options available to ensure consumers are protected against this threat.

show abstract

Section: Toxin Testing Methodsmentioning

confidence: 99%

“…A rapid TTX-detection test (TTX-IC) incorporating lateral-flow immunochromatography provides another approach for fast analysis of large numbers of potentially contaminated fish samples [ 80 ]. This, the first TTX lateral-flow assay, was developed in Thailand for rapid screening (5 min) of tissue samples.…”

Section: Toxin Testing Methodsmentioning

confidence: 99%

Potential Threats Posed by Tetrodotoxins in UK Waters: Examination of Detection Methodology Used in Their Control

et al. 2015

View full text Add to dashboard Cite

show abstract

“…Immunochromatographic strips have also been developed with the aim of providing a rapid visual test for the screening of TTX [ 229 , 230 , 231 ]. These assays, prepared on an absorbent pad to favor lateral flow, are based on the competition between the TTX from the sample and the TTX-BSA immobilized on the test line for a colloidal gold-labelled anti-TTX Ab.…”

Section: Biosensors Biochemical Assays and Cell-based Assaysmentioning

confidence: 99%

“…Although this method is not quantitative, it has the advantages of simplicity, ease of use, no sophisticated equipment requirement and short analysis times (10 min). This test has also been used in the screening of fish, establishing an action limit of 2 mg/kg [ 231 ]. The comparison with LC-MS/MS analysis has demonstrated the applicability of strip tests to the analysis of naturally-contaminated fish samples.…”

Section: Biosensors Biochemical Assays and Cell-based Assaysmentioning

confidence: 99%

Alternative Methods for the Detection of Emerging Marine Toxins: Biosensors, Biochemical Assays and Cell-Based Assays

et al. 2014

View full text Add to dashboard Cite

The emergence of marine toxins in water and seafood may have a considerable impact on public health. Although the tendency in Europe is to consolidate, when possible, official reference methods based on instrumental analysis, the development of alternative or complementary methods providing functional or toxicological information may provide advantages in terms of risk identification, but also low cost, simplicity, ease of use and high-throughput analysis. This article gives an overview of the immunoassays, cell-based assays, receptor-binding assays and biosensors that have been developed for the screening and quantification of emerging marine toxins: palytoxins, ciguatoxins, cyclic imines and tetrodotoxins. Their advantages and limitations are discussed, as well as their possible integration in research and monitoring programs.

show abstract

“…Thattiyaphong et al, (2014), performed a comprehensive study on pufferfish TTX extraction. They found out that the highest concentrations of TTX accumulated in the gonads followed by the liver of the pufferfish.…”

Section: Introductionmentioning

confidence: 99%

Maturation and Gonad Development of Yellowspotted Puffer Torquigener flavimaculosus (Osteichthyes: Tetraodontidae) from Iskenderun Bay, North-eastern Mediterranean

Yalnız¹,

Turan²,

Doğdu³

2017

Natural and Engineering Sciences

View full text Add to dashboard Cite

Marine pufferfish of the family Tetraodontidae accumulate the highest levels of tetrodotoxin (TTX) in the ovary. The level of TTX accumulation is reported to differ between males and females and fluctuate through gonadal development and maturation stages. Therefore, in the present work, maturation and gonad development of Yellowspotted Puffer (Torquigener flavimaculosus) from Iskenderun Bay, North-eastern Mediterranean were investigated histologically and morphologically. Mean length and weight of specimens were 12.1±0.58 cm and 20.25±0.17 g respectively. Histological examination of the gonads showed that maturation occurs in every single male T. flavimaculosus collected in summer 2017. Moreover, vitellogenic and matured oocytes were also consistently found in every female collected during the summer months. These data suggest that both sexes are reproductively active at the same time of the year. Where the spawning season for both males and females was detected in summer. T. flavimaculosus was found to be dioecious. Five and six developmental stages were indicated for testis and ovaries, respectively. The developmental pattern of ovaries was categorized as the asynchronous or group synchronous type.

show abstract

Efficiency of a Rapid Test for Detection of Tetrodotoxin in Puffer Fish

Cited by 8 publications

References 14 publications

Potential Threats Posed by Tetrodotoxins in UK Waters: Examination of Detection Methodology Used in Their Control

Potential Threats Posed by Tetrodotoxins in UK Waters: Examination of Detection Methodology Used in Their Control

Alternative Methods for the Detection of Emerging Marine Toxins: Biosensors, Biochemical Assays and Cell-Based Assays

Maturation and Gonad Development of Yellowspotted Puffer Torquigener flavimaculosus (Osteichthyes: Tetraodontidae) from Iskenderun Bay, North-eastern Mediterranean

Contact Info

Product

Resources

About