Efficiency enhancement of a new cellulase cocktail at low enzyme loading for high solid digestion of alkali catalyzed atmospheric glycerol organosolvent pre-treated sugarcane bagasse

Nwamba, Marknoah Chinenye; Song, Guojie; Sun, Fubao; Mukasekuru, Marie Rose; Ren, Hongyan; Zhang, Qing; Cao, Tishuang; Wang, Huaming; Sun, Haiyan; Hong, Jiong

doi:10.1016/j.biortech.2021.125505

Cited by 25 publications

(19 citation statements)

References 43 publications

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“…Add 10 mL 50 mmol/L pH 4.8 citric acid buffer (solid-liquid ratio: 10%) with 1 g straw, and make cellulase loads of 2 mg/g, 5 mg/g, 10 mg/g, 15 mg/g and 20 mg/g (enzyme protein/corn straw), respectively. Put 50 mL centrifugal tube into a constant temperature shaking table at 50°C and 180 rpm for 72 h (Nwamba et al, 2021). Complete cellulase hydrolysis adjusted the substrate solid liquid ratio to 1% with excess enzyme, and the reaction time was 24 h. Other conditions remained unchanged.…”

Section: Cellulase Hydrolysis After Pretreatmentmentioning

confidence: 99%

A newly isolated lignin degradation bacterium Acinetobacter sp. B213 pretreatment effectively improved hydrolysis of corn straw by enhance cellulose retention and reduce lignin degradation inhibitor

Zhang

Liu

et al. 2022

Preprint

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An improvement of lignin depolymerization while reducing cellulose losses during the pretreatment by microorganisms gains an interest in the highly efficient utilization of lignocellulosic biomass. Here, a strain isolated from activated sludge could utilize lignin as the sole carbon source and identify as Acinetobacter sp. B213. By measuring the growth curve, degradation characteristic and lignolytic enzymes activity of Acinetobacter sp. B213, the culture medium and inoculum size were optimized. The optimal medium was sodium lignosulfonate LB medium and the optimal inoculum size was 8%. After the biological pretreatment of corn straw under the optimized conditions, the cellulose retention rate and lignin degradation rate were 98.51% and 12.02%, respectively. Approximately 10.53% increase in cellulose retention rate and 20.36% decrease in lignin degradation rate was observed compared with the chemical pretreatment (87.98% and 32.38%), respectively. After 24 h hydrolysis, the glucose yield of biological pretreatment corn straw was 100%, which was 14.25% higher than that of the chemical pretreatment. These results indicated that the biological pretreatment with selective lignin degradation has an advantage in the hydrolysis of cellulose and produces glucose, although the lignin degradation efficiency was lower than chemical pretreatment.

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Section: Cellulase Hydrolysis After Pretreatmentmentioning

confidence: 99%

A newly isolated lignin degradation bacterium Acinetobacter sp. B213 pretreatment effectively improved hydrolysis of corn straw by enhance cellulose retention and reduce lignin degradation inhibitor

Zhang

Liu

et al. 2022

Preprint

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“…Therefore, an ideal enzymatic cocktail might have satisfactory concentrations of all these enzymes. Thus, the search for new microbial species producing these enzymes is constant (Zang et al, 2018;Nwamba et al, 2021). A simple and rapid screening method is the evaluation of the fungal growth on petri plates containing microcrystalline cellulose (Avicel) as the sole carbon source and staining with Congo red, which reveals halos of hydrolysis.…”

Section: Influence Of the Enzymatic Synergismmentioning

confidence: 99%

“…Agrawal et al (2021), for example, produced D-feruloyl esterase and β-xylosidase by the fungus Scytalidium thermophilum and observed that the use of these homemade cocktails improved the hydrolysis of rice straw and sugarcane bagasse, demonstrating potential to reduce the load of commercial enzymes, and consequently, the cost of the sugars production from agricultural wastes. The action of accessory enzymes was also evaluated by Nwamba et al (2021), who employed β-glucosidase (Novozymes) and endoxylanase (Qingdao Vland Biotech) in association with cellulolytic cocktail LT4, in the hydrolysis of sugarcane bagasse and released high concentration of glucose (131 g/L). These results showed that the use of accessory enzymes can be an important tool to favor the saccharification and make it economically viable (Nwamba et al, 2021).…”

Section: Influence Of the Enzymatic Synergismmentioning

confidence: 99%

Factors that influence the enzymatic hydrolysis of agricultural wastes for ethanol production: a review

Santos

Pasquini²,

Baffi³

2022

J. Eng. Exact Sci.

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Lignocellulosic biomass, such as agricultural and forestry residues, can be reused and serve as sources of sugars for the production of second-generation ethanol (2G) and other bioproducts. However, these wastes are composed by molecules of difficult degradation, which require steps of pretreatment and enzymatic hydrolysis for their bioconversion into fermentable sugars. At the same time, chemical substances with a potential inhibitory effect on the microbial metabolism can also be produced after the pretreatments and hinder the overall yield of the hydrolytic process. For an efficient and low-cost hydrolysis, homemade enzymes produced from agroindustrial residues, such as sugarcane bagasse, can be employed. However, a set of parameters might be adjusted, such as: kind of pretreatment, enzyme load, solids load, hydrolysis time and use of additives, to improve the yields in free sugars using these onsite enzymatic preparations. In this sense, studies involving the optimization of the conditions of pretreatment and saccharification are essential to increase the bioconversion rate of lignocellulose. These strategies are important for the production of value-added products from these wastes and, consequently, offer a correct and profitable destination to them. Hence, this study presents a review of the main features that influence the enzymatic hydrolysis of agricultural wastes and the yield in reducing sugars for ethanol production.

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“…Hence, it should be an integral part of any study where the process has been established and validated, but is pending scale-up, as displayed by Baral et al [29]. Recent attempts by Nwamba et al [102] to minimize enzyme loadings to 2 mg protein g -1 DM show their committed efforts to maximize sugar release (158 g/L total sugars and 83% glucose yield) from 20% al-AGO pretreated SCB within 72 h using LT4 enzyme in combination with AE and additives. The group has been aggressively working towards improving the techno-economics of the process by incremental changes in sugar yields [77,84,102].…”

Section: Key Insights and Learning'smentioning

confidence: 99%

Emerging trends in high-solids enzymatic saccharification of lignocellulosic feedstocks for developing an efficient and industrially deployable sugar platform

Baral

Kumar

Agrawal

2021

Critical Reviews in Biotechnology

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For the techno-commercial success of any lignocellulosic biorefinery, the cost-effective production of fermentable sugars for the manufacturing of bio-based products is indispensable. High-solids enzymatic saccharification (HSES) is a straightforward approach to developing an industrially deployable sugar platform.Economic incentives such as reduced capital and operational expenditure along with environmental benefits in the form of reduced effluent discharge make this strategy more lucrative for exploitation. However, HSES suffers from the drawback of non-linear and disproportionate sugar yields with increased substrate loadings. To overcome this bottleneck, researchers tend to perform HSES at high enzyme loadings. Nonetheless, the production cost of cellulases is one of the key contributors that impair the entire process economics. This review highlights the relentless efforts made globally to attain a high-titre of sugars and their fermentation products by performing efficient HSES at low cellulase loadings. In this context, technical innovations such as advancements in new pretreatment strategies, next-generation cellulase cocktails, additives, accessory enzymes, novel reactor concepts and enzyme recycling studies are especially showcased. The review further covers new insights, learnings and prospects in the area of lignocellulosic bioprocessing.

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Efficiency enhancement of a new cellulase cocktail at low enzyme loading for high solid digestion of alkali catalyzed atmospheric glycerol organosolvent pre-treated sugarcane bagasse

Cited by 25 publications

References 43 publications

A newly isolated lignin degradation bacterium Acinetobacter sp. B213 pretreatment effectively improved hydrolysis of corn straw by enhance cellulose retention and reduce lignin degradation inhibitor

A newly isolated lignin degradation bacterium Acinetobacter sp. B213 pretreatment effectively improved hydrolysis of corn straw by enhance cellulose retention and reduce lignin degradation inhibitor

Factors that influence the enzymatic hydrolysis of agricultural wastes for ethanol production: a review

Emerging trends in high-solids enzymatic saccharification of lignocellulosic feedstocks for developing an efficient and industrially deployable sugar platform

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