Efficiency Comparison of a Novel E2 Subunit Vaccine and a Classic C-Strain Vaccine against Classical Swine Fever

Zhou, Pei; Huang, Jian; Li, Yanchao; Chen, Hui; Wu, Yidan; Fu, Xueying; Hao, Xiangqi; Li, Qi; Zeng, Rongyu; Zhang, Guihong

doi:10.3390/vetsci8080148

Cited by 3 publications

(2 citation statements)

References 41 publications

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“…MLV have been extensively use in endemic areas, but in recent decades, novel subunit vaccines based on E2 glycoprotein has also become a viable alternative by demonstrating protection against horizontal and vertical transmission and the potential to differentiate vaccinated from infected animals [4,7].…”

Section: Introductionmentioning

confidence: 99%

Stability of closed and needle-punctured vials of Porvac ® subunit vaccine against classical swine fever subjected to thermal stress

Sardina-González,

Vargas-Hernández,

Sordo-Puga

et al. 2024

Preprint

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Background Classical Swine Fever (CSF) is still one of the most economically important viral diseases of pigs. In endemic countries, the disease is controlled mostly through vaccination, hence, the availability of safe and effective vaccines is of utmost importance. Vaccines intended for application in developing countries must also be thermally stable, since the infrastructure needed to maintain a cold chain in those countries is usually lacking. Porvac® is a second-generation subunit marker vaccine against CSF that has demonstrates to be safe and protective. Previous studies have also shown that the vaccine is stable for 1 week at 37 oC and have a shelf life of at least 36 months at 2–8 oC. The aim of this work was to further explore the accelerated stability of Porvac® by assessing the physicochemical properties of the emulsion, and the safety and efficacy of the vaccine subjected to more drastic conditions of thermal stress: (1) 25 oC for 12 months; (2) 30oC and 37 oC for one month and (3) 15 days at 37°C after the cap of the vials had been needle-punctured. Results The vaccine subjected to all these conditions did not show significant changes in the physicochemical properties of the emulsion; did not produce local or systemic adverse reactions in pigs, and the chromatographic profile of the recovered antigen was preserved. All vaccinated swine developed protective neutralizing antibody titers ≥ 1:1000 at 28 days post vaccination. Conclusions Porvac® is stable in all the experimental conditions tested, even after cap puncture, and retains the capacity to induce protective neutralizing antibodies. These results reinforce the robustness of the vaccine, and support its use as a very attractive alternative to modified live vaccines in developing countries endemic for CSF.

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Section: Introductionmentioning

confidence: 99%

Stability of closed and needle-punctured vials of Porvac ® subunit vaccine against classical swine fever subjected to thermal stress

Sardina-González,

Vargas-Hernández,

Sordo-Puga

et al. 2024

Preprint

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show abstract

“…In the 20th century, CSF became endemic in many countries and regions, resulting in significant economic losses to the pig industry due to its high morbidity and mortality [ 4 ]. To prevent CSF, various vaccines have been developed and applied, while C-strain, a live lapinized attenuated vaccine developed in China, is the most widely used [ 5 ]. Alternative vaccines consist of genetically engineered formulations based on the E2 protein of CSFV.…”

Section: Introductionmentioning

confidence: 99%

Ferritin Nanoparticle Delivery of the E2 Protein of Classical Swine Fever Virus Completely Protects Pigs from Lethal Challenge

Zhong,

Lu,

Xia

et al. 2024

Vaccines

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Classical swine fever (CSF), caused by the classical swine fever virus (CSFV), results in significant economic losses to the swine industry in many countries. Vaccination represents the primary strategy to control CSF and the CSFV E2 protein is known as the major protective antigen. However, the E2 protein expressed or presented by different systems elicits distinct immune responses. In this study, we established a stable CHO cell line to express the E2 protein and delivered it using self-assembled ferritin nanoparticles (NPs). Subsequently, we compared the adaptive immune responses induced by the E2-ferritin NPs and the monomeric E2 protein produced by the CHO cells or a baculovirus expression system. The results revealed that the NP-delivered E2 protein elicited higher titers of neutralizing antibodies than did the monomeric E2 protein in pigs. Importantly, only the NP-delivered E2 protein significantly induced CSFV-specific IFN-γ-secreting cells. Furthermore, all the pigs inoculated with the E2-ferritin NPs were completely protected from a lethal CSFV challenge infection. These findings demonstrate the ability of the E2-ferritin NPs to protect pigs against the lethal CSFV challenge by eliciting robust humoral and cellular immune responses.

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Evaluation of an alternative method for determination of Protective Dose50 of Classical swine fever vaccines

et al. 2023

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Efficiency Comparison of a Novel E2 Subunit Vaccine and a Classic C-Strain Vaccine against Classical Swine Fever

Cited by 3 publications

References 41 publications

Stability of closed and needle-punctured vials of Porvac ® subunit vaccine against classical swine fever subjected to thermal stress

Stability of closed and needle-punctured vials of Porvac ® subunit vaccine against classical swine fever subjected to thermal stress

Ferritin Nanoparticle Delivery of the E2 Protein of Classical Swine Fever Virus Completely Protects Pigs from Lethal Challenge

Evaluation of an alternative method for determination of Protective Dose50 of Classical swine fever vaccines

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