Efficiency and cytotoxicity analysis of cationic lipids-mediated gene transfection into AGS gastric cancer cells

Gharaati-Far, Nasrin; Tohidkia, Mohammad Reza; Dehnad, Alireza; Omidi, Yadollah

doi:10.1080/21691401.2017.1355311

Cited by 22 publications

(29 citation statements)

References 32 publications

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“…These fi ndings are consistent with other studies on cytotoxicity effect of Turbofect (8). Although there are many reports about the cellular toxicity of Lipofectamine 2000 in comparison with other transection reagents such as Turbofect, there are just few published studies on toxicity of Lipofectamine 3000 (9,19,(20)(21)(22)(23). In one study, Lipofectamine CRISPRMAX was compared with Lipofectamine 3000 and Lipofectamine RNAiMAX, and signifi cant effi ciency with less toxicity of Lipofectamine CRISPRMAX was shown (12,(23)(24)(25)(26).…”

Section: Discussionsupporting

confidence: 80%

Comparison of transfection efficiency of polymer-based and lipid-based transfection reagents

Rahimi¹,

Mobarakeh²,

Kamalzare³

et al. 2018

BLL

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OBJECTIVES: In this study, the optimal dose of Lipofectamine 3000 and Turbofect to transfect adherent cell lines such as CHO-K1 and HEK293 cells in comparison with non-adherent H9T-cells with pEGFP-N1 and pCDH was identifi ed. BACKGROUND: Lipofectamine 3000 is a new transfection reagent which is claimed to be more effi cient than other transfection reagents like Turbofect. Transfection effi ciency could be affected by the nature of target cell line and vector. METHODS: Transfection effi ciency and cytotoxicity of each reagent was identifi ed by using fl ow cytometry and XTT assay, respectively. RESULTS: Lipofectamine 3000 was more effi cient in transfecting pCDH, while Turbofect was more effi cient in separate transfection of CHO-K1 and HEK293 with pEGFP-N1. Lipofectamine 3000 could be cytotoxic in transfecting H9T-cells with pCDH. Also, H9T-cells were not suffi ciently transfected with each plasmid vector by using each Lipofectamine 3000 and Turbofect. Turbofect had less cytotoxicity effect on all three cell lines than Lipofectamine 3000.Transfection of suspended cells like H9T-cells by using Lipofectamine 3000 and Turbofect would not result in suffi cient transfection. CONCLUSION: Lipofectamine 3000 is the best choice for transfection of CHO-K1 and HEK293 with pCDH while Turbofect is preferably used in transfecting these cell lines with pEGFP-N1 (Tab. 1, Fig. 2, Ref. 26).

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Section: Discussionsupporting

confidence: 80%

Comparison of transfection efficiency of polymer-based and lipid-based transfection reagents

Rahimi¹,

Mobarakeh²,

Kamalzare³

et al. 2018

BLL

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“…It has previously revealed that the prevalence of TAFs was closely correlated with the potential of gastric cancer metastasis [35]. Moreover, TGF-b and FGF-7 produced from fibroblasts could increase gastric cancer cell proliferation and invasiveness [36,37]. Several reports have also demonstrated the TAFs express MCP-1/CCL2 when co-cultured with cancer cells and induce cell survival and immunosuppression [26,38].…”

Section: Discussionmentioning

confidence: 98%

Relationship between the expressions of PD-L1 and tumour-associated fibroblasts in gastric cancer

et al. 2019

Artificial Cells, Nanomedicine, and Biotechnology

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Previous studies have focused on the changes of tumour cells in immune escape, and less is known about the effect of tumour microenvironment (TME) on immune escape. Tumour-associated fibroblasts (TAF) is an important part of the TME and has special physiological and biochemical characteristics, but the specific mechanism has not been clarified. In order to investigate the effect of TAF on the expression of PD-L1 in gastric cancer cells, gastric cancer cell lines MNK45, SGC7901 were non-contact coculturing with TAF 1, 3 and 7 d via transwell. PD-L1 mRNA and protein expression were detected using qRT-PCR and FCM. Then, 95 cases of gastric cancer tissues were selected and evaluated PD-L1 and TAF expressions by immunohistochemical examination. The results showed that the mRNA and protein expression of PD-L1 in the experiment group were significantly higher than that in the control group. PD-L1 expression was associated with massive lymphocyte infiltration, diffuse/mixed histology and intratumoral TAFs in gastric cancers. In conclusion, TAFs promoted the growth in gastric cancer cell lines by increased the PD-L1 expression. ARTICLE HISTORY

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“…An AGS (gastric adenocarcinoma) cell line and XTT method was selected for cytotoxicity studies as described before in literature [13]. Cells were cultured in a DMEM-F12 medium (supplemented with 10% foetal bovine serum, penicilliumstreptomycin) at 37 C with 5% CO 2 .…”

Section: Cytotoxicity Experimentsmentioning

confidence: 99%

Molecular docking of immunogenic peptide ofToxoplasma gondiiand encapsulation with polymer as vaccine candidate

Çakır-Koç

Budama‐Kilinc

Kökcü

et al. 2018

Artificial Cells, Nanomedicine, and Biotechnology

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Toxoplasma gondii is one of the most widely spread parasitic organisms in the world. T. gondii causes primary, chronic infection and mortality. Major surface antigen 1 is the most abundant tachyzoite surface protein and highly conserved between species and causes strong humoural response. Some studies showed that the peptide sequence of surface antigen has immunity. Therefore, tachyzoite surface antigenic peptide sequence is one of the good candidates for vaccine development. However, conformational information and delivery systems are very important parameters for vaccine development. Computational chemistry which is used as an effective method to perform drug or vaccine design provides important information on structure-activity relationship, biological effects of functional groups, molecular geometry, design of enzyme inhibitors and antagonists. The interaction of immunological peptides with protein systems was carried out by means of computing the free energy of binding using the molecular docking technique. Due to the major histocompatibility complex (MHC), proteins play a substantial role for adaptive immunity, the crystal structure of a MHC class I, which plays a pivotal role in the adaptive branch of the immune system, was preferred for docking calculations. A delivery system based on poly(lactic-co-glycolic acid) (PLGA) nanoparticles and peptide loaded PLGA nanoparticles was prepared in this study to improve the bioavailability of tachyzoite surface antigenic peptide sequence. Double emulsion method (water-in-oil-in-water or w/o/w) was used for synthesis of PLGA and peptide loaded PLGA nanoparticles. The average particle size, polydispersity index and zeta potential values of PLGA and peptide loaded PLGA nanoparticles were measured with zeta-sizer by using dynamic light scattering (DLS) technique. The scanning electron microscope (SEM) (Zeiss Supra 50 V) was used for imagining the peptide loaded PLGA nanoparticles. Cell toxicity of nanoparticles was assayed on AGS (gastric adenocarcinoma) cell line. To evaluate mitochondrial activity of cells and toxicity studies, XTT methods were carried out. In this study, we aimed to obtain specific immunological peptide loaded PLGA nanoparticles and characterize the formation with FTIR, zeta sizer and SEM imaging, and evaluate cytotoxicity and carry out molecular docking calculations of peptide-MHC protein in order to enlight in vivo events as vaccine candidate against T. gondii.

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Efficiency and cytotoxicity analysis of cationic lipids-mediated gene transfection into AGS gastric cancer cells

Cited by 22 publications

References 32 publications

Comparison of transfection efficiency of polymer-based and lipid-based transfection reagents

Comparison of transfection efficiency of polymer-based and lipid-based transfection reagents

Relationship between the expressions of PD-L1 and tumour-associated fibroblasts in gastric cancer

Molecular docking of immunogenic peptide ofToxoplasma gondiiand encapsulation with polymer as vaccine candidate

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