Efficiency and Accuracy of PCR-Based Sex Determination Methods in the European Phalacrocoracidae

Thanou, Evanthia; Giokas, Sinos; Goutner, Vassilis; Liordos, Vasilios; Fraguedakis-Tsolis, Stella

doi:10.5735/086.050.0104

Cited by 12 publications

(16 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For molecular sexing of avian feces and degraded tissue materials, considerably shorter fragments (<300 bp) and different priming sites have been targeted to work around this issue (Dawson, Brekke, Dos Remedios, & Horsburgh, ; Dawson et al., ; Faux et al., ). Nevertheless, we chose to work with longer fragments in the present study as the primers have been previously found to be suitable for European cormorants (Thanou et al., ) and cormorant DNA should be contained in intact stomach lining cells. Additionally, detrimental environmental effects on samples such as UV‐radiation and rain (cp.…”

Section: Discussionmentioning

confidence: 99%

“…Nevertheless, we chose to work with longer fragments in the present study as the primers have been previously found to be suitable for European cormorants (Thanou et al, 2013) and cormorant DNA should be contained in intact stomach lining cells. Additionally, detrimental environmental effects on samples such as UV-radiation and rain (cp.…”

Section: Discussionmentioning

confidence: 99%

“…Oehm, Juen, Nagiller, Neuhauser, & Traugott, 2011) were kept minimal by sampling pellets within a few hours after their production. In comparison with studies using the same primers for pure bird samples (Fridolfsson & Ellegren, 1999;Thanou et al, 2013), the herewith applied PCR conditions were more selective (i.e., annealing temperature of 55°C compared to 48-50°C) to avoid the amplification of fish DNA. Also, the presence of inhibitory substances (King et al, 2008) cannot be ruled out entirely even though cormorant pellets were successfully used for molecular identification of consumed fish.…”

Section: Discussionmentioning

confidence: 99%

“…Generally, the technique is based on sequence variations of the sex chromosomes, and depending on the species under investigation, different primers have been developed and applied in allele-specific PCRs (Dawson, Dos Remedios, & Horsburgh, 2016;Morinha, Cabral, & Bastos, 2012). For European cormorants, Thanou, Giokas, Goutner, Liordos, and Fraguedakis-Tsolis (2013) found the primers 2550F and 2718R (Fridolfsson & Ellegren, 1999), targeting the chromodomain-helicase-DNA-binding protein 1 gene (CHD1) to be best suited for molecular sexing. Traditionally, blood, feathers, muscle tissue, egg shells, and buccal swab samples have been used as a source of bird DNA (Griffiths, Daan, & Dijkstra, 1996;Morinha et al, 2013;Thanou et al, 2013), but recently, molecular sexing has also been successfully carried out with fecal samples (e.g., Faux, McInnes, & Jarman, 2014;Jarman et al, 2013).…”

Section: The Great Variety Of Freshwater Habitats In the Central Euromentioning

confidence: 99%

See 3 more Smart Citations

Sex‐specific prey partitioning in breeding piscivorous birds examined via a novel, noninvasive approach

Thalinger

Oehm

Zeisler

et al. 2018

Ecology and Evolution

View full text Add to dashboard Cite

Piscivorous birds frequently display sex‐specific differences in their hunting and feeding behavior, which lead to diverging impacts on prey populations. Cormorants (Phalacrocoracidae), for example, were previously studied to examine dietary differences between the sexes and males were found to consume larger fish in coastal areas during autumn and winter. However, information on prey partitioning during breeding and generally on sex‐specific foraging in inland waters is missing. Here, we assess sex‐specific prey choice of Great Cormorants (Phalacrocorax carbo) during two subsequent breeding seasons in the Central European Alpine foreland, an area characterized by numerous stagnant and flowing waters in close proximity to each other. We developed a unique, noninvasive approach and applied it to regurgitated pellets: molecular cormorant sexing combined with molecular fish identification and fish‐length regression analysis performed on prey hard parts. Altogether, 364 pellets delivered information on both, bird sex, and consumed prey. The sexes differed significantly in their overall prey composition, even though Perca fluviatilis, Rutilus rutilus, and Coregonus spp. represented the main food source for both. Albeit prey composition did not indicate the use of different water bodies by the sexes, male diet was characterized by higher prey diversity within a pellet and the consumption of larger fish. The current findings show that female and male cormorants to some extent target the available prey spectrum at different levels. Finally, the comprehensive and noninvasive approach has great potential for application in studies of other piscivorous bird species.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: The Great Variety Of Freshwater Habitats In the Central Euromentioning

confidence: 99%

See 2 more Smart Citations

Sex‐specific prey partitioning in breeding piscivorous birds examined via a novel, noninvasive approach

Thalinger

Oehm

Zeisler

et al. 2018

Ecology and Evolution

View full text Add to dashboard Cite

show abstract

“…Bird species that display distinct sexual dimorphism do not pose difficulty in identifying their sex, which can be performed based on the size or plumage colour (Thanou et al, 2013). According to Griffiths et al, (1998), over 50% of the bird species exhibit monomorphism in relation to sex, which causes difficulty in determining their sex based on tertiary sexual characteristics.…”

Section: Introductionmentioning

confidence: 99%

Molecular identification of sex in the monomorphic breed of pigeons

Gruszczyńska

Alama

Mi1sko

et al. 2019

IJAR

View full text Add to dashboard Cite

In many avian species, especially in monomorphic species and breeds, sex identification creates a serious problem, as they do not show any phenotypic differences. One of such breeds is the Wroclaw Meat Pigeon. In this study, molecular identification of sex with P2 and P8 primers used for the CHD1 (chromo-helicase-DNA-binding-protein) gene amplification was performed. Peripheral blood samples were analyzed from 46 birds, and their DNA was isolated with the phenol-chloroform method. The fragments (370 bp CHD1-Z; 350 bp CHD1-W) obtained from the PCR were cut with the BsuRI. Only the sequence in the Z chromosome was cut into fragments of 305 and 65 bp by the restriction enzyme. The difference between CHD1-Z and CHD1-W was visualized in 3% agarose gel. A single band was identified as male, whereas two bands (plus 1 invisible) were identified as female. Consequently, 23 specimens in each sex were identified.

show abstract

Genome-wide SNP analysis suggests male heterogamety in bighead catfish (Clarias macrocephalus, )

et al. 2021

View full text Add to dashboard Cite

Efficiency and Accuracy of PCR-Based Sex Determination Methods in the European Phalacrocoracidae

Cited by 12 publications

References 34 publications

Sex‐specific prey partitioning in breeding piscivorous birds examined via a novel, noninvasive approach

Sex‐specific prey partitioning in breeding piscivorous birds examined via a novel, noninvasive approach

Molecular identification of sex in the monomorphic breed of pigeons

Genome-wide SNP analysis suggests male heterogamety in bighead catfish (Clarias macrocephalus, )

Contact Info

Product

Resources

About