Efficacy testing of teat dip solutions used as disinfectants for the dairy industry: Antimicrobial properties

Garvey, Mary; Curran, Damien; Savage, Micheal

doi:10.1111/1471-0307.12344

Cited by 12 publications

(14 citation statements)

References 22 publications

(30 reference statements)

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“…Using the spread plate method, 100 μl of an appropriate dilution was transferred onto Milk Plate Count Agar (Oxoid, Basingstoke, UK) plates, which were incubated at 37°C for 24 h. 22 bacterial isolates displaying the morphology of pathogens associated with mastitis were then taken from each plate and streaked for isolation and purification. Once isolated, these bacterial cultures were Gram stained and bacterial identification was carried out using biochemical tests (catalase and oxidase tests), growth at various temperatures (25°C and 35°C), and on various types of agars [25] and using 16S rDNA sequencing of products generated by polymerase chain reaction (PCR) [21,26].…”

Section: Methodsmentioning

confidence: 99%

“…Twelve commercially available teat disinfectant products, with different active ingredients of varying concentrations, were tested against the isolates identified above. An iodine product was chosen as a positive control, as iodine has been previously shown to effectively inhibit mastitis-related bacteria [11,25]. The disinfectants used were predominately ready-to-use products apart from two products (the control and ammonium lauryl sulfate [product number 6]) which were concentrated products requiring dilution to a usable concentration (Table-1).…”

Section: Methodsmentioning

confidence: 99%

“…To meet the requirements of this standard, the product tested with this standard must demonstrate at least a 10 5 log reduction in viable counts of the test species within 5 min. The disk diffusion assay, on the other hand, has the potential to quantitatively assess the ability of a large range of teat disinfectant products to inhibit bacterial growth [25] at a laboratory scale within a short time period.…”

Section: Introductionmentioning

confidence: 99%

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Screening commercial teat disinfectants against bacteria isolated from bovine milk using disk diffusion

et al. 2019

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Background and Aim: Teat disinfection is an important tool in reducing the incidence of bovine mastitis. Identifying the potential mastitis-causing bacterial species in milk can be the first step in choosing the correct teat disinfectant product. The objective of this study was to screen commercial teat disinfectants for inhibition against mastitis-associated bacteria isolated from various types of milk samples. Materials and Methods: Twelve commercially available teat disinfectant products were tested, against 12 mastitis-associated bacteria strains isolated from bulk tank milk samples and bacterial strains isolated from clinical (n=2) and subclinical (n=3) quarter foremilk samples using the disk diffusion method. Results: There was a significant variation (7-30 mm) in bacterial inhibition between teat disinfection products, with products containing a lactic acid combination (with chlorhexidine or salicylic acid) resulting in the greatest levels of bacterial inhibition against all tested bacteria (p<0.05). Conclusion: In this study, combined ingredients in teat disinfection products had greater levels of bacterial inhibition than when the ingredients were used individually. The disk diffusion assay is a suitable screening method to effectively differentiate the bacterial inhibition of different teat disinfectant products.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Screening commercial teat disinfectants against bacteria isolated from bovine milk using disk diffusion

et al. 2019

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“…Основним завданням молочної промисловості є виробництво сирого молока, стандартизованого за вмістом мікроорганізмів, що визначає його якість під час зберігання. Це можна досягти лише за умови регулярного очищення та дезінфекції доїльного обладнання та молочного інвентаря (Garvey et al, 2017;Wang et al, 2019).…”

Section: вступunclassified

Investigation of the minimum bactericidal concentration of acid detergent “Milkodez” on the test culture of microorganisms

Verkholiuk

2019

SMLNUVMB

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The main task of the dairy industry is the production of raw milk, the quality of which depends on the degree of bacterial background of milk and is achieved only if the regular cleaning and disinfection of milking equipment, dairy equipment. Milk is a good nutrient for microorganisms. The safety of milk that is obtained in dairy complexes provides for acceptable standards in its composition of any chemical, physical or biological substances that pose a potential threat to human health. The largest share of the listed factors belongs to microbiological contamination. The purpose of the work was to investigate the minimum bactericidal concentration of the acid detergent “Milkodez” on the test culture of microorganisms: S. aureus ATCC 25923, E. coli 055K59 No. 3912/41, P. aeruginosa 27/99. The study found that the acid “Milkodez” has bactericidal activity on the S. aureus culture test at dilution of a disinfectant 1: 268.8 and less and an active substance concentration of 0.371% or more at exposure of 2 minutes, for breeding 1:376, 5 and less (concentration of the active substance 0.265% and more) at exposure of 5 minutes and at dilution 1:1466.3 and less and a concentration of 0.0691% or more for 15 minutes. Minimal bactericidal concentration of the acid “Milkodez” on the test of E. coli culture at an exposure of 2 minutes amounted to 0.134%, at an exposure of 5 minutes. – 0.0493% and 15 minutes – 0.0352%. When the concentration of the agent is 0.012856% or more and its dilution is 1:7778.4 and less, throughout all investigated time ranges, there is no growth of the test culture P. aeruginosa. The use of the product at a temperature of 70 ± 5 °C allowed to inhibit the golden staphylococci at the concentration of the solution of the drug 0.371% for 2 minutes of action and 0.265% and 0.0691% – respectively within 5 and 15 minutes. At the same time, at the temperature of the solution of the Milkodez solution 70 ± 5 °C, the death of the E. coli was observed at the concentration of the acidic substance 0.0352%, 0.0493% and 0.134%, respectively, for 2, 5 and 15 minutes. The increase in the temperature of the solution from 20 ± 5 °C to 70 ± 5 °C resulted in the death of P. aeruginosa bacteria at a concentration of 0.01285% in all time ranges.

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“…According to the State Veterinary Pharmacology, Toxicology and Pharmaceutical Science and Phytosanitary Service of Ukraine, as of 2017, 46 trade names for cattle mastitis products were registered on the veterinary drugs market of the country [7]. But, experience shows that the problem of improving dairy herds from mastitis is not always solved by medical measures, they must be supported by a set of organizational, economic, zootechnical, veterinary and sanitary measures aimed at increasing the nonspecific resistance of the organism, timely elimination of causes and factors contributing to the emergence of new cases disease [8].…”

Section: Introductionmentioning

confidence: 99%

Substantiation for the Technology of Obtaining Antimicrobial Spray on the Basis of Silver and Copper Citrates

Polova

Almakayeva

2018

EUREKA: Health Sciences

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Formation and development of the veterinary pharmacy and veterinary drugs market in Ukraine is an urgent issue. In order to prevent infection of the mammary gland in cows after milking, the nipples are treated with a suitable disinfectant for complete immersion or spraying. However, the agents for the prevention of mastitis, presented in the Ukrainian market, are expensive and there is a need to develop domestic veterinary preparations of different forms of release. This work is a continuation of research on the development of the composition and technology of a means for antiseptic treatment of nipples and udders of cattle, in the form of a spray, in order to prevent microbial contamination and prevent mastitis. To obtain a stable veterinary drug based on citrate of silver and copper, the dissolution regimes of the substances were investigated, the order of introduction of the active ingredients and auxiliary substances into the solution, the duration and the rate of stirring during dissolution. It is established that the most optimal are the following technological modes of spray preparation: dissolution temperature 20-220С, mixing time from 10 to 15 minutes, with stirring intensity from 150-200 sec-1. The selected technological regime ensures the stability of the veterinary drug "Argocide-copper" during storage for 12 months. The technology of obtaining a spray in the conditions of industrial production of veterinary preparations of LLC "Brovafarm" (Brovary, Ukraine) is developed. Critical stages and critical control points in the production process of a veterinary preparation of antiseptic action are determined.

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Efficacy testing of teat dip solutions used as disinfectants for the dairy industry: Antimicrobial properties

Cited by 12 publications

References 22 publications

Screening commercial teat disinfectants against bacteria isolated from bovine milk using disk diffusion

Screening commercial teat disinfectants against bacteria isolated from bovine milk using disk diffusion

Investigation of the minimum bactericidal concentration of acid detergent “Milkodez” on the test culture of microorganisms

Substantiation for the Technology of Obtaining Antimicrobial Spray on the Basis of Silver and Copper Citrates

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