Efficacy of various concentrations of NaOCl and instrumentation techniques in reducing <i>Enterococcus faecalis</i> within root canals and dentinal tubules

Berber, Vanessa Bellocchio; Gomes, Brenda Paula Figueiredo de Almeida; Sena, Neylla Teixeira; Vianna, Morgana Eli; Ferraz, Caio Cézar Randi; Zaia, Alexandre Augusto; Souza-Filho, Francisco José de

doi:10.1111/j.1365-2591.2005.01038.x

Cited by 167 publications

(162 citation statements)

References 35 publications

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“…Previous ex vivo studies have shown that manual and conventional rotary preparations have similar effects on microbial populations within straight root canals. 14,20,21 This was corroborated in the present study and might be explained by the ability of the instruments to remove contaminated dentine, ) indicate the presence of significant differences in percent reduction between the groups (p < 0.001). Lower case letters ( a or b ) indicate significant differences between the groups, before and after instrumentation, respectively (p < 0.001).…”

Section: Discussionsupporting

confidence: 77%

Ex vivo evaluation of three instrumentation techniques on E. faecalis biofilm within oval shaped root canals

et al. 2015

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Ex vivo evaluation of three instrumentation techniques on E. faecalis biofilm within oval shaped root canals Abstract: The objective of the present study was to assess the effectiveness of reciprocating instrumentation in disinfecting oval-shaped root canals infected with Enterococcus faecalis. Forty-five human lower premolars were infected with a culture of E. faecalis (ATCC 29212) for 28 days. Five other teeth that were neither contaminated nor instrumented were used as controls. The 45 specimens were divided into three experimental groups (n = 15) based on the root canal preparation technique used: manual (K-type, Dentsply Maillefer, Ballaigues, Switzerland); rotary (MTwo, VDW GmbH, Munich, Germany); and reciprocating (Reciproc R50, VDW GmbH, Munich, Germany) instruments. During chemomechanical preparation, 21 mL of 2.5% NaOCl was used as the irrigating solution. Microbiological sampling was performed before (S1) and immediately after (S2) the chemomechanical preparation using sterilized paper points. Specimens were then cleaved, and 0.02 g of dentine chips was collected from the root thirds to verify the presence of microorganisms in dentinal tubules. All three preparation techniques reduced the number of microorganisms in the root canal lumen and dentine chips from the root thirds, but no significant differences were observed between the three groups (p > 0.05). Reciprocating instrumentation with Reciproc R50 was effective in reducing the number of microorganisms within the root canal system. Although this technique involves the use of only one file to perform the root canal therapy, it is as effective as conventional rotary instrumentation in reducing the E. faecalis biofilm from the root canal system. However, further clinical investigations are warranted in order to ratify these results.

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Section: Discussionsupporting

confidence: 77%

Ex vivo evaluation of three instrumentation techniques on E. faecalis biofilm within oval shaped root canals

et al. 2015

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“…The removal of the apical dentin during biomechanical preparation plays an important role in the cleaning and disinfection of the root canal system. According to Berber et al, 26 the microorganisms inside the root canal are able to penetrate the dentinal tubules at around 200 micrometers. Regarding the impact of instrumentation cleaning, Fornari et al 27 observed that the larger the final diameter, the greater the percentage of touched walls, which leads to increased cleaning of the root canal.…”

Section: Discussionmentioning

confidence: 99%

“…Aside from this, the enlargement of the apical segment favors the effectiveness of substances used during both the root canal irrigation as medications in certain periapical pathological conditions. 26,27 In light of the recent efforts to simplify the biomechanical preparation techniques, the effect of rotary instruments at the apical segment should be considered for the proper cleaning, shaping, and disinfection of root canals. Thus, in systems like the Twisted File and ProTaper, which favor the preparation of cervical and middle segments through the use of instruments with greater taper, additional smaller taper files could be considered to complement this technique and enlarge the apical region.…”

Section: Discussionmentioning

confidence: 99%

Computed tomography evaluation of rotary systems on the root canal transportation and centering ability

et al. 2015

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The endodontic preparation of curved and narrow root canals is challenging, with a tendency for the prepared canal to deviate away from its natural axis. The aim of this study was to evaluate, by cone-beam computed tomography, the transportation and centering ability of curved mesiobuccal canals in maxillary molars after biomechanical preparation with different nickel-titanium (NiTi) rotary systems. Forty teeth with angles of curvature ranging from 20° to 40° and radii between 5.0 mm and 10.0 mm were selected and assigned into four groups (n = 10), according to the biomechanical preparative system used: Hero 642 (HR), Liberator (LB), ProTaper (PT), and Twisted File (TF). The specimens were inserted into an acrylic device and scanned with computed tomography prior to, and following, instrumentation at 3, 6 and 9 mm from the root apex. The canal degree of transportation and centering ability were calculated and analyzed using one-way ANOVA and Tukey's tests (α = 0.05). The results demonstrated no significant difference (p > 0.05) in shaping ability among the rotary systems. The mean canal transportation was: -0.049 ± 0.083 mm (HR); -0.004 ± 0.044 mm (LB); -0.003 ± 0.064 mm (PT); -0.021 ± 0.064 mm (TF). The mean canal centering ability was: -0.093 ± 0.147 mm (HR); -0.001 ± 0.100 mm (LB); -0.002 ± 0.134 mm (PT); -0.033 ± 0.133 mm (TF). Also, there was no significant difference among the root segments (p > 0.05). It was concluded that the Hero 642, Liberator, ProTaper, and Twisted File rotary systems could be safely used in curved canal instrumentation, resulting in satisfactory preservation of the original canal shape.

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“…Several clinical studies 12,25 have shown that even at high concentration of 5%, nearly one third to one half of the root canals remain contaminated after instrumentation and irrigation with NaOCl. Berber et al 26 studied the efficacy of various concentrations of NaOCl and instrumentation techniques in reducing E faecalis within root canals and dentinal tubules. They found no differences among concentrations in cleaning the root canals, but only at the higher concentrations was NaOCl able to disinfect the dentinal tubules independent of the canal preparation technique used.…”

mentioning

confidence: 99%

The antimicrobial effects of root canal irrigation and medication

Karim

Kennedy

Hussey

2007

Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, an

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Efficacy of various concentrations of NaOCl and instrumentation techniques in reducing Enterococcus faecalis within root canals and dentinal tubules

Abstract: Especially at higher concentrations, NaOCl, was able to disinfect the dentinal tubules, independent of the canal preparation technique used.

Cited by 167 publications

References 35 publications

Ex vivo evaluation of three instrumentation techniques on E. faecalis biofilm within oval shaped root canals

Ex vivo evaluation of three instrumentation techniques on E. faecalis biofilm within oval shaped root canals

Computed tomography evaluation of rotary systems on the root canal transportation and centering ability

The antimicrobial effects of root canal irrigation and medication

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