Efficacy of tyrosine kinase inhibitors examined by a combination of Raman micro-spectroscopy and a deep wavelet scattering-based multivariate analysis framework

Abstract

HER2 is a crucial therapeutic target in breast cancer, and the survival rate of breast cancer patients has increased because of this receptor's inhibition. However, tumors showed resistance to this...

Innovative Approaches for Drug Discovery: Quantifying Drug Distribution and Response with Raman Imaging

“…Later, they employed RS microscopy in combination with a deep wavelet scattering-based multivariate analysis framework to evaluate the potency, effectiveness, and distribution of lapatinib and neratinib in breast cancer cells. 147 This approach discriminated between control cells and drug-treated cells with high accuracy, demonstrating significant changes in the spectra of drug-treated cells, particularly in regions associated with lipids, proteins, and nucleic acids. Furthermore, colocalization analysis of RS spectra combined with fluorescence imaging revealed the distribution of lapatinib that binds to EGFR and HER2 receptors.…”

“…By identification of differences in Raman spectra before and after drug treatment, large spectral changes were detected that indicated alterations in cellular composition. Later, they employed RS microscopy in combination with a deep wavelet scattering-based multivariate analysis framework to evaluate the potency, effectiveness, and distribution of lapatinib and neratinib in breast cancer cells . This approach discriminated between control cells and drug-treated cells with high accuracy, demonstrating significant changes in the spectra of drug-treated cells, particularly in regions associated with lipids, proteins, and nucleic acids.…”