Efficacy of Triple Antibiotic Paste,  Moxifloxacin, Calcium Hydroxide And  2% Chlorhexidine Gel In Elimination of  E.  Faecalis : An In vitro Study

Lakhani, Ashik Ali; Sekhar, Kothamasu Soma; Gupta, Pankaj; Tejolatha, Bellam; Gupta, Anjali; Kashyap, Shruti; Desai, Veena; Farista, Shanin

doi:10.7860/jcdr/2017/22394.9132

Cited by 25 publications

(25 citation statements)

References 24 publications

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“…While the results of this study were in disagreement with (Ghabraei et al 2018a) who found that a paste of Ca(OH) 2 mixed with 2% CHX was able to eradicate the E. faecalis biofilm in three days, while TAP was able to eradicate the biofilm of E. faecalis in seven days and were in disagreement with the results of (Lakhani et al 2017) who found that the 2% CHX gel was the most effective medicament against E. faecalis, among the tested medicaments which were normal saline, calcium hydroxide, moxifloxacin or triple antibiotic paste at the end of first, 7 th and 10 th day.…”

Section: Discussioncontrasting

confidence: 99%

Comparison of the Antibacterial Efficacy of Ibuprofen Versus Triple Antibiotic Paste Against Enterococcus Faecalis in Single Rooted Teeth

Rezk

El-Baz

Naeem

2021

Egyptian Dental Journal

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Aim:The aim of the present study was to compare the antibacterial efficiency of the antiinflammatory non-antibiotic ibuprofen versus triple antibiotic paste as an intra-canal medication against Enterococcus faecalis. Methodology: Forty four single rooted teeth were decoronated and instrumented up to F4 ProTaper rotary files. Apical foramen was sealed with acrylic resin, and all the external surfaces were made impermeable with nail varnish, except for the coronal access. Roots were autoclaved at 121°C for 20 minutes, placed in Eppendorf tubes and contaminated with E. faecalis for 14 days. Teeth were divided into two groups of 22 teeth; (Group1: ibuprofen, Group2: triple antibiotic paste (TAP). Colony forming unit (CFU) counts were taken before placing the intracanal medications by a paper point sampling (CFU1). The tested medicaments were mixed with distilled water (1:1 w/v), placed inside the root canals, then canals were temporarily sealed, and incubated at 37°C for 7 days. After intracanal medication removal, the second count (CFU2) was taken as (CFU1) and the antibacterial action of each medication was determined by calculating the percentage of bacterial reduction when comparing CFU2 to CFU1, and then the antibacterial action of the tested medications was compared with each other.Results: There was no statistically significant difference between (T.A.P) and (Ibuprofen) groups in the percentage of bacterial reduction where (p=0.073). Both medications caused a reduction in the bacterial count from CFU1 to CFU2. Conclusion:Ibuprofen proved to have an antibacterial effect comparable to that of TAP.

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Section: Discussioncontrasting

confidence: 99%

Comparison of the Antibacterial Efficacy of Ibuprofen Versus Triple Antibiotic Paste Against Enterococcus Faecalis in Single Rooted Teeth

Rezk

El-Baz

Naeem

2021

Egyptian Dental Journal

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“…Calcium hydroxide and CHX gel are known as the most effective intracanal medicaments and commonly used in dental practices (Haapasalo and Orstavik, 1987; Evans et al, 2003; Lakhani et al, 2017). However, they are not sufficient for the complete inactivation of E. faecalis from root canals in all cases (Siqueira, 2001; Evans et al, 2003; Mozayeni et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

Nematode-Associated Bacteria: Production of Antimicrobial Agent as a Presumptive Nominee for Curing Endodontic Infections Caused by Enterococcus faecalis

et al. 2019

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Xenorhabdus and/or Photorhabdus bacteria produce antibacterial metabolites to protect insect cadavers against food competitors allowing them to survive in nature with their nematode host. The effects of culture supernatant produced by Xenorhabdus and Photorhabdus spp. were investigated against the multidrug-resistant dental root canal pathogen Enterococcus faecalis. The efficacy of seven different cell-free supernatants of Xenorhabdus and Photorhabdus species against E. faecalis was assessed with overlay bioassay and serial dilution techniques. Additionally, time-dependent inactivation of supernatant was evaluated. Among the seven different bacterial species, X. cabanillasii produced the strongest antibacterial effects. Loss of bioactivity in a phosphopantetheinyl transferase-deficient mutant of X. cabanillasii indicated that this activity is likely based on non-ribosomal peptide synthetases (NRPSs) or polyketide synthases (PKSs). Subsequent in silico analysis revealed multiple possible biosynthetic gene clusters (BGCs) in the genome of X. cabanillasii including a BGC homologous to that of zeamine/fabclavine biosynthesis. Fabclavines are NRPS-derived hexapeptides, which are connected by PKS-derived malonate units to an unusual polyamine, also PKS-derived. Due to the known broad-spectrum bioactivity of the fabclavines, we generated a promoter exchange mutant in front of the fabclavine-like BGC. This leads to over-expression by induction or a knock-out by non-induction which resulted in a bioactive and non-bioactive mutant. Furthermore, MS and MS2 experiments confirmed that X. cabanillasii produces the same derivatives as X. budapestensis. The medicament potential of 10-fold concentrated supernatant of induced fcl promoter exchanged X. cabanillasii was also assessed in dental root canals. Calcium hydroxide paste, or chlorhexidine gel, or fabclavine-rich supernatant was applied to root canals. Fabclavine-rich supernatant exhibited the highest inactivation efficacy of ≥3 log10 steps CFU reduction, followed by calcium hydroxide paste (≤2 log10 step). The mean percentage of E. faecalis-free dental root canals after treatment was 63.6, 45.5, and 18.2% for fabclavine, calcium hydroxide, and chlorhexidine, respectively. Fabclavine in liquid form or preferably as a paste or gel formulation is a promising alternative intracanal medicament.

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“…However, Manzur et al found that calcium hydroxide had a limited efficacy in eliminating root canal bacteria because the initially high pH was buffered, and bacteria which could survive in the root canal system at pH 9–10 could not be destroyed [41]. Lakhani et al pointed out that Enterococcus faecalis was tolerant of calcium hydroxide with high pH and resided in the deeper part of the dentin tubule, which was most commonly associated with failed RCT [44]. In the clinical application, Peters et al found that calcium hydroxide, applied as an intracanal disinfectant for 4 weeks, did not further decrease the number of bacteria [45].…”

Section: Discussionmentioning

confidence: 99%

The Clinical Effectiveness of Calcium Hydroxide in Root Canal Disinfection of Primary Teeth: A Meta-Analysis

et al. 2019

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Background The aim of this research was to systematically analyze the effectiveness of calcium hydroxide compared to formocresol (FC) and camphor phenol (CP) in root canal disinfection of primary teeth. Material/Methods The meta-analysis was based on the participants, interventions, control, outcome (PICO) study design principle and 16 randomized-controlled clinical trials published from January 2000 to August 2018. The data heterogeneity of each study was assessed by the Q-test. The odds ratio and 95% confidence interval (CI) were calculated based on the heterogeneity results by Revman software. Results Sixteen randomized-controlled clinical trials of 3047 primary teeth were included in this meta-analysis. There were significant differences of clinical effectiveness between calcium hydroxide and FC in root canal disinfection of primary teeth (OR=3.37; 95% CI range: 2.54–4.48, P <0.01) and endodontic inter-appointment emergencies (EIAE) after disinfection for 7 days (OR=0.26; 95% CI range: 0.16–0.42, P <0.01). However, there was no statistical difference of EIAE, after disinfection of primary teeth for 48 hours, between calcium hydroxide and FC (OR=0.62; 95% CI range: 0.34–1.11, P =0.11). There were significant differences of clinical effectiveness between the calcium hydroxide and CP in root canal disinfection of primary teeth (OR=5.50; 95% CI range: 3.36–8.98, P <0.01). Conclusions This meta-analysis indicated that the effectiveness of calcium hydroxide as root canal disinfectant in primary teeth was more effective than that of FC and CP.

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Efficacy of Triple Antibiotic Paste, Moxifloxacin, Calcium Hydroxide And 2% Chlorhexidine Gel In Elimination of E. Faecalis : An In vitro Study

Cited by 25 publications

References 24 publications

Comparison of the Antibacterial Efficacy of Ibuprofen Versus Triple Antibiotic Paste Against Enterococcus Faecalis in Single Rooted Teeth

Comparison of the Antibacterial Efficacy of Ibuprofen Versus Triple Antibiotic Paste Against Enterococcus Faecalis in Single Rooted Teeth

Nematode-Associated Bacteria: Production of Antimicrobial Agent as a Presumptive Nominee for Curing Endodontic Infections Caused by Enterococcus faecalis

The Clinical Effectiveness of Calcium Hydroxide in Root Canal Disinfection of Primary Teeth: A Meta-Analysis

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