Efficacy of tigecycline and vancomycin in experimental catheter-related Staphylococcus epidermidis infection: microbiological and electron microscopic analysis of biofilm

Aybar, Yesim; Özaras, Reşat; Beşirli, Kazım; Engin, Ersoy; Karabulut, E.; Salihoğlu, Tamer; Mete, Bilgül; Tabak, Fehmı; Mert, Ali; Tahan, Gülgün; Yılmaz, Murat; Öztürk, Recep

doi:10.1016/j.ijantimicag.2012.01.001

Cited by 22 publications

(16 citation statements)

References 41 publications

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“…Intriguingly, there is evidence demonstrating a biofilm-inducing effect of sub-inhibitory vancomycin concentrations on S. epidermidis [29,41], potentially contributing to the failure of antimicrobial therapies in deviceassociated infections. Under circumstances in which vancomycin is prohibited due to nephrotoxic side effects or hypersensitivity, linezolid, daptomycin or tigecycline are potential therapeutic alternatives [42][43][44]. So far, however, potential adverse effects related to biofilm inducing properties of these compounds have only rarely been analysed so far [29].…”

Section: Resultsmentioning

confidence: 99%

Sub-inhibitory tigecycline concentrations induce extracellular matrix binding protein Embp dependent Staphylococcus epidermidis biofilm formation and immune evasion

Weiser

Henke

Hector

et al. 2016

International Journal of Medical Microbiology

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Section: Resultsmentioning

confidence: 99%

Sub-inhibitory tigecycline concentrations induce extracellular matrix binding protein Embp dependent Staphylococcus epidermidis biofilm formation and immune evasion

Weiser

Henke

Hector

et al. 2016

International Journal of Medical Microbiology

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“…Indeed, vancomycin was tested in different animal models of device-related infections and failed to eradicate the biofilm [27][28][29]. The concentration of vancomycin detected in the biofilms was above the MIC values [29], but only cellular structures were destroyed and matrix was left [28]. The remaining material after antibiotic treatment could function as anchorage for other bacteria and encourage re-growth of the biofilm and re-colonisation of the device [6].…”

Section: Discussionmentioning

confidence: 99%

Inefficacy of vancomycin and teicoplanin in eradicating and killing Staphylococcus epidermidis biofilms in vitro

Claessens¹,

Roriz²,

Merckx³

et al. 2015

International Journal of Antimicrobial Agents

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Biofilm-associated bacteria display a decreased susceptibility towards antibiotics. Routine assessment of antibiotic susceptibility of planktonic bacteria therefore offers an insufficient prediction of the biofilm response. In this study, in vitro biofilms of eight clinical Staphylococcus epidermidis strains were subjected to treatment with vancomycin, teicoplanin, oxacillin, rifampicin and gentamicin. In addition, the biofilms were subjected to combinations of an antibiotic with rifampicin. The effects on the biofilms were assessed by crystal violet staining to determine the total biofilm biomass, staining with XTT to determine bacterial cell viability, and microscopy. Combining these methods showed that treatment of S. epidermidis biofilms with glycopeptides increased the total biofilm biomass and that these antibiotics were not effective in killing bacteria embedded in biofilms. The decreased killing efficacy was more pronounced in biofilms produced by strains that were classified as 'strong' biofilm producers. Rifampicin, oxacillin and gentamicin effectively killed biofilm-associated bacteria of all tested strains. Combining antibiotics with rifampicin increased the killing efficacy without influencing the total biofilm biomass. When vancomycin or teicoplanin were combined with rifampicin, the increase in biofilm biomass was neutralised and also the killing efficacy was influenced in a positive way. We conclude that the combined methodology used in this study showed that glycopeptides were not effective in eradicating S. epidermidis biofilms but that combination with rifampicin improved the killing efficacy in vitro.

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“…In CoNS-infected polyurethane catheters inserted in rabbits, electron microscopy showed that Tigecycline was superior to vancomycin in reducing bacterial growth [39].…”

Section: Tigecycline Lock Therapymentioning

confidence: 99%

Antimicrobial lock therapy in central-line associated bloodstream infections: a systematic review

Vassallo

Dunais

Roger³

2015

Infection

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Efficacy of tigecycline and vancomycin in experimental catheter-related Staphylococcus epidermidis infection: microbiological and electron microscopic analysis of biofilm

Cited by 22 publications

References 41 publications

Sub-inhibitory tigecycline concentrations induce extracellular matrix binding protein Embp dependent Staphylococcus epidermidis biofilm formation and immune evasion

Sub-inhibitory tigecycline concentrations induce extracellular matrix binding protein Embp dependent Staphylococcus epidermidis biofilm formation and immune evasion

Inefficacy of vancomycin and teicoplanin in eradicating and killing Staphylococcus epidermidis biofilms in vitro

Antimicrobial lock therapy in central-line associated bloodstream infections: a systematic review

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