Efficacy of three different cervical dislocation methods for on-farm killing of layer chicks

Bandara, R. M. A. S.; Torrey, Stephanie; Turner, Patricia V.; Linden, Alex zur; Schwean-Lardner, K.; Widowski, Tina M.

doi:10.1016/j.psj.2023.102538

Cited by 1 publication

(1 citation statement)

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“…All procedures involving the care and use of animals were conducted to minimize animal suffering and approved by the Jiangsu Laboratory Animal Welfare and Ethics guidelines of the Jiangsu Administrative Committee for Laboratory Animals (SCXK2018-0009 and SYXK 2021-0027 for chickens and SCXK2017-0007 and SYXK2017-0044 for mice). The mice and chickens used in this study were both euthanized by manual cervical dislocation [13,28]. The SPF broilers and chicken embryos used in this study were purchased from Nanjing Biology Medical Factory, Qian Yuan-hao Biological Co., Ltd., Nanjing, China.…”

Section: Ethical Statementmentioning

confidence: 99%

Salmonella typhimurium Vaccine Candidate Delivering Infectious Bronchitis Virus S1 Protein to Induce Protection

Liu,

Li,

et al. 2024

Biomolecules

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Infectious bronchitis (IB) is a highly infectious viral disease of chickens which causes significant economic losses in the poultry industry worldwide. An effective vaccine against IB is urgently needed to provide both biosafety and high-efficiency immune protection. In this study, the S1 protein of the infectious bronchitis virus was delivered by a recombinant attenuated Salmonella typhimurium vector to form the vaccine candidate χ11246(pYA4545-S1). S. typhimurium χ11246 carried a sifA− mutation with regulated delayed systems, striking a balance between host safety and immunogenicity. Here, we demonstrated that S1 protein is highly expressed in HD11 cells. Immunization with χ11246(pYA4545-S1) induced the production of antibody and cytokine, leading to an effective immune response against IB. Oral immunization with χ11246(pYA4545-S1) provided 72%, 56%, and 56% protection in the lacrimal gland, trachea, and cloaca against infectious bronchitis virus infection, respectively. Furthermore, it significantly reduced histopathological lesions in chickens. Together, this study provides a new idea for the prevention of IB.

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Section: Ethical Statementmentioning

confidence: 99%