Efficacy of Thidiazuron in &lt;i&gt;In Vitro&lt;/i&gt; Propagation of Carnation Shoot Tips: Influence of Dose and Duration of Exposure

Onamu, Rose; Obukosia, Silas D.; Musembi, Nicodemus Ndawa; Hutchinson, M. J.

doi:10.4314/acsj.v11i2.27525

Cited by 10 publications

(5 citation statements)

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“…TDZ (Thidiazuron) is a substituted phenyl urea [1-phenyl-3-(1,2,3-thidiazol-5-yl)urea] that has immense potential as a cytokinin in shoot organogenesis in a large number of plant systems including legumes (Murthy et al, 1998;Shan et al, 2000;Ganeshan et al, 2003;Onamu et al, 2003;Mroginski et al, 2004;Sharma et al, 2005;Chen and Chang, 2006;Radhika et al, 2006). Since, the observation made by Malik and Saxena (1992) TDZ has been noted to be the most promising cytokinin for shoot induction in chickpea by number of research teams (Barna and Wakhlu, 1993;Huetteman and Preece, 1993;Murthy et al, 1996;Rizvi and Singh, 2000;Jayanand et al, 2003;Senthil et al, 2004;TewariSingh et al, 2004;Kiran et al, 2005).…”

Section: Resultsmentioning

confidence: 99%

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An optimal protocol for in vitro regeneration, efficient rooting and stable transplantation of chickpea

Anwar

Sharmila

Saradhi

2008

Physiol Mol Biol Plants

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A rapid, reproducible and efficient regeneration method was developed for chickpea (Cicer arietinum L.) using single cotyledon with half embryonal axis as explants. MS medium supplemented with 4 ìM TDZ, 10 ìM 2-iP and 2 ìM kinetin induced 50-100 adventitious buds/shoots after 14 days of culture and elongated on MS medium supplemented with 5 ìM 2-iP and 2 ìM kinetin. Healthy, strong and 100 % rooting was achieved by exposing cut ends of the shoots to 10 sec pulse treatment with 100 ìmoles/ml IBA followed by their transfer to liquid MS basal medium within 10-14 d. 2-3 cm long shoots were most suitable for rooting. Potting-mixture with good aeration and lesser capacity to retain water was most suitable for achieving successful establishment of chickpea plantlets. Garden soil mixed with sand (gravel) and bio-manure in the ratio of 1:1:1 is most suitable for achieving cent percent transplantation success. Cent percent of plantlets got acclimatized, survived in the pots and showed normal growth, development, flowering followed by podding and seeds setting. Harvesting of seeds was done after the pods were fully matured and dry. In this communication, we have demonstrated for the first time that shoot length, pulse treatment of cut ends of shoots with 100 ìmoles/ml IBA and aeration of potting mixture are key factors for rapid micro-propagation and successful establishment of chickpea.

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Section: Resultsmentioning

confidence: 99%

“…Despite its high efficiency to enhance shoot induction, use of TDZ has not become popular due to its immense toxicity resulting in the formation of deformed shoots, whenever it is used above threshold levels and/or for prolonged duration (Devlin et al, 1989;Onamu et al, 2003;Sharma et al, 2005).…”

Section: Resultsmentioning

confidence: 99%

An optimal protocol for in vitro regeneration, efficient rooting and stable transplantation of chickpea

Anwar

Sharmila

Saradhi

2008

Physiol Mol Biol Plants

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“…Fully grown plantlets were sub-cultured for maintenance in growth chamber in controlled conditions (Figure 4, Table 10). Mangal et al [21], and Onamu et al [22], used MS medium supplemented with combination of NAA and Kinetin for shoot induction from meristem. Liquid MS medium was found to be more effective for efficient shoot multiplication than MS medium solidified with agar or phytagel [2].…”

Section: Regeneration and Shoot Multiplication From Morphogenic Callusmentioning

confidence: 99%

High Frequency Plant Regeneration from Leaf Derived Callus of <i>Dianthus caryophyllus</i> L.

Arif¹,

Rauf²,

Din³

et al. 2014

AJPS

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An efficient procedure was developed for in vitro callus induction, proliferation and regeneration of carnation cultivar (Dianthus caryophyllus L.) using leaf, nodal and inter-nodal explants on Murashige and Skoog's medium (MS) supplemented with exogenous plant growth regulators. For morphogenic callus induction and proliferation from various explants, MS medium supplemented with 3.0 mg/l 2,4-D was highly efficient with 100% callus induction frequency from inter-nodal explants. Leaf explants showed quicker response than nodal and inter-modal explants, for callus initiation within 6 days of inoculation. Best grown callus was obtained from leaf explant. The leaf-derived callus was maintained up to several weeks, which indicated that 8-week incubation period was the most suitable for obtaining well proliferated, morphogenic callus. Temperature variation also affected the growth of in vitro induced morphogenic callus from various explants. Results have shown that 27˚C proved to be the best temperature for morphogenic callus induction and proliferation from leaf and inter-nodal explants. Among the auxin-cytokinin combination, MS medium containing 1.0 mg/l N(6)-benzylaminopurin (BAP) and 2.0 mg/l NAA showed the highest efficiency of callus initiation and proliferation from leaf, nodal and inter-nodal explants. Light conditions proved better for callogenesis and proliferation from leaf, nodal and inter-nodal explants. Regeneration response from well grown morphogenic callus was prominent on MS medium supplemented with 3.0 mg/l BAP alone and 1.0 mg/l NAA with 3.0 mg/l BAP.

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“…Among the different cytokinins used in this study, a higher frequency of adventitious shoot buds/multiple shoots formation was observed in TDZ containing medium (Anwar et al, 2008). TDZ induces high frequency of somatic embryogenesis/ adventitious bud formation in some plant species, either alone or in combination with other growth regulator/s (Murthy et al, 1998(Murthy et al, , 1995Chen and Chang, 2006;Delvin, 1989;Ganeshan et al 2003;Huetteman and Preece, 1993;Onamu, 2003;Mroginski, 2004;Shan, 2000;Sharma, 2005;Newton, 2005, 2006 and reference cited in). A substituted phenyl urea derivative was found to be very effective for induction of multiple shoots from nodal and basal regions of the primary epicotyl (Malik and Saxena, 1992).…”

Section: Direct Shoot Organogenesis From Different Explants Of Chickpeamentioning

confidence: 66%

No more recalcitrant: Chickpea regeneration and genetic transformation

Anwar¹,

Sharmila²,

Saradhi³

2010

Afr. J. Biotechnol.

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Chickpea production is limited worldwide because of abiotic and biotic stresses. Efforts to overcome these production constraints through traditional breeding are difficult due to limited genetic variation. Novel regeneration is pre-requisite for genetic transformation offers the opportunity to overcome hybridization barriers and introduce novel genes for resistance. Although direct gene transfer via direct DNA transfer has been reported, Agrobacterium mediated transformation is the preferred method and standard protocols have been established for the production of transgenic plantlets derived from cocultivation of embryonic axes. This was soon adopted due to difficulties associated with regeneration of whole plants from callus. Only few reports have been reported using genetic transformation/ transgene(s) against abiotic stress tolerance transgenic chickpea plants. Transgenic chickpea using bacterial codA gene tolerance against abiotic stresses have been developed. Chickpea improvement and application of genomics tools to the study of the chickpea genome will be enhanced through the use of genetic transformation.

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Efficacy of Thidiazuron in <i>In Vitro</i> Propagation of Carnation Shoot Tips: Influence of Dose and Duration of Exposure

Cited by 10 publications

References 0 publications

An optimal protocol for in vitro regeneration, efficient rooting and stable transplantation of chickpea

An optimal protocol for in vitro regeneration, efficient rooting and stable transplantation of chickpea

High Frequency Plant Regeneration from Leaf Derived Callus of <i>Dianthus caryophyllus</i> L.

No more recalcitrant: Chickpea regeneration and genetic transformation

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Efficacy of Thidiazuron in <i>In Vitro</i> Propagation of Carnation Shoot Tips: Influence of Dose and Duration of Exposure

Cited by 10 publications

References 0 publications

An optimal protocol for in vitro regeneration, efficient rooting and stable transplantation of chickpea

An optimal protocol for in vitro regeneration, efficient rooting and stable transplantation of chickpea

High Frequency Plant Regeneration from Leaf Derived Callus of &lt;i&gt;Dianthus caryophyllus&lt;/i&gt; L.

No more recalcitrant: Chickpea regeneration and genetic transformation

Contact Info

Product

Resources

About

High Frequency Plant Regeneration from Leaf Derived Callus of <i>Dianthus caryophyllus</i> L.