Efficacy of the Latham Blood Processor To Perform Plateletpheresis

Greater numbers of platelets are recovered during discontinuous flow centrifugation than can be simply accounted for by the decrease in total circulating platelets in the donor. There is a linear relationship between the logarithm of the circulating platelet count and the number of plateletpheresis bowls filled. The disappearance of platelets from the peripheral circulation occurs at a greater rate in splenectomized donors than in normal donors, and the rate of platelet disappearance in normal donors is less than what would be expected if there were no in vivo platelet storage pools. The data suggest the redistribution of platelets from the spleen in normal donors during the time course of the procedure.

Section: Introductionmentioning

confidence: 99%

Redistribution of Platelets During Discontinous Flow Platelet Pheresis

Katz¹,

Reiss²,

Houx³

1978

“…The platelets collected with the Fenwal CS-3000 had low leukocyte counts; leukocytepoor platelets are recommended for patients requiring repeated platelet transfusions [1,4], It should be noted that the IBM Blood Processor now can be operated with a dual stage channel to collect a leukocyte-poor platelet concentrate, with a platelet yield comparable to that achieved with the Fen wal CS-3000 [6], During plateletpheresis, platelets are mo bilized, probably from the splenic storage pool, into the vascular space [7][8][9][10], An un expected finding from this study was that donors plateletpheresed with the IBM Blood Processor 2997 (31%) exhibited a signifi cantly lower platelet count after collection than donors plateletpheresed with the Haemonetics Blood Processor 30 (18%) or the Fenwal CS-3000 (19%). The number of platelets isolated with the IBM Blood Pro cessor 2997 was similar to the Haemonetics Blood Processor 30, and the IBM Blood Pro cessor 2997 and the Fenwal CS-3000 had similar collection times.…”

Section: Resultsmentioning

confidence: 99%

Plateletpheresis Experience with the Haemonetics Blood Processor 30, the IBM Blood Processor 2997, and the Fenwal CS-3000 Blood Processor

Kurtz¹,

McMican²,

Carciero³

et al. 1981

Comparisons were made of the apheresis instruments Haemonetics Blood Processor 30, IBM Blood Processor 2997 and Fenwal CS-3000, for collection of platelets from normal donors. With each instrument the mean recovery was at least 4x10^11 platelets per procedure, and each instrument afforded a safe and reliable collection. The Haemonetics Blood Processor gave the lowest recovery of platelets per minute per procedure. The IBM Blood Processor 2997 required the longest time for set-up and priming and processed 1.5 liters more donor blood per collection than the other instruments. The Fenwal CS-3000, which is a computer-controlled instrument, was the least time consuming. The donor suffered a significantly greater drop in platelet count after collection with the IBM Blood Processor 2997 (31%) than after collection with the other instruments (19%), and we were unable to account for this observation.

“…Although it has not been shown that infu sion of HES into healthy blood donors re pheresis [21], and probably reflect changes in blood volume caused by the supine position [24], infusion of intravenous fluids and phys ical removal of platelets. The peripheral WBC count increased significantly as a re sult of the procedure (table I).…”

Section: Discussionmentioning

confidence: 99%

“…Calculations Liters of blood processed (LBP) was calculated with the following formula [21]: WBC Harvesting The contents of bag No. 1 (HES and cells) and of the bowl (WBC-rich RBC) were directed into bag No.…”

Section: Laboratory Testsmentioning

confidence: 99%

A New Method to Collect Granulocytes Using a Low Dose of Hydroxyethyl Starch

Szymanski¹

1983

A new granulocyte collection method employing discontinuous flow centrifugation and low donor dose of hydroxyethyl starch (HES) is presented. Following the procedure, the increase in serum polysaccharide concentration was 100 mg/dl, about 20% of that seen after standard discontinuous flow leukapheresis. The average efficiency of granulocyte collection by this method is about 64%. The average yield of WBC from 2,600 ml of donors’ whole blood is 1.64 ± 0.54 x 10^10 (mean ± 1 SD) and the average granulocyte yield is 1.33 ± 0.51 x 10^10 (mean ± 1SD). The granulocytes were shown to migrate to the sites of infection in vivo. This approach is an improvement over standard discontinuous flow leukapheresis because of the high efficiency of granulocyte collection and because multiple procedures are possible without exposing donors to more HES than is currently given during a single procedure.