Efficacy of the De Novo-Derived Antimicrobial Peptide WLBU2 against Oral Bacteria

Novak, Karen F.; Diamond, William J.; Kirakodu, Sreenatha; Peyyala, Rebecca; Anderson, Kimberly W.; Montelaro, Ronald C.; Mietzner, Timothy A.

doi:10.1128/aac.00924-06

Cited by 12 publications

(12 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Although the number of P. gingivalis and P. inter- media cells in physiological saline spontaneously decreased, LFcin B at a relatively high concentration (0.4 mg/ml) further reduced the numbers of these bacteria. These results obtained with LFcin B seem to be in accord with those obtained in previous studies with other antimicrobial peptides, in which their activities against P. gingivalis and P. intermedia were weak or were not seen at all (8,29,32). P. gingivalis and P. intermedia colonize and grow as biofilms within subgingival plaque (14,27).…”

Section: Vol 53 2009supporting

confidence: 81%

Inhibitory Effects of Lactoferrin on Growth and Biofilm Formation ofPorphyromonas gingivalisandPrevotella intermedia

Wakabayashi

Yamauchi

Kobayashi

et al. 2009

Antimicrob Agents Chemother

114

View full text Add to dashboard Cite

Lactoferrin (LF) is an iron-binding antimicrobial protein present in saliva and gingival crevicular fluids, and it is possibly associated with host defense against oral pathogens, including periodontopathic bacteria. In the present study, we evaluated the in vitro effects of LF-related agents on the growth and biofilm formation of two periodontopathic bacteria, Porphyromonas gingivalis and Prevotella intermedia, which reside as biofilms in the subgingival plaque. The planktonic growth of P. gingivalis and P. intermedia was suppressed for up to 5 h by incubation with >130 g/ml of human LF (hLF), iron-free and iron-saturated bovine LF (apo-bLF and holo-bLF, respectively), and >6 g/ml of bLF-derived antimicrobial peptide lactoferricin B (LFcin B); but those effects were weak after 8 h. The biofilm formation of P. gingivalis and P. intermedia over 24 h was effectively inhibited by lower concentrations (>8 g/ml) of various iron-bound forms (the apo, native, and holo forms) of bLF and hLF but not LFcin B. A preformed biofilm of P. gingivalis and P. intermedia was also reduced by incubation with various iron-bound bLFs, hLF, and LFcin B for 5 h. In an examination of the effectiveness of native bLF when it was used in combination with four antibiotics, it was found that treatment with ciprofloxacin, clarithromycin, and minocycline in combination with native bLF for 24 h reduced the amount of a preformed biofilm of P. gingivalis compared with the level of reduction achieved with each agent alone. These results demonstrate the antibiofilm activity of LF with lower iron dependency against P. gingivalis and P. intermedia and the potential usefulness of LF for the prevention and treatment of periodontal diseases and as adjunct therapy for periodontal diseases.

show abstract

Section: Vol 53 2009supporting

confidence: 81%

Inhibitory Effects of Lactoferrin on Growth and Biofilm Formation ofPorphyromonas gingivalisandPrevotella intermedia

Wakabayashi

Yamauchi

Kobayashi

et al. 2009

Antimicrob Agents Chemother

114

View full text Add to dashboard Cite

show abstract

“…These are distinct from those in nature, with simpler but rationally engineered composition, obtained by varying the amino acid content and sequence and overall peptide length to achieve enhanced activity and very low cytotoxic properties [57]. For example, Mietzner and co-workers [58][59][60][61] have developed a series of de novo peptides based on structure-function properties observed in natural AMPs. They engineered the peptide composition to achieve enhanced potency, selectivity and stability.…”

Section: Antimicrobial Peptidesmentioning

confidence: 99%

Covalent immobilization of antimicrobial peptides (AMPs) onto biomaterial surfaces

et al. 2011

Self Cite

View full text Add to dashboard Cite

a b s t r a c tBacterial adhesion to biomaterials remains a major problem in the medical devices field. Antimicrobial peptides (AMPs) are well-known components of the innate immune system that can be applied to overcome biofilm-associated infections. Their relevance has been increasing as a practical alternative to conventional antibiotics, which are declining in effectiveness. The recent interest focused on these peptides can be explained by a group of special features, including a wide spectrum of activity, high efficacy at very low concentrations, target specificity, anti-endotoxin activity, synergistic action with classical antibiotics, and low propensity for developing resistance. Therefore, the development of an antimicrobial coating with such properties would be worthwhile. The immobilization of AMPs onto a biomaterial surface has further advantages as it also helps to circumvent AMPs' potential limitations, such as short half-life and cytotoxicity associated with higher concentrations of soluble peptides. The studies discussed in the current review report on the impact of covalent immobilization of AMPs onto surfaces through different chemical coupling strategies, length of spacers, and peptide orientation and concentration. The overall results suggest that immobilized AMPs may be effective in the prevention of biofilm formation by reduction of microorganism survival post-contact with the coated biomaterial. Minimal cytotoxicity and longterm stability profiles were obtained by optimizing immobilization parameters, indicating a promising potential for the use of immobilized AMPs in clinical applications. On the other hand, the effects of tethering on mechanisms of action of AMPs have not yet been fully elucidated. Therefore, further studies are recommended to explore the real potential of immobilized AMPs in health applications as antimicrobial coatings of medical devices.

show abstract

“…These sequences incorporate characteristics common to natural AMPs with changes designed to increase stability and function within a range of biological media. Deslouches and coworkers have designed de novo AMPs that display improved activity over a broad range of conditions including physiologic NaCl, in the presence of divalent cations, and in serum [161–163]. These peptides have also shown good selectivity for Pseudomonas aeruginosa over mammalian cells within a co-culture system [157] and excellent activity against both gram positive and gram negative multidrug resistant organisms [164].…”

Section: Antimicrobial Peptides (Amps) – Promising Therapeutics Fomentioning

confidence: 99%

Beyond conventional antibiotics — New directions for combination products to combat biofilm

Bayramov

Neff

2017

Advanced Drug Delivery Reviews

View full text Add to dashboard Cite

Medical device related infections are a significant and growing source of morbidity and mortality. Biofilm formation is a common feature of medical device infections that is not effectively prevented or treated by systemic antibiotics. Antimicrobial medical device combination products provide a pathway for local delivery of antimicrobial therapeutics with the ability to achieve high local concentrations while minimizing systemic side effects. In this review, we present considerations for the design of local antimicrobial delivery systems, which can be facilitated by modeling local pharmacokinetics in the context of the target device application. In addition to the need for local delivery, a critical barrier to progress in the field is the need to incorporate agents effective against biofilm. This article aims to review key properties of antimicrobial peptides that make them well suited to meet the demands of the next generation of antimicrobial medical devices, including broad spectrum activity, rapid and biocidal mechanisms of action, and efficacy against biofilm.

show abstract

Efficacy of the De Novo-Derived Antimicrobial Peptide WLBU2 against Oral Bacteria

Cited by 12 publications

References 21 publications

Inhibitory Effects of Lactoferrin on Growth and Biofilm Formation ofPorphyromonas gingivalisandPrevotella intermedia

Inhibitory Effects of Lactoferrin on Growth and Biofilm Formation ofPorphyromonas gingivalisandPrevotella intermedia

Covalent immobilization of antimicrobial peptides (AMPs) onto biomaterial surfaces

Beyond conventional antibiotics — New directions for combination products to combat biofilm

Contact Info

Product

Resources

About