Efficacy of systemic thrombolysis within 4.5 h from stroke symptom onset: a single-centre clinical and diffusion–perfusion 3T MRI study

Floris, Roberto; Cozzolino, Valeria; Meschini, Alessandro; Garaci, Francesco; Konda, Daniel; Marziali, Simone; Sallustio, Fabrizio; Legge, Silvia Di; Claroni, Giulia; Fanucci, E; Simonetti, Giovanni; Stanzione, Paolo

doi:10.1007/s11547-014-0394-z

Cited by 3 publications

(1 citation statement)

References 27 publications

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“…However, tPA therapy is beneficial only when given within a 3‐h window after the onset of a stroke and is associated with risk of hemorrhagic stroke when administered to older patients (Floris et al . ). Recent studies have indicated that tPA administration given beyond the traditional 3‐h window still has beneficial effects (Hacke et al .…”

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confidence: 97%

The mechanistic target of rapamycin (mTOR) pathway and S6 Kinase mediate diazoxide preconditioning in primary rat cortical neurons

Dutta

Rutkai

Katakam

et al. 2015

Journal of Neurochemistry

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We examined the role of the mechanistic target of rapamycin (mTOR) pathway in delayed diazoxide (DZ)-induced preconditioning of cultured rat primary cortical neurons. Neurons were treated for three days with 500 μM DZ or feeding medium and then exposed to three hours of continuous normoxia in DMEM with glucose or with three hours of oxygen-glucose deprivation (OGD) followed by normoxia and feeding medium. The OGD decreased viability by 50 percent, depolarized mitochondria and reduced mitochondrial respiration, whereas DZ treatment improved viability and mitochondrial respiration, and suppressed reactive oxygen species production, but did not restore mitochondrial membrane potential after OGD. Neuroprotection by DZ was associated with increased phosphorylation of protein kinase B (Akt), mTOR, and the major mTOR downstream substrate, S6 Kinase (S6K). The mTOR inhibitors rapamycin and Torin-1, as well as S6K-targeted siRNA abolished the protective effects of DZ. The effects of DZ on mitochondrial membrane potential and reactive oxygen species production were not affected by rapamycin. Preconditioning with DZ also changed mitochondrial and non-mitochondrial oxygen consumption rates. We conclude that in addition to reducing ROS production and mitochondrial membrane depolarization, DZ protects against OGD by activation of the Akt-mTOR-S6K pathway and by changes in mitochondrial respiration.

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confidence: 97%

The mechanistic target of rapamycin (mTOR) pathway and S6 Kinase mediate diazoxide preconditioning in primary rat cortical neurons

Dutta

Rutkai

Katakam

et al. 2015

Journal of Neurochemistry

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Numerical investigation of perfusion rates in the circle of Willis in different anatomical variations and ischemic stroke

2021

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The circle of Willis (CoW) is a set of arteries located in the basis of the brain. Prediction of perfusion rates and hemodynamics in the CoW is necessary to understand the relevant vascular diseases and to prescribe effective treatments. In this paper, the effect of ischemic stroke in the CoW is studied, taking into consideration the anatomical variations of the CoW. Moreover, an analysis on the effect of applied boundary conditions is carried out. To do so, a patient-specific model of the CoW is reconstructed from CT (computed tomography) images. Six different cases of boundary conditions are applied to complete and healthy CoW, and the flow rates are investigated. The proper pressure boundary conditions are then imposed to three other variations of the CoW, and the flow rates are compared. The results reveal that the overall inlet flow rate varies from 1.75% to 7.5% in three variations of healthy CoW. Moreover, the changes in flow rates of outlet and inlet branches are indicated in ischemic stroke by considering a spherical clot in the right middle cerebral artery (RMCA). In this case, the RMCA flow reduced by 88.4%, and the internal carotid artery flow decreased by 53.6%. These changes lead to increased flow rates of other inlets to support the brain; however, the overall inlet flow rate falls by 21.5%.

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Numerical simulation of a clot occlusion in the Circle of Willis

Darvish

Fatouraee

Nabaei

2019

2019 26th National and 4th International Iranian Conference on Biomedical Engineering (ICBME)

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Efficacy of systemic thrombolysis within 4.5 h from stroke symptom onset: a single-centre clinical and diffusion–perfusion 3T MRI study

Abstract: Treatment with IV rt-PA within 4.5 h of stroke onset preserves a significant amount of brain tissue from final infarction, and increases the possibility of early and late clinical improvement.

Cited by 3 publications

References 27 publications

The mechanistic target of rapamycin (mTOR) pathway and S6 Kinase mediate diazoxide preconditioning in primary rat cortical neurons

The mechanistic target of rapamycin (mTOR) pathway and S6 Kinase mediate diazoxide preconditioning in primary rat cortical neurons

Numerical investigation of perfusion rates in the circle of Willis in different anatomical variations and ischemic stroke

Numerical simulation of a clot occlusion in the Circle of Willis

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