Efficacy of<sup>99m</sup>Tc-Labeled Ceftizoxime in the Diagnosis of Subclinical Infections Associated with Titanium Implants in Rats

Moreira, TeixeiraLuiz Eduardo; Gontijo, SoaresGuilherme; Cembranelli, TeixeiraHenrique; Magalhães, TakenakaIsabella Kuniko Tavares; Fernandes, DinizSimone Odília; Percope, de AndradeMarco Antônio; Nascimento, CardosoValbert; Duval, de AraújoIvana

doi:10.1089/sur.2014.186

Cited by 13 publications

(14 citation statements)

References 22 publications

(26 reference statements)

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“…It was found that our radiochemical complex in aqueous phase was detected at R.f = 1 as 98.8% pure while in previous published study it was 92%. As compared to previous published study, our synthesized radio complex was hydrophilic, showed mild retention of activity in liver and lungs and excreted through kidney. In vivo distribution of synthesized complex was compared with the unlabeled ceftizoxime published in research article and it was found that it followed the same path of distribution as unlabeled ceftizoxime…”

Section: Discussionmentioning

confidence: 51%

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^99mTc‐Ceftizoxime: Synthesis, characterization and its use in diagnosis of diabetic foot osteomyelitis

Ahmed

Fatima²,

Saeed³

et al. 2018

J Med Imag Rad Onc

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Introduction: The diagnosis of diabetic foot osteomyelitis is crucial and challenging for the proper management of diabetic foot. 99m Tc labelled Ceftizoxime was used as a non-invasive diagnostic agent for diabetic foot osteomyelitis. Methods: Ceftizoxime [CFT], a third generation cephalosporin, was used in a simple and direct method for the synthesis of 99m TcO 4 À labelled infection imaging agent with stannous chloride as reducing agent. Its radiochemical purity was checked by thin Layer chromatography. Partition co-efficient was measured with phosphate buffer and chloroform. The radiochemical complex was injected to control and infected animal model for 3 hours in-vivo localization studied with the help of dual head gamma camera. The labelled complexes were injected to 5 patients of known type II diabetes mellitus suspected of diabetic foot osteomyelitis. All patients underwent dynamic and static 99m Tc-MDP and 99m Tc-CFT scans.Results: The synthesized radio labelled complex was 98.8% pure, with hydrophilic character. When injected to animal model, at 120 minutes, 49.3% was localized in foci of infection with 3.35% in liver and excretion through kidney. Human studies were interpreted as true or false positive and true or false negative based on bone histopathology/culture and clinical follow-up. We found that of 5 patients, 2 were true positive, 2 as true negative with no false positive or negative and 01 patient had soft tissue infection.Conclusion: This study showed that 99m Tc-CFT labelled complex could be used for detection of diabetic foot osteomyelitis; however, further confirmation of results with a larger patient population would be optimal.Key words: 99m Tc labelled antibiotic; bone infection imaging agent; non-invasive imaging agent; 99m Tc-Ceftizoxime as bone osteomyelitis imaging agent.

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Section: Discussionmentioning

confidence: 51%

“…Radio labelled antibiotics can serve effectively in this stance . 99m Tc labelled antibiotics like ciprofloxacin (Infecton) has been used in a number of human studies like bone infection with variable results and in diabetic foot infection and some animal studies of 99m Tc ceftizoxime by other group, except 99m Tc‐UBI (29‐41) peptide…”

Section: Introductionmentioning

confidence: 99%

^99mTc‐Ceftizoxime: Synthesis, characterization and its use in diagnosis of diabetic foot osteomyelitis

Ahmed

Fatima²,

Saeed³

et al. 2018

J Med Imag Rad Onc

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“…In our study, we used a third-generation cephalosporin as the radiopharmaceutical that is active against gram-positive, gram-negative, and anaerobic bacteria. Moreover, its application as a radiopharmaceutical has shown to be effective for soft tissue infections, osteomyelitis, and prosthetic infections 10,11,12,14,15 .…”

Section: ■ Discussionmentioning

confidence: 99%

“…2018;33(1): [14][15][16][17][18][19][20][21] polyvinylpyrrolidone-iodine (Riodine ® ) in an antiseptic solution, followed by an alcoholbased solution, and protection with sterile fields. A longitudinal incision was formed at the thigh, dissecting the layers to expose the posterior diaphyseal region of the femur.…”

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confidence: 99%

Influence of biomaterials on scintigraphic diagnosis of periprosthetic infections. Ceftizoxime-99m technetium model

et al. 2018

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“…Also Teixeira et al [86] used 99m Tc-ceftizoxime for the diagnosis of suspected infections in titanium implants in rat model. Control rats received a sterile implant, while experimental group received an implant infected with 10 9 CFU of S. aureus .…”

Section: Cephalosporinsmentioning

confidence: 99%

Imaging bacteria with radiolabelled quinolones, cephalosporins and siderophores for imaging infection: a systematic review

Auletta

Galli

Lauri

et al. 2016

Clin Transl Imaging

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Bacterial infections are still one of the main causes of patient morbidity and mortality worldwide. Nowadays, many imaging techniques, like computed tomography or magnetic resonance imaging, are used to identify inflammatory processes, but, although they recognize anatomical modifications, they cannot easily distinguish bacterial infective foci from non bacterial infections. In nuclear medicine, many efforts have been made to develop specific radiopharmaceuticals to discriminate infection from sterile inflammation. Several compounds (antimicrobial peptides, leukocytes, cytokines, antibiotics…) have been radiolabelled and tested in vitro and in vivo, but none proved to be highly specific for bacteria. Indeed factors, including the number and strain of bacteria, the infection site, and the host condition may affect the specificity of tested radiopharmaceuticals. Ciprofloxacin has been proposed and intensively studied because of its easy radiolabelling method, broad spectrum, and low cost, but at the same time it presents some problems such as low stability or the risk of antibiotic resistance. Therefore, in the present review studies with ciprofloxacin and other radiolabelled antibiotics as possible substitutes of ciprofloxacin are reported. Among them we can distinguish different classes, such as cephalosporins, fluoroquinolones, inhibitors of nucleic acid synthesis, inhibitors of bacterial cell wall synthesis and inhibitors of protein synthesis; then also others, like siderophores or maltodextrin-based probes, have been discussed as bacterial infection imaging agents. A systematic analysis was performed to report the main characteristics and differences of each antibiotic to provide an overview about the state of the art of imaging infection with radiolabelled antibiotics.

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Efficacy of^99mTc-Labeled Ceftizoxime in the Diagnosis of Subclinical Infections Associated with Titanium Implants in Rats

Cited by 13 publications

References 22 publications

^99mTc‐Ceftizoxime: Synthesis, characterization and its use in diagnosis of diabetic foot osteomyelitis

^99mTc‐Ceftizoxime: Synthesis, characterization and its use in diagnosis of diabetic foot osteomyelitis

Influence of biomaterials on scintigraphic diagnosis of periprosthetic infections. Ceftizoxime-99m technetium model

Imaging bacteria with radiolabelled quinolones, cephalosporins and siderophores for imaging infection: a systematic review

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Efficacy of99mTc-Labeled Ceftizoxime in the Diagnosis of Subclinical Infections Associated with Titanium Implants in Rats

Cited by 13 publications

References 22 publications

99mTc‐Ceftizoxime: Synthesis, characterization and its use in diagnosis of diabetic foot osteomyelitis

99mTc‐Ceftizoxime: Synthesis, characterization and its use in diagnosis of diabetic foot osteomyelitis

Influence of biomaterials on scintigraphic diagnosis of periprosthetic infections. Ceftizoxime-99m technetium model

Imaging bacteria with radiolabelled quinolones, cephalosporins and siderophores for imaging infection: a systematic review

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Efficacy of^99mTc-Labeled Ceftizoxime in the Diagnosis of Subclinical Infections Associated with Titanium Implants in Rats

^99mTc‐Ceftizoxime: Synthesis, characterization and its use in diagnosis of diabetic foot osteomyelitis

^99mTc‐Ceftizoxime: Synthesis, characterization and its use in diagnosis of diabetic foot osteomyelitis