Efficacy of spent blewit mushroom compost andBacillus aryabhattaicombination on control of Pythium damping-off in cucumber

Abstract

SUMMARYPythium damping-off caused by Pythium aphanidermatum usually occurs in commercial nurseries of cucumber seedlings. The purpose of the present study was to develop a container medium for effectively suppressing Pythium damping-off of cucumber seedlings. Seven agricultural materials were tested for their suitability as substrates for the growth of cucumber seedlings. Bas Van Burren No. 4 peat moss (BVB) was able to promote the growth of cucumber seedlings, but it was ineffective against Pythium damping-off. Spent blewit mushroom compost (SBMC) was an available substrate that could inhibit P. aphanidermatum, but it also inhibited the growth of cucumber seedlings slightly. A cultural medium, spent blewit peat compost (SBPC), containing 50% (v/v) SBMC, 50% (v/v) BVB and 0·3% (w/v) lime was consequently formulated. The SBPC was able to promote the growth of cucumber seedlings and reduce the incidence of Pythium damping-off. A total of 20 micro-organisms were isolated from SBMC compost and screened in steamed SBPC medium for their ability to inhibit the growth of P. aphanidermatum. Among these, Bacillus aryabhattai isolate number CB13 was able to suppress P. aphanidermatum significantly in the steamed SBPC medium. Thus, bio-formulation of SBPC medium with the beneficial microbe B. aryabhattai CB13 was carried out. The incidence of Pythium damping-off was reduced from 58% in steamed SBPC medium to 4% in the bio-formulated container medium. The novel bio-formulation has high potential for controlling Pythium damping-off in commercial nurseries.

Streptomyces Application Triggers Reassembly and Optimization of the Rhizosphere Microbiome of Cucumber

“…On the other hand, Firmicutes also increased following Streptomyces treatment, which is consistent with a previous study that the enhanced Firmicutes was also observed when the same Streptomyces treatment was applied on tomato [15]. The phylum Firmicutes comprises the class Bacillus, and the function of growth promotion and disease resistance has been reported in the genus Bacillus aryabhattai [37,38], Bacillus subtilis [39,40], and Bacillus amyloliquefaciens [41,42]. The enhancement of this phylum in the present study indicates that the stress resistance of the microbiota in the rhizosphere was enhanced by Streptomyces.…”

Suppressive efficacy of volatile compounds produced byBacillus mycoideson damping-off pathogens of cabbage seedlings

“…lycopersici and powdery mildew (Ding and Huang, 2017). A combination of spent blewit mushroom compost and B. aryabhattai has recently been shown to control Pythium damping-off in cucumber (Chen et al ., 2015). A B. mycoides strain isolated from the rice rhizosphere has recently been shown to produce biosurfactants, suppress the formation of zoospores by P. aphanidermatum and reduce damping-off by 35% in cucumber (Peng et al ., 2017).…”

Growth, Nutrient Uptake, and Foliar Gas Exchange in Pepper Cultured with Un-composted Fresh Spent Mushroom Residue

“…Therefore, SMC has been aroused as an alternative to the commercial peat substrate (Kandemir et al, 2009;Ünal, 2015;Zhang et al, 2012Zhang et al, , 2013. The use of SMC in the container substrate can efficiently inhibit the pathogen damage on horticultural crops (Segarra et al, 2007;Chen et al, 2015), promote nutrient uptake (Paredes et al, 2016;Sönmez et al, 2016), and promote crop growth (Ünal, 2015). However, the high pH value and electrical conductance (EC) prevent SMS to be used as the main component in the substrate (Szmidt and Chong, 1995;Gonani et al, 2011).…”