These experiments were conducted to control the root-knot nematodes, Meloidogyne incognita under both greenhouse and field conditions in superior seedless vineyards and its reflection on yield. The field experiment was carried out in a private vineyard (El Wady El Faregh) in Alexandria-Cairo road (kilo 78). Seven treatments were used as follows: two bioagents (Bacillus subtilis and Trichoderma harzianum), two blue green strains algal species (Cyanobacteria) (Spirulina platensis and Oscillatoria sp.) and two plant aqueous extracts (Ambrosia maritime and Eucalyptus globules) at three concentrations, and in comparison with oxamyl (24% EC) were used to control Meloidogyne incognita on superior seedless vineyards on grapes under both greenhouse and field conditions. Bioagents, algal species and plant extracts were added to the soil under vines one time weekly for three weeks after bud burst under both greenhouse and field conditions. The most effective treatments in controlling root-knot nematodes; Meloidogyne incognita were both Spirulina platensis and Oscillatoria sp whereas the least effective was suspension of Eucalyptus globules under both greenhouse and field conditions. Both Spirulina platensis and Oscillatoria sp acheived the highest reduction effect on number of 2 nd stage juveniles in soil, numbers of galls, developmental stages, females, egg masses, egg numbers/egg mass in roots and final population Meloidogyne incognita on grape plants, whereas the suspension of Eucalyptus globules was the least effective treatment under both greenhouse and field conditions. In addition all treatments increased fresh weight of the whole plant over control under greenhouse conditions. Results showed that all the treatments had a significant effect in reducing the total population and build up of root-knot nematodes, Meloidogyne incognita in both soil and roots especially after three months from the time of application. In addition, all treatments had increased the yield of superior seedless grapevines under field conditions.