Efficacy of sodium butyrate adjunct therapy in shigellosis: a randomized, double-blind, placebo-controlled clinical trial

Raqib, Rubhana; Sarker, Protim; Mily, Akhirunnesa; Alam, Nur; Arifuzzaman, Abu Saleh Mohammed; Rekha, Rokeya Sultana; Andersson, Jan; Guðmundsson, Guðmundur H.; Cravioto, Alejandro; Agerberth, Birgitta

doi:10.1186/1471-2334-12-111

Cited by 77 publications

(56 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In this study, we have confirmed that butyrate is a strong inducer of multiple β-defensin and cathelicidin genes in different porcine cell types. It is known that LL-37 mRNA and protein expressions are concurrently augmented in humans and rabbits following butyrate administration [25], [38], and that butyrate-induced HDP mRNA expression is associated with an enhanced antibacterial activity of monocytes and bacterial clearance in chickens [26]. It is still important to confirm the up-regulation of porcine HDPs at the protein levels, particularly in vivo, and the subsequent effect on disease resistance and pathogen reduction in pigs in the future.…”

Section: Discussionmentioning

confidence: 99%

Induction of Porcine Host Defense Peptide Gene Expression by Short-Chain Fatty Acids and Their Analogs

et al. 2013

View full text Add to dashboard Cite

Dietary modulation of the synthesis of endogenous host defense peptides (HDPs) represents a novel antimicrobial approach for disease control and prevention, particularly against antibiotic-resistant infections. However, HDP regulation by dietary compounds such as butyrate is species-dependent. To examine whether butyrate could induce HDP expression in pigs, we evaluated the expressions of a panel of porcine HDPs in IPEC-J2 intestinal epithelial cells, 3D4/31 macrophages, and primary monocytes in response to sodium butyrate treatment by real-time PCR. We revealed that butyrate is a potent inducer of multiple, but not all, HDP genes. Porcine β-defensin 2 (pBD2), pBD3, epididymis protein 2 splicing variant C (pEP2C), and protegrins were induced markedly in response to butyrate, whereas pBD1 expression remained largely unaltered in any cell type. Additionally, a comparison of the HDP-inducing efficacy among saturated free fatty acids of different aliphatic chain lengths revealed that fatty acids containing 3–8 carbons showed an obvious induction of HDP expression in IPEC-J2 cells, with butyrate being the most potent and long-chain fatty acids having only a marginal effect. We further investigated a panel of butyrate analogs for their efficacy in HDP induction, and found glyceryl tributyrate, benzyl butyrate, and 4-phenylbutyrate to be comparable with butyrate. Identification of butyrate and several analogs with a strong capacity to induce HDP gene expression in pigs provides attractive candidates for further evaluation of their potential as novel alternatives to antibiotics in augmenting innate immunity and disease resistance of pigs.

show abstract

Section: Discussionmentioning

confidence: 99%

Induction of Porcine Host Defense Peptide Gene Expression by Short-Chain Fatty Acids and Their Analogs

et al. 2013

View full text Add to dashboard Cite

show abstract

“…By providing nutrition to saprophytic intestinal bacteria it helps maintain the proper composition of intestinal bacterial flora, thus preventing absorption of toxins and development of harmful bacteria [10–24]. The presence of bacteria-producing short-chain fatty acids has been found to inhibit Escherichia coli, Campylobacter and Salmonella species both in the mechanism of competitive colonisation and antibacterial molecule secretion with immunological stimulation.…”

Section: Discussionmentioning

confidence: 99%

Management of traveller’s diarrhoea with a combination of sodium butyrate, organic acids, and A-300 silicon dioxide

Krokowicz

Mackiewicz

Wejman-Matela

et al. 2014

View full text Add to dashboard Cite

IntroductionTraveller's diarrhoea (TD), defined by UNICEF/WHO as three or more unformed stools with or without other symptoms, imposes a considerable burden on travellers from developed countries. Various efforts have focused on decreasing the prevalence and severity of this condition.AimTo assess the efficacy of a combination of sodium butyrate, organic acids, and A-300 silicon dioxide in treatment providing symptomatic relief of TD.Material and methodsThe study was conducted in accordance with a protocol presented to the Bioethical committee of Poznan University of Medical Sciences. A total of 278 patients travelling to countries with higher risk of diarrhoea for at least 10 days were divided into a study arm being administered, in case of TD, a combination of sodium butyrate, organic acids, and A-300 silicon dioxide (n = 139) and a placebo arm (n = 139) with placebo administration.ResultsForty-seven patients completed the study (22 in the study arm and 25 in the placebo arm). The diarrhoea occurrence after initiation of treatment at first symptoms was significantly lower in the study arm as compared to the placebo arm (9% vs. 36%, p = 0.041). Also, subjects from the study arm more frequently reported that the regimen administered had been efficient for their symptoms in comparison to the placebo arm (72.7% vs. 32%, p = 0.008). No adverse effects of the administered medication were noted during the study.ConclusionsSodium butyrate, organic acids, and A-300 silicon dioxide can be successful in decreasing symptoms of TD. Because of its efficacy and lack of observed side effects it has a strong potential in the treatment of patients with TD.

show abstract

“…Accumulating evidence has shown that butyrate could attenuate bacterial translocation across epithelia under metabolic stress [65], and enhance the gut barrier via augmenting tight junction assembly [66]. In addition, a randomized, double-blind clinical trial has revealed the effects of butyrate as an adjunct therapy in combination with antibiotics on the treatment of shigellosis patients [67]. …”

Section: Reviewmentioning

confidence: 99%

Role of intestinal microbiota and metabolites on gut homeostasis and human diseases

2017

View full text Add to dashboard Cite

BackgroundA vast diversity of microbes colonizes in the human gastrointestinal tract, referred to intestinal microbiota. Microbiota and products thereof are indispensable for shaping the development and function of host innate immune system, thereby exerting multifaceted impacts in gut health.MethodsThis paper reviews the effects on immunity of gut microbe-derived nucleic acids, and gut microbial metabolites, as well as the involvement of commensals in the gut homeostasis. We focus on the recent findings with an intention to illuminate the mechanisms by which the microbiota and products thereof are interacting with host immunity, as well as to scrutinize imbalanced gut microbiota (dysbiosis) which lead to autoimmune disorders including inflammatory bowel disease (IBD), Type 1 diabetes (T1D) and systemic immune syndromes such as rheumatoid arthritis (RA).ResultsIn addition to their well-recognized benefits in the gut such as occupation of ecological niches and competition with pathogens, commensal bacteria have been shown to strengthen the gut barrier and to exert immunomodulatory actions within the gut and beyond. It has been realized that impaired intestinal microbiota not only contribute to gut diseases but also are inextricably linked to metabolic disorders and even brain dysfunction.ConclusionsA better understanding of the mutual interactions of the microbiota and host immune system, would shed light on our endeavors of disease prevention and broaden the path to our discovery of immune intervention targets for disease treatment.

show abstract

Efficacy of sodium butyrate adjunct therapy in shigellosis: a randomized, double-blind, placebo-controlled clinical trial

Cited by 77 publications

References 31 publications

Induction of Porcine Host Defense Peptide Gene Expression by Short-Chain Fatty Acids and Their Analogs

Induction of Porcine Host Defense Peptide Gene Expression by Short-Chain Fatty Acids and Their Analogs

Management of traveller’s diarrhoea with a combination of sodium butyrate, organic acids, and A-300 silicon dioxide

Role of intestinal microbiota and metabolites on gut homeostasis and human diseases

Contact Info

Product

Resources

About