Efficacy of single high-molecular-weight versus triple low-molecular-weight hyaluronic acid intra-articular injection among knee osteoarthritis patients

Bahrami, Mohammad Hasan; Raeissadat, Seyed Ahmad; Cheraghi, Mohsen; Rahimi‐Dehgolan, Shahram; Ebrahimpour, Adel

doi:10.1186/s12891-020-03577-8

Cited by 19 publications

(13 citation statements)

References 43 publications

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“…It is well established that low molecular weight proteins have high expression and better folding compared to larger ones, so we aimed to remove PDZ domains from trypsin‐like domains using protein engineering. PDZ domains normally have a regulatory function in the complete enzyme construct 7,16 . For truncating these domains, a stop codon was introduced through site‐directed mutagenesis at 249 positions in the protein sequence (Figure 1) and further confirmed by the Sanger sequencing.…”

Section: Resultsmentioning

confidence: 99%

“…PDZ domains normally have a regulatory function in the complete enzyme construct. 7,16 For truncating these domains, a stop codon was introduced through sitedirected mutagenesis at 249 positions in the protein sequence (Figure 1) and further confirmed by the Sanger sequencing. The mutated plasmid was transformed to BL21(DE3) for over-expression.…”

Section: Expression Purification and Characterization Of Trypsin-like...mentioning

confidence: 99%

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Expression and immobilization of trypsin‐like domain of serine protease from Pseudomonas aeruginosa for improved stability and catalytic activity

et al. 2022

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Protein engineering and enzyme immobilization strategies have produced numerous biocatalysts for modern industrial applications. In this study, we have also used these two strategies for improving the operational stability and catalytic efficiency of serine protease from Pseudomonas aeruginosa. The enzyme serine protease was truncated to separate its trypsin‐like domain from the PDZ1 and PDZ2 domains. The truncated trypsin‐like domain was expressed in Escherichia coli BL21, and its catalytic activity and thermostability were estimated. Later this trypsin‐like domain was immobilized with 2% Na‐alginate. The immobilized domain showed 10°C increase in optimum temperature compared to its free counterpart. Kinetic studies showed two‐folds increased Vmax of the immobilized domain. Likewise, the Km value of this domain was 11.5 folds lower compared to the free trypsin‐like domain. The catalytic efficiency (Kcat/Km) of the immobilized enzyme also elevated to 311 folds. Additionally, the immobilized trypsin‐like domain remained active in the presence of surfactants (Triton‐X 100, SDS, and Tween‐40) and metal ions (Mg2+, Ca2+, Na+, and Zn2+). It also efficiently removes gelatin layer from X‐ray film and hair from sheepskin. Thus, the immobilized trypsin‐like domain of serine protease, with increased thermostability and catalytic efficiency, is operationally more stable than the soluble truncated trypsin‐like domain.

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Section: Resultsmentioning

confidence: 99%

Section: Expression Purification and Characterization Of Trypsin-like...mentioning

confidence: 99%

Expression and immobilization of trypsin‐like domain of serine protease from Pseudomonas aeruginosa for improved stability and catalytic activity

et al. 2022

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“…209 High MW would provide longer lasting protective effects in OA-affected joints. 210 The capacity of HA to penetrate the cartilage ECM and directly modulate chondrocyte activity remains a matter of debate. A drawback of HA therapy is the preferred i.a.…”

Section: Restorage Of Joint Function By Viscosupplementation and Restmentioning

confidence: 99%

Experimental Therapeutics for the Treatment of Osteoarthritis

Schulze‐Tanzil

2021

JEP

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Osteoarthritis (OA) therapy remains a large challenge since no causative treatment options are so far available. Despite some main pathways contributing to OA are identified its pathogenesis is still rudimentary understood. A plethora of therapeutically promising agents are currently tested in experimental OA research to find an opportunity to reverse OA-associated joint damage and prevent its progression. Hence, this review aims to summarize novelly emerging experimental approaches for OA. Due to the diversity of strategies shown only main aspects could be summarized here including herbal medicines, nanoparticular compounds, growth factors, hormones, antibody-, cell-and extracellular vesicle (EV)-based approaches, optimized tools for joint viscosupplementation, genetic regulators such as si-or miRNAs and promising combinations. An abundant multitude of compounds obtained from plants, environmental, autologous or synthetic sources have been identified with anabolic, anti-inflammatory,-catabolic and anti-apoptotic properties. Some ubiquitous signaling pathways such as wingless and Integration site-1 (Wnt), Sirtuin, Tolllike receptor (TLR), mammalian target of rapamycin (mTOR), Nuclear Factor (NF)-κB and complement are involved in OA and addressed by them. Hyaluronan (HA) provided benefit in OA since many decades, and novel HA formulations have been developed now with higher HA content and long-term stability achieved by cross-linking suitable to be combined with other agents such as components from herbals or chemokines to attract regenerative cells. pH-or inflammation-sensitive nanoparticular compounds could serve as versatile slowrelease systems of active compounds, for example, miRNAs. Some light has been brought into the intimate regulatory network of small RNAs in the pathogenesis of OA which might be a novel avenue for OA therapy in future. Attraction of autologous regenerative cells by chemokines and exosome-based treatment strategies could also innovate OA therapy.

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“…Many papers have reported that high MW CL HA is more effective than low MW HA. It has been reported that one administration of high MW HA has a similar effect to three administrations of low MW [85,86]. However, due to the specificity of crosslinking, pathological development of granulomatous inflammation, a pseudoseptic response, is also known [75].…”

Section: Cross-linked Hyaluronic Acidmentioning

confidence: 99%

Viscosupplementation in the Therapy for Osteoarthritic Knee

Park

Rho

et al. 2021

Applied Sciences

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Osteoarthritis (OA) is a degenerative disease that is spreading worldwide due to an aging population. This is not simply a disease caused by worn out joints, but a complex disease accompanied by various mechanisms such as inflammatory reactions. Among various joints, knee joints show degenerative changes earlier than other joints because they carry most of the weight load, causing social-economic problems. In the case of OA of the knee that does not respond to relatively simple conservative treatments such as physical therapy or medication, intra-articular injection is preferred. However, intra-articular injection drugs have limited effectiveness and uncertainties. There are several intra-articular viscous supplement drugs such as hyaluronic acid. Tissue regeneration active materials such as polydeoxyribonucleotide and polynucleotide are also newly used. The objective of this paper was to compare effects of intra-articular supplementation drugs used for degenerative arthritis of the knee.

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Efficacy of single high-molecular-weight versus triple low-molecular-weight hyaluronic acid intra-articular injection among knee osteoarthritis patients

Cited by 19 publications

References 43 publications

Expression and immobilization of trypsin‐like domain of serine protease from Pseudomonas aeruginosa for improved stability and catalytic activity

Expression and immobilization of trypsin‐like domain of serine protease from Pseudomonas aeruginosa for improved stability and catalytic activity

Experimental Therapeutics for the Treatment of Osteoarthritis

Viscosupplementation in the Therapy for Osteoarthritic Knee

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