Efficacy of short-synthetic antifungal peptides on pathogenic Aspergillus flavus

Devi, Sri; Raj, Navya; Sashidhar, R.B.

doi:10.1016/j.pestbp.2021.104810

Cited by 11 publications

(3 citation statements)

References 57 publications

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“…Generally, cells can alleviate ROS damage to cells through the action of enzymes (superoxide dismutase, catalase). Some small molecules, such as glutathione, also play critical cellular antioxidants (75). In our research, the gene (TR16620_c0_g1) relative to peroxiredoxin activity was upregulated by 3.06-fold.…”

Section: Genes Related To Stress Responsementioning

confidence: 50%

Mining RNA-Seq Data to Depict How Penicillium digitatum Shapes Its Transcriptome in Response to Nanoemulsion

et al. 2021

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Penicillium digitatum is the most severe pathogen that infects citrus fruits during storage. It can cause fruit rot and bring significant economic losses. The continuous use of fungicides has resulted in the emergence of drug-resistant strains. Consequently, there is a need to develop naturally and efficiently antifungal fungicides. Natural antimicrobial agents such as clove oil, cinnamon oil, and thyme oil can be extracted from different plant parts. They exhibited broad-spectrum antimicrobial properties and have great potential in the food industry. Here, we exploit a novel cinnamaldehyde (CA), eugenol (EUG), or carvacrol (CAR) combination antifungal therapy and formulate it into nanoemulsion form to overcome lower solubility and instability of essential oil. In this study, the antifungal activity evaluation and transcriptional profile of Penicillium digitatum exposed to compound nanoemulsion were evaluated. Results showed that compound nanoemulsion had a striking inhibitory effect on P. digitatum in a dose-dependent manner. According to RNA-seq analysis, there were 2,169 differentially expressed genes (DEGs) between control and nanoemulsion-treated samples, including 1,028 downregulated and 1,141 upregulated genes. Gene Ontology (GO) analysis indicated that the DEGs were mainly involved in intracellular organelle parts of cell component: cellular respiration, proton transmembrane transport of biological process, and guanyl nucleotide-binding molecular function. KEGG analysis revealed that metabolic pathway, biosynthesis of secondary metabolites, and glyoxylate and dicarboxylate metabolism were the most highly enriched pathways for these DEGs. Taken together, we can conclude the promising antifungal activity of nanoemulsion with multiple action sites against P. digitatum. These outcomes would deepen our knowledge of the inhibitory mechanism from molecular aspects and exploit naturally, efficiently, and harmlessly antifungal agents in the citrus postharvest industry.

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Section: Genes Related To Stress Responsementioning

confidence: 50%

Mining RNA-Seq Data to Depict How Penicillium digitatum Shapes Its Transcriptome in Response to Nanoemulsion

et al. 2021

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“…Quantitative real time polymerase chain reaction (RT-qPCR) analysis of the aflatoxin gene cluster showed that the aflR gene, and the downstream genes were significantly downregulated. The involvement of oxidative stress in the effect mediated by these peptides was also recently analyzed [218]. Results revealed that high peptide concentrations induced oxidative stress in A. flavus, while a complete inhibition of AF production was not observed, even though a four to five-fold reduction occurred.…”

Section: Effects Of Distinct Amps On Mycotoxin-productionmentioning

confidence: 99%

Antifungal Peptides and Proteins to Control Toxigenic Fungi and Mycotoxin Biosynthesis

Martínez-Culebras

Gandía

Garrigues

et al. 2021

IJMS

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The global challenge to prevent fungal spoilage and mycotoxin contamination on food and feed requires the development of new antifungal strategies. Antimicrobial peptides and proteins (AMPs) with antifungal activity are gaining much interest as natural antifungal compounds due to their properties such as structure diversity and function, antifungal spectrum, mechanism of action, high stability and the availability of biotechnological production methods. Given their multistep mode of action, the development of fungal resistance to AMPs is presumed to be slow or delayed compared to conventional fungicides. Interestingly, AMPs also accomplish important biological functions other than antifungal activity, including anti-mycotoxin biosynthesis activity, which opens novel aspects for their future use in agriculture and food industry to fight mycotoxin contamination. AMPs can reach intracellular targets and exert their activity by mechanisms other than membrane permeabilization. The mechanisms through which AMPs affect mycotoxin production are varied and complex, ranging from oxidative stress to specific inhibition of enzymatic components of mycotoxin biosynthetic pathways. This review presents natural and synthetic antifungal AMPs from different origins which are effective against mycotoxin-producing fungi, and aims at summarizing current knowledge concerning their additional effects on mycotoxin biosynthesis. Antifungal AMPs properties and mechanisms of action are also discussed.

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“…Peptides have numerous advantages, including easy availability, purification and storage, high potential and specificity, good biodegradability and biocompatible properties, and low toxicity (Qi et al ., 2018). These favorable characteristics have allowed them to be used for different purposes, including as antifungal, antimicrobial, anticancer, antioxidant, mineral binding, antithrombotic, antihypertensive, and immunomodulatory agents, and even they are used in cosmetics to provide benefits to the skin through the topical application (Xiao et al ., 2015; Schagen, 2017; Chen & Lu, 2020; Manju Devi et al ., 2021). Peptides have also attracted the attention of research groups for the development of novel gene therapies; the mechanism of action of each treatment varies depending on the function, the purpose, and the nature of the peptide (Lau & Dunn, 2018).…”

Section: Introductionmentioning

confidence: 99%

Bioactive peptides from nuts: A review

Acevedo‐Juárez

Guajardo‐Flores

Heredia‐Olea

et al. 2022

Int J of Food Sci Tech

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Summary Oxidative stress occurs because of an imbalance in the production of reactive oxygen species. Nuts are rich in bioactive compounds, including phenolic compounds, tannins, and phytosterols. Although nuts are widely consumed because of their beneficial effects on nutrition and health, there is limited information about bioactive peptides from nuts. Pine nut and walnut‐derived peptides are the most studied because these nuts contain a higher amount of protein. Different biological activities have been demonstrated for nut peptides; many of them exhibit antioxidant, anticancer, antihypertensive, antidiabetic, immunomodulatory, antiseizure, or neuroprotective activity. Recent studies have focused on increasing the bioactivity of identified bioactive peptides by applying new technologies and chemosynthetic strategies. Research tendency points to the generation of peptides with specific sequences for application in specific diseases. Nut bioactive peptides can become key functional ingredients for food, pharmaceuticals, or cosmetics.

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Efficacy of short-synthetic antifungal peptides on pathogenic Aspergillus flavus

Cited by 11 publications

References 57 publications

Mining RNA-Seq Data to Depict How Penicillium digitatum Shapes Its Transcriptome in Response to Nanoemulsion

Mining RNA-Seq Data to Depict How Penicillium digitatum Shapes Its Transcriptome in Response to Nanoemulsion

Antifungal Peptides and Proteins to Control Toxigenic Fungi and Mycotoxin Biosynthesis

Bioactive peptides from nuts: A review

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