Efficacy of serum samples stored on filter paper for the detection of antibody to Leptospira spp. by microagglutination test (MAT)

Blanco, Roberta Morozetti; Romero, Eliete Caló

doi:10.1016/j.jim.2012.08.013

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“…To overcome the problem of sample transportation to reference laboratories, leptospirosis serology by microagglutination testing (MAT) of sera spotted on FP has been evaluated (17). Leptospirosis MAT of sera spotted on FP had some limitations, because a decrease of sensitivity for storage up to 7 days has been reported.…”

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confidence: 99%

Sensitivity of Real-Time PCR Performed on Dried Sera Spotted on Filter Paper for Diagnosis of Leptospirosis

Nhan¹,

Teissier²,

Roche³

et al. 2014

J Clin Microbiol

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In remote countries, leptospirosis confirmation is difficult because it requires the shipment of frozen samples to reference laboratories. The sensitivity of leptospirosis real-time PCR performed on filter paper-dried serum samples stored at ambient temperature was evaluated at 2 ؋ 10 2 equivalent leptospires/ml (eqLeptospires/ml). This easy alternative procedure can be used to enhance the surveillance of leptospirosis.

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confidence: 99%