Efficacy of scallop shell powders and slaked lime for inactivating avian influenza virus under harsh conditions

Thammakarn, Chanathip; Tsujimura, Misato; Satoh, Katsuya; Hasegawa, Tomomi; Tamura, Miho; Kawamura, Akinobu; Ishida, Yuichi; Suguro, Atsushi; Hakim, Hakimullah; Ruenphet, Sakchai; Takehara, Kazuhiko

doi:10.1007/s00705-015-2517-9

Cited by 27 publications

(26 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In harsh conditions, it is reported that slaked lime maintained its virucidal efficacy (RF>3) under sunlight for 3 weeks and that it showed high efficacy (RF>3) under wet and dry conditions for 3 times resuspension when the samples were evaluated using LPAIV H7N1 for 3 min [ 21 ]. In addition, it is suggested that after rain, slaked lime became hardened and it was difficult to take samples from the surface of the lime [ 22 ].…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Calcinated egg shell as a candidate of biosecurity enhancement material

Ota

Toyofuku

Thammakarn

et al. 2016

The Journal of Veterinary Medical Science

Self Cite

View full text Add to dashboard Cite

Calcinated egg shell (Egg-CaO), of which the main component is calcium oxide, was evaluated in the forms of powder and aqueous solutions for their efficacies as disinfectants against avian influenza virus (AIV), Newcastle disease virus (NDV), infectious bursal disease virus (IBDV), Salmonella Infantis and Escherichia coli. Egg-CaO powder inactivated these viruses within 3 min in the presence of 33% of fetal bovine serum (FBS). In Egg-CaO solutions, except AIV, all pathogens were inactivated within 1 hr, even in the presence of 5% of FBS. Without FBS, all pathogens, except AIV, were inactivated within 3 min, and AIV within 1 hr. In addition, persistence of virucidal activity against AIV and NDV of Egg-CaO powder was confirmed after exposure to sunlight for 2 weeks or resuspension with water for 7 times, simulating field harsh environments. Chick growth test was conducted to ensure the safety of the use of Egg-CaO powder in chicken cages and showed that it is safe to add Egg-CaO in litter or feed. In conclusion, Egg-CaO can be useful for the enhancement of biosecurity at farms.

show abstract

Section: Discussionmentioning

confidence: 99%

“…RF=log 10 (amount of untreated intact pathogens/m l ) −log 10 (amount of treated pathogen with Egg-CaO/m l ). Inactivation was considered to be satisfied when RF was ≥3 [ 21 ].…”

Section: Methodsmentioning

confidence: 99%

Calcinated egg shell as a candidate of biosecurity enhancement material

Ota

Toyofuku

Thammakarn

et al. 2016

The Journal of Veterinary Medical Science

Self Cite

View full text Add to dashboard Cite

show abstract

“…In the above equation, tpc is bacterial titer from an untreated sample in log 10 CFU/m l , whereas ta is the titer of recovered bacteria from treated samples. The inactivation rate was acceptable when the RF was greater than or equal to 3 [ 13 , 27 , 42 , 43 ].…”

Section: Methodsmentioning

confidence: 99%

Inactivation of bacteria on surfaces by sprayed slightly acidic hypochlorous acid water: <i>in vitro</i> experiments

Hakim

Alam

Sangsriratanakul

et al. 2016

The Journal of Veterinary Medical Science

Self Cite

View full text Add to dashboard Cite

The capacity of slightly acidic hypochlorous acid water (SAHW), in both liquid and spray form, to inactivate bacteria was evaluated as a potential candidate for biosecurity enhancement in poultry production. SAHW (containing 50 or 100 ppm chlorine, pH 6) was able to inactivate Escherichia coli and Salmonella Infantis in liquid to below detectable levels (≤2.6 log10 CFU/ml) within 5 sec of exposure. In addition, SAHW antibacterial capacity was evaluated by spraying it using a nebulizer into a box containing these bacteria, which were present on the surfaces of glass plates and rayon sheets. SAHW was able to inactivate both bacterial species on the glass plates (dry condition) and rayon sheets within 5 min spraying and 5 min contact times, with the exception of 50 ppm SAHW on the rayon sheets. Furthermore, a corrosivity test determined that SAHW does not corrode metallic objects, even at the longest exposure times (83 days). Our findings demonstrate that SAHW is a good candidate for biosecurity enhancement in the poultry industry. Spraying it on the surfaces of objects, eggshells, egg incubators and transport cages could reduce the chances of contamination and disease transmission. These results augment previous findings demonstrating the competence of SAHW as an anti-viral disinfectant.

show abstract

“…There have been several trials to explain alternative materials for biosecurity enhancement, which do not interfere, even with contaminated organic materials. Several researchers have shown alkaline agents can inactivate bacteria, even in the presence of organic materials, such as bioceramic powder [26], slaked lime, scallop shell powder [28], calcinated egg shell [14], calcium hydroxide [20] and fresh charcoal ash [19]. However, several acidic agents, such as potassium monopersulfate, can inactivate both bacteria and viruses, even in the presence of organic material [22,23].…”

mentioning

confidence: 99%