Efficacy of Sakacin on Selected Food Pathogenic Microorganisms Isolated from Fermented Milk Products

Mohammed, Shamsudeen; Damisa, D; Balogu, T.V.; Bălă, Elena Cristina

doi:10.4314/jasem.v20i1.12

Cited by 2 publications

(2 citation statements)

References 5 publications

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“…The antibacterial activity of the BCEs were determined using agar well diffusion method described by Mohammed et al, (2016) with slight modifications. From each BCEs, 10mL was collected and labelled as 1.0 Au/mL, and three sequential dilutions (0.75 AU/mL, 0.5 AU/mL, 0.25 AU/mL) were made from it.…”

Section: Antibacterial Assaymentioning

confidence: 99%

“…Bacteriocins (Nisin) status as GRAS (Mohammed et al, 2016), have increased detailed studies on the potency and application of other bacteriocin as natural alternative substance for food preservations. Successful antimicrobial potencies of bacteriocins have been recorded (Mohammed et al, 2016;Gao et al 2010). Most bacteriocins are progressively studied either as crude extract form, semi purified or purified form via culture sediments, precipitation (ammonium sulfate, methanol extraction) and chromatographic activity.…”

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confidence: 99%

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Cultivation, isolation and characterization of bacteriocin from fresh cow milk and meat samples obtained from Lapai Market in Niger State Nigeria

Balogu¹,

J²,

Abdulsalam³

2017

Journal of Applied Sciences and Environmental Management

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This study focus on cultivation, isolation and characterization of Bacteriocin from fresh cow milk (FCM) and fresh cow meat (FMS) samples obtained from Lapai Market in Niger State, Nigeria. Potential bacteriocinogenic bacteria were screened with agar diffusion method on culture plates seeded with Staphylococcus and E.coli. Bacteriocinogenic isolates from FCM were Enterococcus faeciumFCM5 and Bacillus megaterium FCM6, while isolates from FMS include Bacillusbadius FMS1 and Micrococcus varians FMS2. Bacteriocins produced were assayed with pre-enriched MRS broth culture incubated at 30 0 C with adjusted pH (7.1) for 48 hrs and stored (4 0 C). The primed cultures were centrifuged (20,000rpm) for 30mins, supernatant were filtered (0.2µm membrane filters), precipitated with ammonium sulphate and steam sterilized (121 0 C at 15psi) for 15mins. Bacteriocin-like products (BLPs) were crystallized and tagged enterocin FCM5, bacillocin FCM6, bacillocin FMS1 and micrococin FMS2 according to bacteriocinogenic isolates' typing. After 48 hrs, Bacteriocinogenic load ranged between 5.1 -6.13Log10 cfu/mL. Biopreservative indexes (BI) of BLPs (0.25 -0.75 mg/mL) were effective against predominant food spoilers (Saccharomyces, Pseudomonas, Klebsiellaand Micrococcusspp isolated from range of beverages, starchy and meaty meals). Quantified BLPs (20 -1880 IU/mL) strengths had no direct correlation with the antibacterial spectral (6 -19mm) expressed as zone of inhibition. Thus, efficacy of BLPs observed in this study, were not a function of BLP quantity but quality. Conclusively,Enterocin FCM5, Bacilliocins (FCM6 and FMS1) and Micrococin FMS2 are thermal and pressure stable BLPs that are effective against predominant food spoilers. ©JASEM https://dx.doi.org/10.4314/jasem.v21i3.2

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Section: Antibacterial Assaymentioning

confidence: 99%

mentioning

confidence: 99%

Cultivation, isolation and characterization of bacteriocin from fresh cow milk and meat samples obtained from Lapai Market in Niger State Nigeria

Balogu¹,

J²,

Abdulsalam³

2017

Journal of Applied Sciences and Environmental Management

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A review of microbes and chemical contaminants in dairy products in sub‐Saharan Africa

Akinyemi

Ayeni

Ogunremi

et al. 2021

Comp Rev Food Sci Food Safe

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Animal milk types in sub‐Saharan Africa (SSA) are processed into varieties of products using different traditional methods and are widely consumed by households to support nutritional intake and diet. Dairy products contain several microorganisms, their metabolites, and other chemical compounds, some with health benefits and many others considered as potential health hazards. Consumption of contaminated milk products could have serious health implications for consumers. To access the safety of milk products across SSA, studies in the region investigating the occurrences of pathogens as well as chemical compounds such as heat stable toxins and veterinary drug residues in animal milk and its products were reviewed. This is done with a holistic view in light of the emerging exposome paradigm for improving food safety and consumer health in the region. Herein, we showed that several published studies in SSA applied conventional and/or less sensitive methods in detecting microbial species and chemical contaminants. This has serious implications in food safety because the correct identity of a microbial species and accurate screening for chemical contaminants is crucial for predicting the potential human health effects that undermine the benefits from consumption of these foods. Furthermore, we highlighted gaps in determining the extent of viral and parasitic contamination of milk products across SSA as well as investigating multiple classes of chemical contaminants. Consequently, robust studies should be conducted in this regard. Also, efforts such as development cooperation projects should be initiated by all stakeholders including scientists, regulatory agencies, and policy makers to improve the dairy product chain in SSA in view of safeguarding consumer health.

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Efficacy of Sakacin on Selected Food Pathogenic Microorganisms Isolated from Fermented Milk Products

Cited by 2 publications

References 5 publications

Cultivation, isolation and characterization of bacteriocin from fresh cow milk and meat samples obtained from Lapai Market in Niger State Nigeria

Cultivation, isolation and characterization of bacteriocin from fresh cow milk and meat samples obtained from Lapai Market in Niger State Nigeria

A review of microbes and chemical contaminants in dairy products in sub‐Saharan Africa

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