Efficacy of Potentially Probiotic Fruit-Derived Lactobacillus fermentum, L. paracasei and L. plantarum to Remove Aflatoxin M1 In Vitro

Abstract

This study evaluated the efficacy of potentially probiotic fruit-derived Lactobacillus isolates, namely, L. paracasei 108, L. plantarum 49, and L. fermentum 111, to remove aflatoxin M1 (AFM1) from a phosphate buffer solution (PBS; spiked with 0.15 µg/mL AFM1). The efficacy of examined isolates (approximately 109 cfu/mL) as viable and non-viable cells (heat-killed; 100 °C, 1 h) to remove AFM1 was measured after 1 and 24 h at 37 °C. The recovery of AFM1 bound to bacterial cells after washing with PBS was also evaluated. Levels of AFM1 in PBS were measured with high-performance liquid chromatography. Viable and non-viable cells of all examined isolates were capable of removing AFM1 in PBS with removal percentage values in the range of 73.9–80.0% and 72.9–78.7%, respectively. Viable and non-viable cells of all examined Lactobacillus isolates had similar abilities to remove AFM1. Only L. paracasei 108 showed higher values of AFM1 removal after 24 h for both viable and non-viable cells. Percentage values of recovered AFM1 from viable and non-viable cells after washing were in the range of 13.4–60.6% and 10.9–47.9%, respectively. L. plantarum 49 showed the highest AFM1 retention capacity after washing. L. paracasei 108, L. plantarum 49, and L. fermentum 111 could have potential application to reduce AFM1 to safe levels in foods and feeds. The cell viability of examined isolates was not a pre-requisite for their capacity to remove and retain AFM1.

Effects of non‐viableLactobacillus reutericombining with 14‐day standard triple therapy onHelicobacter pylorieradication: A randomized double‐blind placebo‐controlled trial

“…18 Increasing evidence has shown that in addition to living probiotics, dead cells, or even cell fractions may also exert biological effects. [19][20][21][22] In view of this, non-viable probiotics could be a safe alternative and have some advantages over viable probiotics, such as product stability and standardization. 19 L. reuteri DSM17648 strain was found by screening hundreds of Lactobacillus strains of a large culture collection.…”

Lactiplantibacillus plantarum from Unexplored Tunisian Ecological Niches: Antimicrobial Potential, Probiotic and Food Applications

“…Lactiplantibacillus plantarum (Lpb. plantarum) is a LAB with a long history of protechnological use in the food sector, in food fermentations, and for the design of protective cultures [13,14]. This species can adapt to a variety of niches and is widely distributed in the environment, i.e., it can be found in dairy products, in the gastrointestinal tracts of humans and animals, and in meat, fish, and fermented vegetables [15].…”

Mycotoxins: Toxicology, Identification and Control

“…One important point in control of mycotoxins is decontamination strategies, and in this sense, Oliveria da Cruz et al (2021) [7] evaluated the efficacy of potentially probiotic fruit-derived Lactobacillus isolates to remove aflatoxin M 1 (AFM 1 ) from a phosphate buffer solution (PBS; spiked with 0.15 µg/mL AFM 1 ). The authors concluded that L. paracasei 108, L. plantarum 49, and L. fermentum 111 could have potential application to reduce AFM 1 to safe levels in foods and feeds.…”