Efficacy of polymer coating of probiotic beads suspended in pressurized and pasteurized longan juices on the exposure to simulated gastrointestinal environment

Abstract

Alginate-coated Lactobacillus acidophilus LA5 or Lactobacillus casei 01 was recoated with either 0.1-0.5% (w/v) alginate or 0.05-0.15% (w/v) poly-L-lysine (PLL) plus 0.2% (w/v) alginate or 5-15% (w/v) gelatin, after which they were determined for survivability in gastric or bile longan juices. The morphology of encapsulated probiotic cells illustrated that recoated beads with 0.5% alginate showed a more compact surface and a greater protective effect than other recoating materials. The recoated beads with 0.5% alginate and 0.05-0.15% PLL plus 0.2% alginate of both strains showed the highest viability in gastric longan juice. In bile longan juice, only 0.5% alginate showed the best protection for both recoated beads. When considering the storage stability, encapsulated L. acidophilus LA5 exhibited a higher viable count than those of the free cells, whereas L. casei 01 showed equivalent viability of both free and double-coated cells. Based on the impact of pressurization or pasteurization, both processed juices gave rise to equivalent survivability of the probiotic cells during storage.

Spray drying Lactobacillus casei 01 in lychee juice varied carrier materials

“…A weight of 10 g dried probiotic cells were rehydrated in 100 ml de Man Rogosa and Sharpe (MRS) broth (HiMedia, Mumbai, India) for 10 min at room temperature and was incubated anaerobically at 37 C for 24 h using Anaerocult ® C system (Merck, Munich, Germany). Then, 1% (v/v) culture was inoculated in MRS broth and incubated anaerobically at 37 C for 14 h to achieve its early stationary stage (Chaikham, Apichartsrangkoon, Jirarattanarangsri, & Van de Wiele, 2012;Chaikham, Apichartsrangkoon, George, & Jirarattanarangsri, 2013). After the incubation, probiotic cells were harvested and washed twice with 0.85% (w/v) sterile saline water by centrifugation at 4000 Â g for 15 min at 4 C. The cell pallet was diluted to provide a bacterial concentration of 10 11 CFU/ml by saline water.…”

Evaluation of the Effect of Psyllium on the Viability of Lactobacillus Acidophilus in Alginate-Polyl Lysine Beads

“…Incorporation of poly l lysine on the alginate beads as the second layer and its effectiveness in the probiotics coating has also been studied. 30 …”

Plant Extracts in Probiotic Encapsulation: Evaluation of their Effects on Strains Survivability in Juice and Drinkable Yogurt During Storage and an in-vitro Gastrointestinal Model

“…Probiotic capsules were produced with some modifications in accordance with the procedure described by Chaikham, et al 12 . Cells were mixed with Sodium alginate sterile solution (Sigma-Aldrich, UK) and plant extracts were added in different concentrations (0.05-0.2%, w/v).…”

“…Encapsulation has been shown to be an alternative method for the safeguarding of probiotics from detrimental environmental factors 12 . Sodium alginate iscommonly used for this purpose because of its simplicity, low cost and biocompatibility 13 .…”

“…Sodium alginate iscommonly used for this purpose because of its simplicity, low cost and biocompatibility 13 . Many researchers have reported that alginate combined with other materials, for example, Hi-maize starch 14 , inulin, galactooligosaccharides and fluctooligosaccharides 15,16 , gelatine 12 , chitosan, pectin, and glucomannan 17,18 , can be used to improve the survival of different probiotic strains under gastrointestinal environments and in food products during storage.…”