EFFICACY OF PHEROMONE TRAPPING AND AERIAL SPRAYING OF Bacillus thuringiensis (Bt) FOR CONTROLLING BAGWORM, Metisa plana WALKER (Lepidoptera: Psychidae) IN YONG PENG, JOHOR, MALAYSIA

Ahmad, Mohd Najib

doi:10.21894/jopr.2017.2901.06

Cited by 10 publications

(7 citation statements)

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“…Control of O. rhinoceros by using entomopathogens is an alternative when the use of synthetic chemical insecticides is not able to solve pest problems. For example, Bacillus thuringiensis used to pest control has been tried to control Metisa plana caterpillars (Khaeruni and Purnamaningrum 2012;Ahmad et al 2017;), but to controlling O. rhinoceros is still not much studied.…”

Section: Introductionmentioning

confidence: 99%

Characteristics of Bacillus thuringiensis isolates indigenous soil of South Sumatra (Indonesia) and their pathogenicity against oil palm pests Oryctes rhinoceros (Coleoptera: Scarabaeidae)

2020

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Abstract. Pujiastuti Y, Arsi, Sandi S. 2020. Characteristics of Bacillus thuringiensis isolates indigenous soil of South Sumatra (Indonesia) and their pathogenicity against oil palm pests Oryctes rhinoceros (Coleoptera: Scarabaeidae). Biodiversitas 21: 1287-1294. Bacillus thuringiensis is a gram-positive, entomopathogenic bacterium that could be isolated from soil and be used to control various plant pests. Oryctes rhinoceros is an important pest in oil palm. Application of B. thuringiensis-based bioinsecticides is an alternative in controlling these pests. The purposes of this study were to isolate and identify B. thuringiensis bacteria from the soil of South Sumatra, production of B. thuringiensis-based bioinsecticides and to test their toxicity to O. rhinoceros larvae. The study was conducted in several cities/districts in the province of South Sumatra. Soil samples were taken from various habitats and B. thuringiensis isolates were grown on NGKG agar media. Among 76 soil samples (6 districts and 2 cities) B. thuringiensis colonies were obtained leading to 24 isolates of B. thuringiensis. Toxicity screening tests for armyworm Spodoptera litura were 55.79% (53 isolates) and their mortality to 25.26% O. rhinoceros larvae (24 isolates). From these isolates whose effectively killed O. rhinoceros larvae, 10 isolates were taken and propagated with Nutrient Broth (NB) and biourine enriched with 5% molasses. Number of spores produced was counted during 24, 48 and 72 hours. Furthermore, a bioassay test was carried out on O. rhinoceros larvae for 7 days. Isolate of KJ3P1 caused the highest mortality of O. rhinoceros larvae after 7 days of observation. SDS Page resulted in KJ3P1 and KJ3R5 isolates showing several bands whose content of various types of protein molecular weight. Isolation of B. thuringiensis in South Sumatra produced 2 isolates potentially to be active ingredients in production of bioinsecticides which were effective in killing O. rhinoceros larvae.

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Section: Introductionmentioning

confidence: 99%

Characteristics of Bacillus thuringiensis isolates indigenous soil of South Sumatra (Indonesia) and their pathogenicity against oil palm pests Oryctes rhinoceros (Coleoptera: Scarabaeidae)

2020

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“…Chemical insecticides application through trunk injection technique, ground spraying technique, and aerial spraying includes the use of trichlorfon, cypermethrin, methamidophos, and monocrotophos [ 14 ]. The use of biopesticides, such as Bacillus thuringiensis ( Bt ) [ 15 , 16 ], endoparasitoid [ 17 ], and pheromone mass trapping as a biological control [ 18 ] have been reported. Despite these efforts, effective control with economical benefits and safer choice is yet to be found.…”

Section: Introductionmentioning

confidence: 99%

The Developmental Transcriptome of Bagworm, Metisa plana (Lepidoptera: Psychidae) and Insights into Chitin Biosynthesis Genes

Rahmat

Zifruddin

Abidin³

et al. 2020

Genes

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Bagworm, Metisa plana (Lepidoptera: Psychidae) is a ubiquitous insect pest in the oil palm plantations. M. plana infestation could reduce the oil palm productivity by 40% if it remains untreated over two consecutive years. Despite the urgency to tackle this issue, the genome and transcriptome of M. plana have not yet been fully elucidated. Here, we report a comprehensive transcriptome dataset from four different developmental stages of M. plana, comprising of egg, third instar larva, pupa and female adult. The de novo transcriptome assembly of the raw data had produced a total of 193,686 transcripts, which were then annotated against UniProt, NCBI non-redundant (NR) database, Gene Ontology, Cluster of Orthologous Group, and Kyoto Encyclopedia of Genes and Genomes databases. From this, 46,534 transcripts were annotated and mapped to 146 known metabolic or signalling KEGG pathways. The paper further identified 41 differentially expressed transcripts encoding seven genes in the chitin biosynthesis pathways, and their expressions across each developmental stage were further analysed. The genetic diversity of M. plana was profiled whereby there were 21,516 microsatellite sequences and 379,895 SNPs loci found in the transcriptome of M. plana. These datasets add valuable transcriptomic resources for further study of developmental gene expression, transcriptional regulations and functional gene activities involved in the development of M. plana. Identification of regulatory genes in the chitin biosynthesis pathway may also help in developing an RNAi-mediated pest control management by targeting certain pathways, and functional studies of the genes in M. plana.

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“…Present studies are in correlation with the studies of Trissolcus a hymenopteran insect 26 . The studies on this insect have shown that virgin male Trissolcus have shown a positive response to Tetradecyl acetate, Ethyl (Z)-9-hexadecenoate GC-EAD components on exposure to EAG machine 27 . Present results also show that the number of Xanthopimpla trapped was more in the third crop than first and second crops.…”

Section: Rearing Of Anthereae Mylitta Drurymentioning

confidence: 99%

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2020

IJPSR

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Mating disruption by an artificial release of synthetic female pheromone components like Tetradecyl acetate (A), Ethyl (Z)-9hexadecenoate (B) and a mixture of these two at different concentrations is a feasible method to control Xanthopimpla predator. Male Xanthopimpla predator was evaluated for responses to these synthetic pheromone components in a laboratory wind tunnel and in Terminalia arjuna field. Septa loaded with different concentrations (0.2, 0.4, 0.6, 0.8 and 1.0 mg) of Tetradecyl acetate (A), Ethyl (Z)-9-hexadecenoate (B), A and B blend in 1:1 ratio (C) has disrupted the male Xanthopimpla predator orientation in the wind tunnel and blend with 1.0 mg had shown more attraction towards Xanthopimpla predator. Mating disruption efficacy in tasar field was evaluated in first, second and third crops with 16 traps in each crop. In all crops, 5 traps baited with pheromone loads of 0.2, 0.4, 0.6, 0.8 and 1.0 mg of Tetradecyl acetate, 5 traps with Ethyl (Z)-9-hexadecenoate and 5traps with a blend of both in 1:1 ratio. One blank control trap without any type of solvent is used in all three crops. Traps baited with a blend of 1.0 mg concentration caught more males than Tetradecyl acetate (A) and Ethyl (Z)-9hexadecenoate (B). The infestation rate is least in the cocoons reared on Terminalia plantation with traps baited with A and B blend (1.0 mg) followed by Ethyl (Z)-9-hexadecenoate (B) traps, Tetradecyl acetate (A) traps and control traps.

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EFFICACY OF PHEROMONE TRAPPING AND AERIAL SPRAYING OF Bacillus thuringiensis (Bt) FOR CONTROLLING BAGWORM, Metisa plana WALKER (Lepidoptera: Psychidae) IN YONG PENG, JOHOR, MALAYSIA

Abstract: Recurring bagworm outbreak is a major problem in the oil

Cited by 10 publications

References 1 publication

Characteristics of Bacillus thuringiensis isolates indigenous soil of South Sumatra (Indonesia) and their pathogenicity against oil palm pests Oryctes rhinoceros (Coleoptera: Scarabaeidae)

Characteristics of Bacillus thuringiensis isolates indigenous soil of South Sumatra (Indonesia) and their pathogenicity against oil palm pests Oryctes rhinoceros (Coleoptera: Scarabaeidae)

The Developmental Transcriptome of Bagworm, Metisa plana (Lepidoptera: Psychidae) and Insights into Chitin Biosynthesis Genes

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