Efficacy of pectin-honey hydrogel and vitamin E in prevention of post-surgical intraperitoneal adhesions in rabbits

Abstract

Abdominal as well as intra-visceral adhesions are considered to be one of the major postsurgical complications which is the results of inflammation, coagulation and fibrin depositions due to surgical injuries or irritations at the time of surgical intervention. In the present study, eighteen numbers of apparently healthy adult rabbits of either sex equally divided in to three groups were used to see the efficacy of Pectin-Honey Hydrogel and Olive oil-vitamin E preparation in prevention of induced intraperitoneal adhesions. Group A, Group B and Group C were treated with intraperitoneal application of normal saline, Pectin-Honey Hydrogel and Olive oil-vitamin E preparation respectively following induced peritonitis. Clinical parameters (0th 2nd, 4th, 6th, 8th and 10th days), biochemistry of peritoneal fluid (0th, 10th, 20th and 30th days) was carried out. The surgical wounds were reopened on 10th, 20th and 30th day for macroscopic assessment of adhesions. Clinical parameters found to be significantly elevated (P < 0.05) on 2nd post-surgical; however, the changes were within the physiological limit. Peritoneal fluid biochemistry revealed significant higher total protein concentration in Group A followed by Group C & B. A significantly lower catalase activity of peritoneal fluid was found in Group B post-surgically. Macroscopic peritoneal and visceral adhesion score was recorded highest in Group A and lowest in Group B. The present study revealed that Pectin-Honey Hydrogel and Olive oil-vitamin E preparation effectively prevent peritoneal adhesion in rabbits and promises its clinical use.