Efficacy of p16 and ProExC Immunostaining in the Detection of High-Grade Cervical Intraepithelial Neoplasia and Cervical Carcinoma

Guo, Ming; Baruch, Amy C.; Silva, Elvio G.; Jan, Yee Jee; Lin, E.; Sneige, Nour; Deavers, Michael T.

doi:10.1309/ajcp1llx8qmdxhho

Cited by 61 publications

(33 citation statements)

References 25 publications

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“…There is evidence to suggest that a negative feedback mechanism controlling p16 levels in normal cells is disrupted by a reduction of pRb activity in proliferating squamous epithelial cells expressing HR-HPV E7 (16). With regard to p16, it has been demonstrated that almost 100% of cases of HSIL and SCC associated with HR-HPV express high levels of p16, whereas non-dysplastic cervical epithelium or low-grade SIL (LSIL) associated with low-risk (LR) or negative HPV types do not express p16 (17). Ki-67 is a nuclear protein and is detected by mindbomb E3 ubiquitin protein ligase-1 (MIB-1), a monoclonal antibody that is associated with RNA transcription and cell cycle progression (18).…”

Section: Introductionmentioning

confidence: 99%

Efficacy of p16 and Ki-67 immunostaining in the detection of squamous intraepithelial lesions in a high-risk HPV group

et al. 2015

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Abstract. p16 and Ki-67 immunohistochemistry can be used as an ancillary method for the diagnosis of squamous intraepithelial lesion (SIL) versus atrophic change and atypical squamous metaplasia. The aim of the present study was to evaluate the efficacy of these two immunohistochemical markers in the accurate interpretation of cervical biopsies and correlate this data with human papilloma virus (HPV) infection status. The study included 103 formalin-fixed cervical punch and cone biopsy samples, with corresponding HPV DNA test data. Histopathological staining with hematoxylin and eosin, and immunohistochemical staining for p16 and Ki-67 were reviewed by two pathologists. The positivity of p16 and Ki-67 increased significantly with the severity of the cervical lesion in patients with a high-risk-HPV (HR-HPV) infection status (P<0.001). However, there was discordance in the HPV-negative group. Furthermore, concomitant diffuse, strong and block positive staining of p16, and a high Ki-67 index were implicated in high-grade SIL in the HR-HPV group. Thus, the two markers were efficient in advancing the diagnostic accuracy of cervical biopsies in the HR-HPV group; however, application of immunohistochemical results should be carefully considered in the HPV-negative group.

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Section: Introductionmentioning

confidence: 99%

Efficacy of p16 and Ki-67 immunostaining in the detection of squamous intraepithelial lesions in a high-risk HPV group

et al. 2015

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“…ProEx C positive expression seems to be more specifically associated with SIL than Ki-67 positive expression (Conesa-Zamora et al, 2009). ProExC immunostaining, when compared with p16 immunostaining, have similar specificity for CIN 2+ and higher specificity for CIN 3+ but lower sensitivity for CIN 2+ and CIN 3+ (Guo et al, 2011). Some reports suggested that ProExC can be more selective and informative for the progression of low-grade (CIN1) and moderate-grade (CIN2) lesions than Ki67 (Beccati et al, 2008).…”

Section: Proliferation Markersmentioning

confidence: 87%

The Indicators of Predicting Disease Outcome in HPV Carcinogenesis

Bleoţu¹,

Anton²

2012

Topics on Cervical Cancer With an Advocacy for Prevention

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“…p16 and vimentin (32,33). Previous studies found that p16INK4a had failed in distinguishing ECa and its expression was also related to a carcinogenesis mechanism involving HPV infection.…”

Section: Discussionmentioning

confidence: 99%

Panel of villin, pro-ex-c, estrogen receptor and progesterone receptor expressions could help in differentiation between endocervical and endometrioid adenocarcinoma

Elfeky

Harb²,

Gertallah³

2017

TJPATH

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Objective: Endocervical and endometrioid adenocarcinoma have marked overlapping features and the differentiation between them is important for their accurate management. Villin is an actin-binding protein which has an important role in the maintenance of microvilli in epithelial cells and epithelial cell-specific anti-apoptotic protein processes. Pro-Ex-C is a marker for higher-risk human papilloma virus (HPV) which targets the cell cycle proteins causing their overexpression. the aim of the study was to clarify the diagnostic and predictive role of villin, Pro-Ex-C, estrogen receptor (ER) and progesterone receptor (PR) expression in endocervical and endometrioid adenocarcinoma. Material and Method:We evaluated villin, Pro-Ex-C, ER and PR expressions in 15 cases of endocervical adenocarcinoma and 30 cases of endometrioid adenocarcinoma. We analyzed the diagnostic and predictive role of that panel in both carcinoma subtypes. Sensitivity, specificity, positive predictive value, negative predictive value, and accuracy were calculated. Results:Positive villin and Pro-Ex-C expressions were positively correlated with the presence and pattern of cervical stromal invasion (p<0.05). ER was positive in all cases of endometrioid adenocarcinoma. PR was detected in most cases of endometrioid adenocarcinoma. the differences of villin, Pro-Ex-C, ER and PR expression in endocervical and endometrioid adenocarcinoma was statistically significant (p<0.05). this methodology for distinguishing endocervical and endometrioid adenocarcinoma had a sensitivity of 100%, a specificity of 100% and a significant prognostic and predictive role.

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Efficacy of p16 and ProExC Immunostaining in the Detection of High-Grade Cervical Intraepithelial Neoplasia and Cervical Carcinoma

Cited by 61 publications

References 25 publications

Efficacy of p16 and Ki-67 immunostaining in the detection of squamous intraepithelial lesions in a high-risk HPV group

Efficacy of p16 and Ki-67 immunostaining in the detection of squamous intraepithelial lesions in a high-risk HPV group

The Indicators of Predicting Disease Outcome in HPV Carcinogenesis

Panel of villin, pro-ex-c, estrogen receptor and progesterone receptor expressions could help in differentiation between endocervical and endometrioid adenocarcinoma

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