Efficacy of ozone on survival and permeability of oral microorganisms

Abstract

In the present study, we examined the effect of ozonated water on oral microorganisms and dental plaque. Almost no microorganisms were detected after being treated with ozonated water (4 mg/l) for 10 s. To estimate the ozonated water-treated Streptococcus mutans, bacterial cells were stained with LIVE/DEAD BacLight Bacterial Viability Kit. Fluorescence microscopic analysis revealed that S. mutans cells were killed instantaneously in ozonated water. Some breakage of ozonated water-treated S. mutans was found by electron microscopy. When the experimental dental plaque was exposed to ozonated water, the number of viable S. mutans remarkably decreased. Ozonated water strongly inhibited the accumulation of experimental dental plaque in vitro. After the dental plaque samples from human subjects were exposed to ozonated water in vitro, almost no viable bacterial cells were detected. These results suggest that ozonated water should be useful in reducing the infections caused by oral microorganisms in dental plaque.

Aqueous-ozone irrigation of bone monocortical wounds in hyperglycemic rats

“…At this concentration, no harmful effects to bone healing could be seen. This concentration of aqueous ozone (0,004mg/ml) was based on other studies that established in vitro antibacterial activity for similar concentrations (0,002 -0,004mg/ml) 16,17 .…”

The antimicrobial effects of root canal irrigation and medication

“…77 Ozonated water has also been investigated as a root canal irrigant. Ozone is known to act as a strong antimicrobial agent 78 and has been shown to effectively disinfect bovine dentinal tubules in vivo. 79 These developments in the field of root canal disinfection provide encouraging results for future development of novel irrigation materials and techniques that can be used instead of or as adjunct to the conventional materials currently in use.…”

The antimicrobial effect of 0.1 ppm ozonated water on 24-hour plaque microorganisms in situ

“…The reduction in the CFU counts in this group may be attributed to the mechanical dislodging or "washing away" of loose supragingival plaque that formed within the 24-hour period or to the inactivation of viable bacteria by the distilled water. A decrease in the cell viability of bacteria has been reported 23 for bacteria grown on agar plates and treated with distilled water. The finding that post-rinse samples isolated from the group treated with distilled water exhibited more CFUs compared to the pre-rinse samples could be due to differences in the bacterial concentrations in the plaque samples collected from the different quadrants of the upper and lower teeth.…”

“…20 Ozonated water has been used in the sterilization of dentures (10 ppm) 21 and dental unit water-line systems. 22 Plaque microorganisms have shown vulnerability to ozonated water under invitro conditions 23 (4 mg/l for 10 seconds). The main objective of this study was to determine the in situ antimicrobial effect of 0.1 ppm ozonated water on 24-hour plaque microorganisms following a 30-second rinse.…”