Efficacy of Octenidine Hydrochloride for Reducing Escherichia coli O157:H7, Salmonella spp., and Listeria monocytogenes on Cattle Hides

Baskaran, Sangeetha Ananda; Upadhyay, Abhinav; Upadhyaya, Indu; Bhattaram, Varunkumar; Venkitanarayanan, Kumar

doi:10.1128/aem.00259-12

Cited by 10 publications

(3 citation statements)

References 34 publications

(15 reference statements)

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“…coli O157:H7, Salmonella Enteritidis, Acinetobacter baumannii , Candida albicans and Fusobacterium nucleatum , S . aureus and Pseudomonas aeruginosa [ 13 , 14 , 15 , 16 , 17 ]. Toxicity studies in a variety of species have shown that OH is not absorbed through the mucous membrane and gastrointestinal tract, with no reported carcinogenicity, genotoxicity or mutagenicity [ 18 ].…”

Section: Introductionmentioning

confidence: 99%

Antibiofilm Effect of Octenidine Hydrochloride on Staphylococcus aureus, MRSA and VRSA

Amalaradjou

Venkitanarayanan

2014

Pathogens

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Millions of indwelling devices are implanted in patients every year, and staphylococci (S. aureus, MRSA and vancomycin-resistant S. aureus (VRSA)) are responsible for a majority of infections associated with these devices, thereby leading to treatment failures. Once established, staphylococcal biofilms become resistant to antimicrobial treatment and host response, thereby serving as the etiological agent for recurrent infections. This study investigated the efficacy of octenidine hydrochloride (OH) for inhibiting biofilm synthesis and inactivating fully-formed staphylococcal biofilm on different matrices in the presence and absence of serum protein. Polystyrene plates and stainless steel coupons inoculated with S. aureus, MRSA or VRSA were treated with OH (zero, 0.5, one, 2 mM) at 37 °C for the prevention of biofilm formation. Additionally, the antibiofilm effect of OH (zero, 2.5, five, 10 mM) on fully-formed staphylococcal biofilms on polystyrene plates, stainless steel coupons and urinary catheters was investigated. OH was effective in rapidly inactivating planktonic and biofilm cells of S. aureus, MRSA and VRSA on polystyrene plates, stainless steel coupons and urinary catheters in the presence and absence of serum proteins. The use of two and 10 mM OH completely inactivated S. aureus planktonic cells and biofilm (>6.0 log reduction) on all matrices tested immediately upon exposure. Further, confocal imaging revealed the presence of dead cells and loss in biofilm architecture in the OH-treated samples when compared to intact live biofilm in the control. Results suggest that OH could be applied as an effective antimicrobial to control biofilms of S. aureus, MRSA and VRSA on appropriate hospital surfaces and indwelling devices.

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Section: Introductionmentioning

confidence: 99%

Antibiofilm Effect of Octenidine Hydrochloride on Staphylococcus aureus, MRSA and VRSA

Amalaradjou

Venkitanarayanan

2014

Pathogens

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“…sanguis ( Bailey et al, 1984 ). Recent studies have also demonstrated its antimicrobial efficacy against Escherichia coli O157:H7 (OH @ 0.15 and 0.25%), Staphylococcus aureus , Methicillin-resistant and Vancomycin-resistant S. aureus (MRSA, VRSA; OH @ 0.15, 0.3 and 0.6%), Salmonella Enteritidis (OH @ 0.15 and 0.25%), Enterococcus faecalis (OH @ 0.1%), Candida albicans (OH @ 0.1%) Listeria monocytogenes (OH @ 0.15 and 0.25%) and Pseudomonas aeruginosa (OH @ 0.1%; Zumbotel et al, 2009 ; Tirali et al, 2010 ; Baskaran et al, 2012 ; Amalaradjou and Venkitanarayanan, 2014 ; Junka et al, 2014 ; Makkar et al, 2015 ). OH was found to possess high tissue tolerability, and hence has been applied topically to prevent open wound infections ( Hubner et al, 2010 ; Vanscheidt et al, 2012 ).…”

Section: Introductionmentioning

confidence: 99%

Inactivation of Acinetobacter baumannii Biofilms on Polystyrene, Stainless Steel, and Urinary Catheters by Octenidine Dihydrochloride

et al. 2016

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Acinetobacter baumannii is a major nosocomial pathogen causing human infections with significant mortality rates. In most cases, infections are acquired through exposure to A. baumannii biofilms that persist on contaminated hospital equipment and surfaces. Thus, it is imperative to develop effective measures for controlling A. baumannii biofilms in nosocomial settings. This study investigated the efficacy of octenidine dihydrochloride (OH), a new generation disinfectant for reducing A. baumannii biofilms on polystyrene, stainless steel and catheters. OH at 0.3% (5 mM), 0.6% (10 mM), and 0.9% (15 mM) was effective in significantly inactivating A. baumannii biofilms on all tested surfaces (P < 0.05). Furthermore, OH was equally effective in inactivating biofilms of multidrug resistant and drug susceptible A. baumannii isolates. In addition, confocal imaging revealed the predominance of dead cells in the OH-treated samples in comparison to the control. Further, scanning electron microscopy of biofilms formed on catheters revealed that OH treatment significantly reduced A. baumannii biofilm populations in corroboration with our antibiofilm assay. These data underscore the efficacy of OH in inactivating A. baumannii biofilms, thereby suggesting its potential use as a disinfectant or a catheter lock solution to control A. baumannii infections.

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“…OCT, approved as a medicinal substance in several European countries, is an established bispyridine antiseptic with a broad activity and is commonly used as wound antiseptic [ 23 , 24 ]. It has attracted increasing attentions because of broad antibacterial spectrum including antibiotic-resistant bacteria, noncytotoxicity at suitable doses, satisfactory stability, and smaller possibility to develop resistant bacteria [ 25 , 26 ].…”

Section: Introductionmentioning

confidence: 99%

Antibacterial Effects and Biocompatibility of Titania Nanotubes with Octenidine Dihydrochloride/Poly(lactic-co-glycolic acid)

Lai²,

et al. 2015

BioMed Research International

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Titanium (Ti) implants with long-term antibacterial ability and good biocompatibility are highly desirable materials that can be used to prevent implant-associated infections. In this study, titania nanotubes (TNTs) were synthesized on Ti surfaces through electrochemical anodization. Octenidine dihydrochloride (OCT)/poly(lactic-co-glycolic acid) (PLGA) was infiltrated into TNTs using a simple solvent-casting technique. OCT/PLGA-TNTs demonstrated sustained drug release and maintained the characteristic hollow structures of TNTs. TNTs (200 nm in diameter) alone exhibited slight antibacterial effect and good osteogenic activity but also evidently impaired adhesion and proliferation of bone marrow mesenchymal stem cells (BMSCs). OCT/PLGA-TNTs (100 nm in diameter) supported BMSC adhesion and proliferation and showed good osteogenesis-inducing ability. OCT/PLGA-TNTs also exhibited good long-term antibacterial ability within the observation period of 7 d. The synthesized drug carrier with relatively long-term antibacterial ability and enhanced excellent biocompatibility demonstrated significant potential in bone implant applications.

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Efficacy of Octenidine Hydrochloride for Reducing Escherichia coli O157:H7, Salmonella spp., and Listeria monocytogenes on Cattle Hides

Abstract: ABSTRACTThe efficacy of octenidine hydrochloride (OH; 0.025, 0.15, and 0.25%) for inactivatingEscherichia coliO157:H7,Salmonellaspp., andListeria monocytogeneson cattle hides was investigated at 23°C in the presence and absence of bovine feces. All tested concentrations o… Show more

Cited by 10 publications

References 34 publications

Antibiofilm Effect of Octenidine Hydrochloride on Staphylococcus aureus, MRSA and VRSA

Antibiofilm Effect of Octenidine Hydrochloride on Staphylococcus aureus, MRSA and VRSA

Inactivation of Acinetobacter baumannii Biofilms on Polystyrene, Stainless Steel, and Urinary Catheters by Octenidine Dihydrochloride

Antibacterial Effects and Biocompatibility of Titania Nanotubes with Octenidine Dihydrochloride/Poly(lactic-co-glycolic acid)

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