Efficacy of novel 6-phytase from Buttiauxella sp. on ileal and total tract nutrient digestibility in growing pigs fed a corn-soy based diet

Velayudhan, Deepak; Heo, Jung Min; Dersjant-Li, Y.; Owusu-Asiedu, A.; Nyachoti, C. M.

doi:10.1016/j.anifeedsci.2015.10.005

Cited by 27 publications

(23 citation statements)

References 27 publications

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“…The quadratic decrease in the AID of GE that was observed as phytase was added to the diets is in contrast with previous data, which indicated no effect of phytase on ileal digestibility of energy (Liao et al, 2005;Cervantes et al, 2011;Zeng et al, 2015). However, in other studies, an increase in the AID of GE with supplementation of phytase was observed (Velayudhan, 2015;Kiarie et al, 2016). The negative effect of phytase on the ATTD of GE that was observed in this experiment is in agreement with a recent meta-analysis for effects of microbial phytase on ATTD of GE (Torres-Pitarch et al, 2017).…”

Section: Amino Acids and Energycontrasting

confidence: 96%

“…The observation that there was no effect of phytase on the AID of AA is in agreement with She et al (2018), who reported that inclusion of up to 4,000 FTU of an E. coli phytase to a corn-soybean meal-based diet had no impact on the AID of AA. Likewise, addition of up to 2,000 FTU of an E. coli phytase had no effect on the AID of the majority of AA (Liao et al, 2005;Velayudhan et al, 2015). These observations indicate that for diets based on corn and soybean meal, or corn, soybean meal, and canola meal, there appears to be no effect of phytase on the AID of AA by cannulated growing pigs.…”

Section: Amino Acids and Energymentioning

confidence: 81%

“…A linear effect of up to 2,000 FTU of phytase on the ATTD of P, but not on Ca, was also reported (Velayudhan et al, 2015). It is possible that the conflicting responses to increased dietary phytase among experiments may be the result of differences among experiments in the substrate for phytase, differences in the potency of the phytase used, or differences in the P sufficiency of the test diet.…”

Section: Calcium and Phosphorusmentioning

confidence: 85%

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Effects of microbial phytase on mucin synthesis, gastric protein hydrolysis, and degradation of phytate along the gastrointestinal tract of growing pigs1

Mesina

Lagos

Sulabo

et al. 2018

Journal of Animal Science

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An experiment was conducted to test the hypothesis that pigs fed diets supplemented with exogenous phytase reduce mucin synthesis in the small intestine, increase protein hydrolysis in the stomach, increase breakdown of phytate along the gastrointestinal tract, and increase mineral and AA digestibility. A diet based on corn, soybean meal, and canola meal was formulated to meet requirements for growing pigs except for Ca and P, which were lower than requirements. Three additional diets were formulated by adding 750, 1,500, or 3,000 units of phytase (FTU) per kilogram to the basal diet. Eight growing barrows (38.45 ± 3.06 kg) were prepared with a T-cannula in the duodenum and another T-cannula in the distal ileum. Pigs were housed individually and allotted to a replicated 4 × 4 Latin square design with four pigs and four periods in each square. Each period lasted 14 d with the initial 7 d being the adaptation period to the diets. Pigs were fed twice daily in combined amounts equal to 3.2 times the estimated requirement for maintenance energy. Results indicated that the apparent ileal digestibility (AID) and the apparent total tract digestibility (ATTD) of Ca and P increased (linear and quadratic, P ≤ 0.05)

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Section: Amino Acids and Energycontrasting

confidence: 96%

Section: Amino Acids and Energymentioning

confidence: 81%

Section: Calcium and Phosphorusmentioning

confidence: 85%

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Effects of microbial phytase on mucin synthesis, gastric protein hydrolysis, and degradation of phytate along the gastrointestinal tract of growing pigs1

Mesina

Lagos

Sulabo

et al. 2018

Journal of Animal Science

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“…Thus, the results of this experiment indicating that microbial phytase did not increase the ATTD of DM and GE or the DE in the diet are in agreement with previous data. However, data from other experiments indicated that phytase may improve the ATTD of GE (Adedokun et al, 2015;Velayudhan et al, 2015). It is possible that the different responses may be due to the use of different phytases or being a result of different Ca:P ratios in the diets, but research to address these hypotheses has not been reported.…”

Section: Apparent Ileal Digestibility Of Aamentioning

confidence: 98%

Effects of increasing concentrations of an Escherichia coli phytase on the apparent ileal digestibility of amino acids and the apparent total tract digestibility of energy and nutrients in corn-soybean meal diets fed to growing pigs1

She

Sparks²,

Stein

2018

Journal of Animal Science

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An experiment was conducted to test the hypothesis that inclusion of increasing concentrations of an Escherichia coli phytase to a corn-soybean meal (SBM) diet results in improved digestibility of DM, GE, CP, NDF, ADF, macrominerals, microminerals, and AA. Twenty-four growing barrows (initial BW: 37.0 ± 1.4 kg) were equipped with a T-cannula in the distal ileum and placed individually in metabolism crates, and allotted to a 2-period switch-back design with 6 diets and 4 replicate pigs per diet in each period. The positive control diet was a corn-SBM diet that contained limestone and dicalcium phosphate to meet the requirement for standardized total tract digestible (STTD) P and Ca (0.31% STTD P and 0.70% Ca). A negative control diet that was similar to the positive control diet, with the exception that no dicalcium phosphate was used, was also formulated, and this diet contained 0.16% STTD P and 0.43% Ca. Four additional diets were formulated by adding 500, 1,000, 2,000, or 4,000 units of microbial phytase (FTU) to the negative control diet. Each period lasted 14 d. Fecal and urine samples were collected from the feed provided from days 6 to 11 of each period following 5 d of adaptation to the diets. Ileal digesta were collected for 8 h on days 13 and 14. Results indicated that addition of the E. coli phytase to the negative control diet tended to quadratically improve the apparent ileal digestibility of Phe (P = 0.086) and Asp (P = 0.054), and linearly increased (P < 0.05) the apparent total tract digestibility (ATTD) of ADF, K, and Fe. Microbial phytase also quadratically increased (P < 0.05) the ATTD of NDF and Mg, and linearly and quadratically increased (P < 0.05) the ATTD and retention of Ca and P. However, no effects of the phytase on ATTD of GE or the concentration of DE were observed. In conclusion, the increased absorption of several minerals including Ca, P, K, Mg, and Fe that was observed as increasing concentrations of an E. coli phytase was added to a corn-SBM meal diet indicates that the dietary provision of these minerals may be reduced if phytase is fed.

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“…Phytase is an enzyme that liberates the Pi present in phytic acid and makes it available for digestion in animals (Vats et al, 2009;Velayudhan et al, 2015). When used as a supplement, it has been shown to reduce the Pi in manure by about 33%, which ensures a third less environmental pollution and improvement of animal nutrition.…”

Section: Introductionmentioning

confidence: 99%

Optimized production of phytase by solid-state fermentation using triticale as substrate and source of inducer

Neira-Vielma¹,

Cristobal²,

Anna³

et al. 2018

Afr. J. Biotechnol.

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This study was carried out to evaluate the process of phytase production by Aspergillus niger in solidstate fermentation (SSF) using triticale waste. A waste that currently has no use was reported for this biotechnological process, and is of high impact due to the null use. The process was carried out using an additive free medium, supplemented with only one nitrogen source. Under these conditions, phytase activity of 7.45 U/g dry substrate (DS) was obtained. The process was optimized using different additives such as dextrose, lactose, Tween 80 and potassium chloride. For fermentation maximization, two experimental designs were used: 1) Plackett-Burman design (PBD) and 2) the Box-Behnken design (BBD). PBD was used to evaluate the effect of related variables on the production of phytase, as well as their level of significance in the process, while BBD was used for optimal conditions determination. The process was conducted with Petri dishes and a maximum enzyme activity of 25.8 IU/g DS was obtained. Subsequently, SSF was carried out in a tray to increase the amount of fermented substrate and phytase activity of 23.63 IU/g DS was obtained. The results of this study suggest a minimal decrease (8.4%) in enzyme production with scaling.

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Efficacy of novel 6-phytase from Buttiauxella sp. on ileal and total tract nutrient digestibility in growing pigs fed a corn-soy based diet

Cited by 27 publications

References 27 publications

Effects of microbial phytase on mucin synthesis, gastric protein hydrolysis, and degradation of phytate along the gastrointestinal tract of growing pigs1

Effects of microbial phytase on mucin synthesis, gastric protein hydrolysis, and degradation of phytate along the gastrointestinal tract of growing pigs1

Effects of increasing concentrations of an Escherichia coli phytase on the apparent ileal digestibility of amino acids and the apparent total tract digestibility of energy and nutrients in corn-soybean meal diets fed to growing pigs1

Optimized production of phytase by solid-state fermentation using triticale as substrate and source of inducer

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