Efficacy of natural formulations in bovine mastitis pathology: alternative solution to antibiotic treatment

Pașca, Claudia; Mărghitaș, Liviu Al.; Dezmirean, Daniel Severus; Matei, Ioana Adriana; Bonta, Victoriţa; Pașca, I.; Chirilă, F.; Cîmpean, A.; Fiț, N.

doi:10.2478/jvetres-2020-0067

Cited by 17 publications

(14 citation statements)

References 20 publications

(34 reference statements)

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“…However, the widespread use of antibiotics has led to an increased risk of the emergence of allergic and drug-resistant pathogens, and poses ongoing risks to human health and food safety. Therefore, the need to develop alternative drugs and treatments for the dairy industry is urgent [ 26 ]. H 2 S, as a gaseous transmitter, is mainly synthesized by CTH, which has broad substrate specificity and can catalyze cysteine to pyruvate, ammonia and thiocysteine.…”

Section: Discussionmentioning

confidence: 99%

CTH/H2S Regulates LPS-Induced Inflammation through IL-8 Signaling in MAC-T Cells

Lin

Bai

Gao

et al. 2022

IJMS

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Hydrogen sulfide (H2S), as an endogenous gaseous signaling molecule, plays an important role in the inflammatory process. Our previous study found that Cystathionine-γ-lyase (CTH) and H2S are correlated with the occurrence and development of Clinical Mastitis (CM) in Holstein cows. However, the functions and regulatory mechanisms of CTH/H2S are still unknown. In this study, the inflammatory mammary cell model based on the MAC-T cell line was established by Lipopolysaccharide (LPS)-induced manner to further explore the function and regulatory mechanism of CTH/H2S in cows with CM. In the inflammatory MAC-T cell, the CTH expression and H2S production were both repressed in an LPS-dose dependent manner, which demonstrated that CTH/H2S is related to the progression of inflammation. The inhibition of CTH/H2S using a selective CTH inhibitor, β-cyano-L-Alanine (BCA), promoted LPS-induced inflammation response and the expression of inflammatory cytokines. However, this was reversed by the H2S donor NaHS, demonstrating that H2S can protect cells from inflammatory damage. Intriguingly, interleukin-8 (IL-8) showed an inverse expression pattern correlated with the H2S-mediated cell protection effect during the inflammation process, and the inhibition test using a selective IL-8 receptor antagonist, SB225002, showed that IL-8 signaling plays a critical role in mediating endogenous H2S synthesis, and CTH/H2S exerts its anti-inflammation via IL-8-mediated signaling. This study provided support for the prevention and treatment of CM and the development of a novel anti-inflammatory strategy.

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Section: Discussionmentioning

confidence: 99%

CTH/H2S Regulates LPS-Induced Inflammation through IL-8 Signaling in MAC-T Cells

Lin

Bai

Gao

et al. 2022

IJMS

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“…93 Similarly, the alcoholic extracts of marigold, absinthe wormwood, essential oils of oregano, lavender, and rosemary could be effective against mastitis. 6 Methanolic and ethanolic extracts of Laggera alata showed antibacterial activity against Streptococcus agalactiae and Staphylococcus aureus isolated from bovine mastitis. 94 Methanolic extracts of Gymnema sylvestre, Holarrhenaanti dysenterica, Vernonia anthelmintica, Enicostemma littorale, Momordica charantia, Swertia chirata, Azadirachta indica and Caesalpinia bonducella showed more or less antibacterial activity against mastitis pathogens.…”

Section: Medicinal Plants Used Against Mastitismentioning

confidence: 99%

“…5 High somatic cell count (SCC) in mastic cows is detected by the flurooptoelectronic method. 6 SCC approximately 200,000 cells/ml is the threshold value for mastitis. 7 Mastitis also can transmit chronic diseases like tuberculosis brucellosis, leptospirosis, etc.…”

Section: Introductionmentioning

confidence: 99%

Bovine Mastitis: Causes and Phytoremedies

Acharya¹,

Parida²,

Mohapatra³

et al. 2022

J. Pure Appl. Microbiol.

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Mastitis is a highly frequent chronic ailment with inflammation in the udder of the milking cows. The causative agents are mostly microbes. It is economically prominent contamination of lactating cows resulting in reduced milk production. The disease is diagnosed by chemical, physical and nutritional changes in the milk and pathological changes in the milk glands. Prevention measures for the disease can be taken by proper and timely sanitation of the cowshed through and time again disinfection of the teat, mechanized milking process, etc. The application of bactericidal drugs generates resistant varieties of microbes that cross the allopathic boundary. In this regard, an attempt is taken to focus the plant-based pharmacopoeia. Medicinal plants are traditionally used to cure various diseases as they are comparatively accessible to administer orally in different forms and can be along with fodder. Keeping the above facts in view, the present review deals with different types of mastitis, causative pathogens, detection and diagnosis, and effective plant-based treatment process available to date.

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“…Along with antimicrobial drug optimization, development of antimicrobial alternatives presents the core principles of AMS. Consequently, there is continuous pressure on veterinarians to reduce the use of antibiotics in the treatment of food-producing animals, which is leading to development of new agents with innovative chemistry and modes of action [ 19 , 21 , 22 ].…”

Section: Introductionmentioning

confidence: 99%

Intramammary Ethno-Veterinary Formulation in Bovine Mastitis Treatment for Optimization of Antibiotic Use

et al. 2023

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Increasing pressure on animal and human health as well as food security, due to antimicrobial resistance, is leading to increased demand for natural-product-derived antibiotics worldwide. Considering the importance of bovine mastitis as well as the huge challenge posed by mastitis therapy in cattle production, the main goal of this research was to evaluate the effectiveness of the essential oil-based pharmaceutical (Phyto-Bomat), as an alternative to the existing treatment with antibiotics. The therapeutic response of the cows was monitored using clinical and bacteriological cure of the proposed formulation. Among 550 dairy cows, 75 were diagnosed with a clinical or subclinical form of mastitis and divided into three experimental groups: treated with antibiotic; Phyto-Bomat; antibiotic and Phyto-Bomat. Indicators of bacteriological cure were somatic cell count (SCC), total bacterial count, and causative agent isolation, while the clinical cure was accessed by observing physical udder conditions. All mentioned indicators were assessed before and after the treatment (1st and 7th day post-treatment), with all of the three therapeutic protocols. After Phyto-Bomat treatment, no visible signs of irritation were noticed, while no significant effect in reducing SCC and total bacterial count was observed. Phyto-Bomat has shown promising results in the treatment of mastitis, without the risk of udder irritation, alone or in combination with antibiotics as part of a mastitis control program during lactation.

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Efficacy of natural formulations in bovine mastitis pathology: alternative solution to antibiotic treatment

Cited by 17 publications

References 20 publications

CTH/H2S Regulates LPS-Induced Inflammation through IL-8 Signaling in MAC-T Cells

CTH/H2S Regulates LPS-Induced Inflammation through IL-8 Signaling in MAC-T Cells

Bovine Mastitis: Causes and Phytoremedies

Intramammary Ethno-Veterinary Formulation in Bovine Mastitis Treatment for Optimization of Antibiotic Use

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