Efficacy of naringenin against permethrin‐induced testicular toxicity in rats

Abstract

SUMMARYPermethrin (PM), a synthetic pyrethroid insecticide, has broad toxicity spectra. We aimed to investigate the effects of PM on the testes of adult albino rats, examine the recovery response and evaluate the efficacy of naringenin (NG) supplementation. Adult male albino rats were randomly assigned to five groups of six each: control, NG (50 mg/kg), PM (70 mg/kg), recovery (after subsequent withdrawal of PM) and NG-PM group. All treatments were given by oral gavage for 6 weeks and another 3 weeks for the recovery group. At the time of sacrifice, each testis was weighed. Biochemical analysis of epididymal sperm count and serum testosterone level was performed. Testes were processed for histological, ultrastructural and c-Kit immunohistochemical study. PM toxicity was evidenced by a highly significant decrease in testicular weight, epididymal sperm count and serum testosterone level compared to control. Furthermore, testicular structure abnormalities and reduced c-Kit immunoreactions were observed. Stoppage of PM in the recovery group partially reversed PMinduced changes. There was a mild decrease in testicular weight and biochemical parameters compared to control. The structure of seminiferous tubules was partially retained. The NG-PM group showed an overall improvement in testicular weight and biochemical alterations which were confirmed by light and electron microscopic examination. In conclusion, PM induced testicular toxicity, which was ameliorated by NG co-administration. However, stoppage of PM exposure was associated with partial recovery.

Effect of crocin and naringenin supplementation in cryopreservation medium on post-thawed rooster sperm quality and expression of apoptosis associated genes

“…It is demonstrated that in stressful conditions, naringenin has the ability to chelate irons and decrease ROS production. Interestingly, it is related to the fact that naringenin has 5-hydroxy and 4-carbonyl groups in the C-ring which plays a role in ROS scavenging, Cu, and Fe ions interaction [38, 39]. Therefore, adding naringenin to the cryopreservation medium can decrease the stress caused by freezing, consequently can increase motility which was observed in our study.…”

Naringenin attenuates highly active antiretroviral therapy‐induced spermDNAfragmentations and testicular toxicity in Sprague‐Dawley rats

“…This was further seen in the combination treatment, where the sperm counts were decreased compared to the control but higher in comparison to HAART only and Naringenin only treatments. Under stress condition, Naringenin has the potential to chelate irons and scavenge ROS (Mostafa et al ., ). 5‐hydroxy and 4‐carbonyl groups in the C‐ring of Naringenin play a role in ROS scavenging, Cu, and Fe ions interaction.…”

“…5‐hydroxy and 4‐carbonyl groups in the C‐ring of Naringenin play a role in ROS scavenging, Cu, and Fe ions interaction. Naringenin also restores mitochondrial membrane potential mitigating mitochondrial dysfunction and subsequent apoptotic cascade (Mostafa et al ., ). This could possibly explain the higher sperm count seen in HAART combined with Naringenin groups compared to HAART, and Naringenin treated groups as Naringenin mitigated the mitochondrial dysfunction and stress induced by HAART exposure.…”

Spermatogenesis induction of spermatogonial stem cells using nanofibrous poly(l‐lactic acid)/multi‐walled carbon nanotube scaffolds and naringenin

“…Among antioxidant components used for male reproductive systems, there is an evidence that naringenin, a well‐known flavonoid, has a considerable influence on the health of testicular cells and can act as a radical scavenger, an immunity system modulator, and an anti‐inflammatory factor . Roy et al demonstrated that narigenin can modulate the levels of ROS and improves diabetes‐induced testicular dysfunction in diabetic rats .…”