Efficacy of motile sperm organelle morphology examination (MSOME) for predicting pregnancy after intrauterine insemination

Akl, Lívia Drumond; Oliveira, J.B.A.; Petersen, C.G.; Mauri, A.L.; Baruffi, R. L. R.; Franco, J.G.

doi:10.1016/j.fertnstert.2011.07.1010

Cited by 4 publications

(4 citation statements)

References 47 publications

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“…In the absence of consensus on 'large' vacuole definition, varying from more than 25% (Boitrelle et al, 2011) or 50% of sperm head area (Franco et al, 2008Mauri et al, 2010;Oliveira et al, 2010;Akl et al, 2011;Silva et al, 2012) to a diameter of more than 1.5 lm and visible at 4009 magnification (Watanabe et al, 2011), this cut-off has been proposed to define abnormal sperm vacuole area, using a strict objective MSOME analysis (Saïdi et al, 2008;Perdrix et al, 2012); this value represented the smallest mean vacuole area observed only in abnormal semen samples. Isolation of spermatozoa with large vacuoles was performed using spermatozoa of selected infertile patients presenting semen alterations, whereas normal Table 1 Conventional semen parameters, MSOME parameters and aneuploidy rates in MSOME selected spermatozoa considered as normal or with large vacuoles, in 10 patients and ten controls.…”

Section: Discussionmentioning

confidence: 99%

Modification of chromosomal architecture in human spermatozoa with large vacuoles

Perdrix

Travers

Clatot³

et al. 2012

Andrology

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SUMMARYHuman normal spermatozoa present a specific chromatin organization, illustrated particularly by the non-random chromosome positioning. Spermatozoa with large vacuoles, described using motile sperm organelle morphology organization (MSOME), are associated with nuclear alterations, such as abnormal chromatin condensation and aneuploidy. To question a probable association between large nuclear vacuoles and chromatin disorganization, we evaluated chromosomes X, Y and 18 topography in normal spermatozoa (NS) compared with spermatozoa with large vacuoles (SLV). After centrifugation on a gradient density system, 229 NS (spermatozoa presenting a normal nuclear shape and a vacuole area <6.5% of head area) from 10 normal semen samples and 221 SLV (spermatozoa presenting a vacuole area >13% of head area) from 10 semen samples with teratozoospermia were selected using MSOME. A three-colour FISH was carried out using a-satellite centromeric probes for chromosomes X, Y and 18. For each chromosome, longitudinal and spatial positioning of centromeres was analysed. Distribution of each chromosome was non-random in NS and in SLV, whatever the methodology used. Using longitudinal positioning, distribution of chromosome 18 and chromosome Y centromeres did not differ significantly between SLV and NS. On the contrary, chromosome X centromeres were more frequently positioned in the posterior region of sperm nucleus in SLV (p = 0.01). Considering spatial positioning, distributions differed significantly between SN and SLV for chromosome Y (p = 0.02) and chromosome 18 (p < 10 À4) and marginally for chromosome X (p = 0.08). Our study concluded to a modification in chromosomes X, Y and 18 centromere topography between NS and SLV, representing a novel and supplementary evidence to argue chromatin disorganization in SLV.

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Section: Discussionmentioning

confidence: 99%

Modification of chromosomal architecture in human spermatozoa with large vacuoles

Perdrix

Travers

Clatot³

et al. 2012

Andrology

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“…For the analysis of breast images, Ji and Kim [ 6 ] proposed to use more contextual information for breast image classification, using a convolutional neural network trained on the ICAR2018 dataset to extract tissue features and classify breast cancers into benign, in situ, and invasive breast cancers. In this paper, support vector machines are used to classify the extracted features, but large-scale breast cancer data are lacking.…”

Section: Related Workmentioning

confidence: 99%

B-Ultrasound Image Analysis of Intrauterine Pregnancy Residues after Mid-Term Pregnancy Based on Smart Medical Big Data

Liu

Zhou

et al. 2022

Journal of Healthcare Engineering

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Abdominal B-ultrasound images of intrauterine pregnancy tissue residues were analyzed to discuss their diagnostic value. With the rapid development of computer technology and medical imaging technology, doctors are also faced with more and more medical image diagnosis tasks, and computer-aided diagnosis systems are especially important in order to reduce the work pressure of doctors. In recent years, deep learning has made rapid development and achieved great breakthroughs in various fields. In medical-aided diagnostic systems, deep learning has greatly improved the diagnostic efficiency, but there are no mature research results for abdominal B-ultrasound image recognition of intrauterine pregnancy tissue residues. Therefore, the study of liver ultrasound image classification based on deep learning has important practical application value. In this paper, we propose to give a CNN model optimization method based on grid search. Compared with the conventional CNN model design, this method saves time and effort by eliminating the need to manually adjust parameters based on experience and has an accuracy of more than 92% in classifying abdominal B-ultrasound images of intrauterine pregnancy tissue residues. The diagnosis of intrauterine pregnancy tissue residues by abdominal B-ultrasound can effectively improve the diagnosis and provide important reference for patients to receive treatment, which has high diagnostic value.

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“…According to the literature, pregnancy rates after ICSI (Bartoov et al, 2002;Antinori et al, 2008) and IUI (Akl et al, 2011) depend on the proportion of grade I spermatozoa. In the study by Akl et al (2011), patients with higher pregnancy rates had 2.5% of grade I sperm, whereas patients with low pregnancy rates presented about 1%. Even though we found here that there were few grade I/II spermatozoa after freezing-thawing, it may be of value to evaluate thawed sperm before ART.…”

Section: Morrismentioning

confidence: 99%

Cryopreservation of Human Spermatozoa Decreases the Number of Motile Normal Spermatozoa, Induces Nuclear Vacuolization and Chromatin Decondensation

Boitrelle¹,

Albert²,

Theillac³

et al. 2012

Journal of Andrology

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Even though cryopreservation of human spermatozoa is known to alter sperm motility and viability, it may also induce nuclear damages. The present study set out to determine whether or not cryopreservation alters motile sperm morphology under high magnification and/or is associated with chromatin decondensation. For 25 infertile men, we used high-magnification microscopy to determine the proportions of various types of motile spermatozoa before and after freezing-thawing: morphometrically normal spermatozoa with no vacuole (grade I), #2 small vacuoles (grade II), at least 1 large vacuole or .2 small vacuoles (grade III), and morphometrically abnormal spermatozoa (grade IV). The spermatozoa's chromatin condensation and viability were also assessed before and after freezing-thawing. Cryopreservation induced sperm nuclear vacuolization. It decreased the proportion of grade I + II spermatozoa (P , .001). It induced a decrease in the sperm viability rate (P , .001) and increased the proportion of sperm with noncondensed chromatin (P , .001). The latter parameter was strongly correlated with sperm viability (r 5 0.71; P , .001). However, even motile sperm presented a failure of chromatin condensation after freezingthawing, because the proportion of sperm with noncondensed chromatin was correlated with high-magnification morphology (r 5 20.49 and 0.49 for the proportions of grade I + II and grades III + IV, respectively; P , .001). Cryopreservation alters the organelle morphology of motile human spermatozoa and induces sperm chromatin decondensation. High-magnification microscopy may be useful for evaluating frozen-thawed spermatozoa before use in assisted reproductive technology procedures (such as intrauterine insemination, in vitro fertilization, and intracytoplasmic sperm injection) and for performing research on cryopreservation methods. If frozen-thawed sperm is to be used for intracytoplasmic sperm injection, morphological selection under high magnification may be of particular value.

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Efficacy of motile sperm organelle morphology examination (MSOME) for predicting pregnancy after intrauterine insemination

Cited by 4 publications

References 47 publications

Modification of chromosomal architecture in human spermatozoa with large vacuoles

Modification of chromosomal architecture in human spermatozoa with large vacuoles

B-Ultrasound Image Analysis of Intrauterine Pregnancy Residues after Mid-Term Pregnancy Based on Smart Medical Big Data

Cryopreservation of Human Spermatozoa Decreases the Number of Motile Normal Spermatozoa, Induces Nuclear Vacuolization and Chromatin Decondensation

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