Efficacy of low‐molecular weight chitosan against Candida albicans biofilm on polymethyl methacrylate resin

Abstract

Background Candida biofilm is a major cause of denture stomatitis. We aimed to compare the efficacy of low‐molecular‐weight chitosan solutions against Candida albicans biofilm on polymethyl methacrylate (PMMA) resin. Methods Various types of chitosan were tested for anti‐Candida activity by broth dilution. Two types were selected for further testing on 24‐hour C.albicans biofilm formed on PMMA specimens. Specimens were randomly distributed among experimental groups, including 0.1% and 0.2% acetic acid, 3 and 6 mg/mL of oligomer chitosan and 30 kDa chitosan solutions, effervescent tablet (Polident), and 0.2% chlorhexidine, and immersed for 5 min to 12 h. The viability of C. albicans after cleansing were determined by 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide (MTT) assays. Remaining viability was calculated into percentage relative to respective controls and analyzed using ANOVA with Tukey post‐hoc tests. Live/dead fluorescence microscopy was also performed. Results Chitosan solutions had high efficacy against C. albicans biofilm on PMMA. The mean relative viability compared to control after 12‐h immersion was 6.60 ± 4.75% and 12.72 ± 6.96% for 3 and 6 mg/mL oligomer, respectively, and 11.68 ± 4.81% and 18.08 ± 6.20% for 3 and 6 mg/mL 30 kDa chitosan, respectively. Conclusions Low‐molecular‐weight chitosan solution is an effective antifungal denture cleanser that can significantly reduce C. albicans viability in biofilm on PMMA.

Oral health status and dental treatment needs of psoriatic patients with different therapy regimes

“…It is advantageous to administer a combination of antifungal agent and antimicrobial photodynamic therapy in the management of infection related to Candida biofilm 30,31 . Moreover, low‐molecular‐weight chitosan solution was found as an effective antifungal denture cleanser that can significantly reduce C. albicans viability in biofilms on polymethyl methacrylate resin used for fabrication of acrylic dentures 32 …”

Efficacy of Chitosan Paste as Intracanal Medication Against Enterococcus Faecalis and Candida Albicans Biofilm Compared with Calcium Hydroxide in an In Vitro Root Canal Infection Model

“…However, it needed a long contact time of over 10 min, so it is likely more effective when applied as a root-canal medication rather than as an irrigant. In addition, we also showed that these chitosan derivatives has a minimum fungicidal concentration against C. albicans at 4 mg/ml (17). Thus, in this study, we formulated 4 mg/ml of 1700 KDa chitosan in 0.2% acetic acid into a paste for application as an antimicrobial intracanal medication.…”

Efficacy of chitosan paste as intracanal medication against Enterococcus faecalis and Candida albicans biofilm compared with calcium hydroxide in an in vitro root canal infection model

“…However, it needed a long contact time of over 10 min, so it is likely more effective when applied as a root-canal medication rather than as an irrigant. In addition, we also showed that these chitosan derivatives has a minimum fungicidal concentration against C. albicans at 4 mg/ml [ 16 ]. Thus, in this study, we formulated 4 mg/ml of 1700 KDa chitosan in 0.2% acetic acid into a paste for application as an antimicrobial intracanal medication.…”