Efficacy of low doses of amphotericin B plus allicin against experimental visceral leishmaniasis

Corral, María Jesús; Serrano, Dolores R.; Moreno, Inmaculada; Torrado, J. J.; Domínguez, Mercedes; Alunda, José María

doi:10.1093/jac/dku290

Cited by 24 publications

(35 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Sixty days after infection, L. infantum- infected hamsters were divided into four groups: group 1 was injected with 1.0 mg of UA per kg of body weight (mg/kg); group 2 was injected with 2.0 mg/kg of UA; group 3 was injected with 5.0 mg/kg of amphotericin B (Corral et al., 2014), group 4 was injected with PBS plus 1% DMSO solution (infected control group). Group 5 was constituted by non-infected animals that received only the vehicle solution (PBS control).…”

Section: Methodsmentioning

confidence: 99%

Therapeutic effect of ursolic acid in experimental visceral leishmaniasis

Jesus

Fragoso

Yamamoto

et al. 2017

International Journal for Parasitology: Drugs and Drug Resistan

View full text Add to dashboard Cite

Leishmaniasis is an important neglected tropical disease, affecting more than 12 million people worldwide. The available treatments are not well tolerated and present diverse side effects in patients, justifying the search for new therapeutic compounds. In the present study, the therapeutic potential and toxicity of ursolic acid (UA), isolated from the leaves of Baccharis uncinella C. DC. (Asteraceae), were evaluated in experimental visceral leishmaniasis. To evaluate the therapeutic potential of UA, hamsters infected with L. (L.) infantum were treated daily during 15 days with 1.0 or 2.0 mg UA/kg body weight, or with 5.0 mg amphotericin B/kg body weight by intraperitoneal route. Fifteen days after the last dose, the parasitism of the spleen and liver was stimated and the main histopathological alterations were recorded. The proliferation of splenic mononuclear cells was evaluated and IFN-γ, IL-4, and IL-10 gene expressions were analyzed in spleen fragments. The toxicity of UA and amphotericin B were evaluated in healthy golden hamsters by histological analysis and biochemical parameters. Animals treated with UA had less parasites in the spleen and liver when compared with the infected control group, and they also showed preservation of white and red pulps, which correlate with a high rate of proliferation of splenic mononuclear cells, IFN-γ mRNA and iNOS production. Moreover, animals treated with UA did not present alterations in the levels of AST, ALT, creatinine and urea. Taken together, these findings indicate that UA is an interesting natural compound that should be considered for the development of prototype drugs against visceral leishmaniasis.

show abstract

Section: Methodsmentioning

confidence: 99%

Therapeutic effect of ursolic acid in experimental visceral leishmaniasis

Jesus

Fragoso

Yamamoto

et al. 2017

International Journal for Parasitology: Drugs and Drug Resistan

View full text Add to dashboard Cite

show abstract

“…Hamster infections with Leishmania species causing VL, L.donovani and L.infantum, can be initiated with both promastigotes and amastigotes using different inoculation methods, mainly IC [13,17,[19][20][21][22]30] and IP [17][18][19]30]. Natural vector-transmitted infection has been considered a good method for immunological and pathogenicity studies [15] although requires the availability of sand flies colonies.…”

Section: Discussionmentioning

confidence: 99%

Efficient infection of hamster with Leishmania donovani by retro-orbital inoculation

et al. 2019

Self Cite

View full text Add to dashboard Cite

“…UA was solubilized in DMSO and further PBS (never exceeding 1% DMSO); and AmB was solubilized in sterile water. The highest doses of 1.0 and 5.0 mg/kg from AmB and UA, respectively, were selected based on previously published works [ 21 , 45 , 46 ].…”

Section: Methodsmentioning

confidence: 99%

Ursolic Acid Potentializes Conventional Therapy in Experimental Leishmaniasis

et al. 2020

View full text Add to dashboard Cite

Ursolic acid (UA) is a triterpene with a broad array of pharmacological activities. In leishmaniasis, UA killed different species of parasites, and it was active in the experimental model of cutaneous and visceral leishmaniasis. Thus, the objective of this work was to study the therapeutic efficacy of the conventional drugs amphotericin B (AmB) or glucantime (Glu) combined with UA in experimental visceral and cutaneous leishmaniasis, respectively. L. (L.) infantum-infected hamsters were treated with AmB alone or combined with UA. L. (L.) amazonensis-infected BALB/c mice were treated with Glu alone or combined with UA. Animals were treated for 15 consecutive days by intraperitoneal or intralesional routes. Following one week after the last dose, the tissue parasitism and cellular immune responses were analyzed. Hamsters treated with 0.2 and 1.0 mg/kg of AmB plus 1.0 mg/kg of UA showed low hepatic and splenic parasitisms; however, AmB given as monotherapy did not reduce the number of viable parasites in the spleen of treated animals. In cutaneous leishmaniasis, Glu given as monotherapy was inactive at 2.0 mg/kg, showed mild activity at 10.0 mg/kg, and at 50.0 mg/kg was highly active at eliminating parasites in the skin. When animals were treated with Glu plus UA, higher leishmanicidal activity was observed in comparison to all groups treated with monotherapy schemes, and such activity was related to lesion improvement and upregulation of IFN-γ production. Altogether, data suggest that the association of drugs for the treatment of leishmaniasis can increase the efficiency of the treatment and decrease the toxicity associated to the conventional drugs.

show abstract

Efficacy of low doses of amphotericin B plus allicin against experimental visceral leishmaniasis

Cited by 24 publications

References 36 publications

Therapeutic effect of ursolic acid in experimental visceral leishmaniasis

Therapeutic effect of ursolic acid in experimental visceral leishmaniasis

Efficient infection of hamster with Leishmania donovani by retro-orbital inoculation

Ursolic Acid Potentializes Conventional Therapy in Experimental Leishmaniasis

Contact Info

Product

Resources

About