Efficacy of live attenuated vaccine derived from the Streptococcus agalactiae on the immune responses of Oreochromis niloticus

Laith, A.A.; Abdullah, Mohd Azmuddin; Ibrahim, Wan Nurhafizah Wan; Hussein, Heba A.; Aya, J.; Effendy, A.W.M.; Musa, Najiah

doi:10.1016/j.fsi.2019.04.052

Cited by 24 publications

(19 citation statements)

References 55 publications

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“…A total of 100 μL of serially diluted S. agalactiae were plated onto BHI plates for calculating the number of colony‐forming unit (CFU) (Xu et al., 2019). To determinate the LD50 (Laith et al., 2019), 50 Nile tilapia were divided into 5 groups, with 10 fish per group, and intra‐peritoneally injected with 0.1 ml of increasing concentrations of S. agalactiae (5 × 10 7 ; 1 × 10 8 ; 5.0 × 10 8 ; 1 × 10 9 ; 5 × 10 9 CFU/ml). The control group was injected with 0.1 ml of PBS.…”

Section: Methodsmentioning

confidence: 99%

Molecular cloning, expressional analyses and functional identification of TRAIL in tilapia, Oreochromis niloticus

Sun

et al. 2022

Journal of Fish Diseases

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The tumour necrosis factor superfamily (TNFSF) plays critical roles in tumour apoptosis, tissue morphogenesis and lineage determination. TNFSF10 (TRAIL or Apol-2) belongs to the tumour necrosis factor (TNF) cytokine family and induces rapid apoptosis in a wide variety of tumour cell lines upon binding to death-inducing signalling receptors. In this study, we identified TNFSF10 from Nile tilapia (Oreochromis niloticus) and found it was most closely related to Japanese pufferfish (Takifugu rubripes) TNFSF10. Amino acid identity between tilapia TNFSF10 and mandarin fish (Siniperca chuatsi) TRAIL was 69.2%. The highest expression of TNFSF10 mRNA was observed in the liver. In vitro studies showed that the mRNA expression of TNFSF10 was significantly stimulated by LPS in head kidney leucocytes, but remarkably inhibited by Poly I:C in spleen leucocytes. In vivo studies showed Streptococcus agalactiae infection significantly induced the mRNA expression of TNFSF10 in both the head kidney and spleen. The soluble recombinant protein Trx-TNFSF10 could induce cytotoxicity and apoptosis in HeLa cells with cycloheximide as a promoter. Taken together, these results in this study indicate that TNFSF10 may play important roles in the immune system of Nile tilapia.

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Section: Methodsmentioning

confidence: 99%

Molecular cloning, expressional analyses and functional identification of TRAIL in tilapia, Oreochromis niloticus

Sun

et al. 2022

Journal of Fish Diseases

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“…[57,58,59] in channel catfish ( Ictalurus punctatus ) and Nile tilapia ( Oreochromis niloticus ) or carp species [60], E. tarda in channel catfish and Japanese flounder [61,62], and V. anguillarum in Japanese flounder [63]. Other chemical agents, like acriflavine dye and novobiocin, were used to attenuate Streptococcus agalactiae , S. iniae, E. ictaluri, and A. hydrophila [64,65], but, to date, these vaccines have not been commercialized.…”

Section: Conventional Fish Vaccinesmentioning

confidence: 99%

A Review of Fish Vaccine Development Strategies: Conventional Methods and Modern Biotechnological Approaches

et al. 2019

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Fish immunization has been carried out for over 50 years and is generally accepted as an effective method for preventing a wide range of bacterial and viral diseases. Vaccination efforts contribute to environmental, social, and economic sustainability in global aquaculture. Most licensed fish vaccines have traditionally been inactivated microorganisms that were formulated with adjuvants and delivered through immersion or injection routes. Live vaccines are more efficacious, as they mimic natural pathogen infection and generate a strong antibody response, thus having a greater potential to be administered via oral or immersion routes. Modern vaccine technology has targeted specific pathogen components, and vaccines developed using such approaches may include subunit, or recombinant, DNA/RNA particle vaccines. These advanced technologies have been developed globally and appear to induce greater levels of immunity than traditional fish vaccines. Advanced technologies have shown great promise for the future of aquaculture vaccines and will provide health benefits and enhanced economic potential for producers. This review describes the use of conventional aquaculture vaccines and provides an overview of current molecular approaches and strategies that are promising for new aquaculture vaccine development.

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“…Hence, it is now realized that these bacteria pose a considerable threat to tilapia farming, so producing an effective vaccine is rather urgent. In Malaysia, several types of vaccines, such as inactivated, recombinant, and live attenuated vaccines; Different routes of vaccination, such as oral, injectable, or immersion, were extensively explored by local researchers against S. agalactiae and S. iniae (Table -4) [41][42][43][44][45][46][47][48][49][50][51][52][53][54][55][56][57][58][59].…”

Section: Streptococcosis Vaccinementioning

confidence: 99%

“…Furthermore, continuous effort to develop an efficacious vaccine against streptococcosis has led the local researcher to investigate the potential application of a live-attenuated vaccine, another modern type of vaccine whereby the bacteria hold weakened or less virulent forms. Laith et al [49] previously demonstrated that repeated 187 laboratory passage and chemical attenuation using acriflavine dye could weaken S. agalactiae. Primary oral administration of live-attenuated vaccine and booster at week 4 resulted in RPS of 82% after i.p challenge with a median lethal dose (LD 50 ) of S. agalactiae.…”

Section: Intra-peritoneal Lab Trialmentioning

confidence: 99%

Current status and advances of fish vaccines in Malaysia

Ridzuan

Abdullah²,

Ramly³

et al. 2022

Vet World

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Fish diseases have a significant negative influence on the Malaysian aquaculture industry. Since the 1980s, the sector has grown in size, which has resulted in a rise in the prevalence of infectious outbreaks affecting both freshwater and marine cultured fish species. Demand for commercially available fish vaccinations is predicted to increase as infectious disease outbreaks continue to occur. In Malaysia, aquaculture vaccine research and development (R&D) are still in its infancy, with most efforts concentrating on producing vaccines against bacterial infections, most notably streptococcosis, vibriosis, and motile Aeromonas septicemia. Despite several attempts, no homegrown vaccine has been effectively introduced into the manufacturing pipeline to date. At the moment, only three imported aquatic vaccines have received full permission, a far cry from the 314 and 60 vaccines licensed in the poultry and porcine industries, respectively. This review will describe recent findings regarding the development of aquaculture vaccines for certain fish species and diseases in Malaysia. In our opinion, R&D on fish vaccines is critical to the aquaculture industry's viability.

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Efficacy of live attenuated vaccine derived from the Streptococcus agalactiae on the immune responses of Oreochromis niloticus

Cited by 24 publications

References 55 publications

Molecular cloning, expressional analyses and functional identification of TRAIL in tilapia, Oreochromis niloticus

Molecular cloning, expressional analyses and functional identification of TRAIL in tilapia, Oreochromis niloticus

A Review of Fish Vaccine Development Strategies: Conventional Methods and Modern Biotechnological Approaches

Current status and advances of fish vaccines in Malaysia

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