Efficacy of ivermectin against colon cancer induced by dimethylhydrazine in male wistar rats

Alghamdi, Hamzah A.; Al-Zharani, Mohammed; Aljarba, Nada H.; Alghamdi, Abdullah A. A.; Alghamdi, Abdulrahman A.; Aldahmash, Badr Abdullah; Elnagar, Doaa M.; Alkahtani, Saad

doi:10.1016/j.jsps.2022.06.024

Cited by 2 publications

(4 citation statements)

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“…The expression of human HSPB6 was downregulated in CC tissues compared to its adjacent NC tissues, and the reduction in its expression was around 60% (the average HSBP6 expression in NC tissues was 8.4 ± 2.3, while in CC tissues it was 3.5 ± 1.3) ( Figure 2 ). Previously, we extracted NC treated with saline- and DMH-induced CC from a male Wistar rat [ 31 ]. Thus, before we investigated Hspb6 expression, the proliferation marker Ccnd1 was examined [ 38 ], which was significantly elevated in CC, as well as the differentiation marker Ndrg2 [ 39 ], which was decreased in CC.…”

Section: Resultsmentioning

confidence: 99%

“…Our findings indicated that HSPB6 expression was depleted in CC and in the DMH-induced colon in vivo study [ 31 ], suggesting the possible role of HSPB6 in enhancing tumorigenesis [ 21 , 44 ]. This is in accordance with a report revealed that restoring its expression in HCT116 cells resulted in an induction of the apoptosis pathway [ 21 ].…”

Section: Discussionmentioning

confidence: 98%

“…They were confirmed at King Khalid University Hospital by a team of surgeons and pathologists for their eligibility to enroll in this study. Colon tissues from male Wistar rats were also taken from the normal control group and the DMH-induced group [ 31 ]. All tissues were immediately placed in Eppendorf tubes containing RNAlater [ 14 , 31 ].…”

Section: Methodsmentioning

confidence: 99%

“…Colon tissues from male Wistar rats were also taken from the normal control group and the DMH-induced group [ 31 ]. All tissues were immediately placed in Eppendorf tubes containing RNAlater [ 14 , 31 ].…”

Section: Methodsmentioning

confidence: 99%

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HSPB6 Is Depleted in Colon Cancer Patients and Its Expression Is Induced by 5-aza-2′-Deoxycytidine In Vitro

Almutairi

Alrefaei

et al. 2023

Medicina

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Background and Objectives: Colon cancer (CC) is the second most common cancer in Saudi Arabia, and the number of new cases is expected to increase by 40% by 2040. Sixty percent of patients with CC are diagnosed in the late stage, causing a reduced survival rate. Thus, identifying a new biomarker could contribute to diagnosing CC in the early stages, leading to delivering better therapy and increasing the survival rate. Materials and Methods: HSPB6 expression was investigated in extracted RNA taken from 10 patients with CC and their adjacent normal tissues, as well as in DMH-induced CC and a colon treated with saline taken from a male Wistar rat. Additionally, the DNA of the LoVo and Caco-2 cell lines was collected, and bisulfite was converted to measure the DNA methylation level. This was followed by applying 5-aza-2′-deoxycytidine (AZA) to the LoVo and Caco-2 cell lines for 72 h to see the effect of DNA methylation on HSPB6 expression. Finally, the GeneMANIA database was used to find the interacted genes at transcriptional and translational levels with HSPB6. Results: We found that the expression of HSPB6 was downregulated in 10 CC tissues compared to their adjacent normal colon tissues, as well as in the in vivo study, where its expression was lower in the colon treated with the DMH agent compared to the colon treated with saline. This suggests the possible role of HSPB6 in tumor progression. Moreover, HSPB6 was methylated in two CC cell lines (LoVo and Caco-2), and demethylation with AZA elevated its expression, implying a mechanistic association between DNA methylation and HSPB6 expression. Conclusions: Our findings indicate that HSPB6 is adversely expressed with tumor progression, and its expression may be controlled by DNA methylation. Thus, HSPB6 could be a good biomarker employed in the CC diagnostic process.

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Section: Resultsmentioning

confidence: 99%