Efficacy of ionic liquids on the growth and simultaneous xylanase production by Sporotrichum thermophile: membrane integrity, composition and morphological investigation

Sadaf, Ayesha; Khare, Sunil Kumar

doi:10.1039/c6ra27979a

Cited by 10 publications

(3 citation statements)

References 51 publications

(35 reference statements)

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“…S. thermopile growth and xylanase production in the presence of IL was previously studied in Emerson’s medium (Sadaf and Khare 2017). …”

Section: Methodsmentioning

confidence: 99%

“…Myceliophthora thermophile ) is known to produce a number of industrially relevant enzymes such as xylanases, cellulases, phytases, esterases and pectinases (Singh 2016). To further explore the usefulness of using S. thermophile cells for IL-mediated saccharification, their stability in ILs has been previously studied (Sadaf and Khare 2017). In this work, it was observed that S. thermophile was able to grow profusely and produce xylanase in the presence of two most commonly used ILs, [EMIM][OAc] and [BMIM][MeSO 4 ], indicating high tolerance of the fungus towards these ILs.…”

Section: Introductionmentioning

confidence: 99%

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Proteomic profiling of Sporotrichum thermophile under the effect of ionic liquids: manifestation of an oxidative stress response

2019

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Sporotrichum thermophile, a known producer of industrial enzymes exhibited stability in the presence of ionic liquids (ILs).The study reports, for the first time, the stress response of S. thermophile upon exposure to ILs. In vitro assay showed increased anti-oxidative enzyme levels indicating ROS-mediated oxidative stress by ILs. The proteomic profile and identification of differential proteins confirmed the fungal adaptations by (i) increased expression of glycolytic enzymes and ATP synthases (ii) downregulation of TCA cycle and protein synthesis machinery components (iii) expression of HSP70 and catalase/peroxidase. These changes are indicative of metabolic regulation of many important pathways and how ILs can be used to manipulate protein behavior. Electronic supplementary material The online version of this article (10.1007/s13205-019-1771-z) contains supplementary material, which is available to authorized users.

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“…S. thermopile growth and xylanase production in the presence of IL was previously studied in Emerson’s medium (Sadaf and Khare 2017). …”

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Proteomic profiling of Sporotrichum thermophile under the effect of ionic liquids: manifestation of an oxidative stress response

2019

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“…[ 8,9 ] Recently, our group observed that the increasing trend of lipid accumulation with the application of ionic liquids (ILs). [ 10 ] With the advancement in proteomic and genomic analysis, it has been found that due to stress application, the metabolic flux shifted towards the accumulation of more lipid in the microbes. [ 11 ] ILs with their industrially supportive properties are extensively used as green solvents, however, their compatibility with microbes limits their use.…”

Section: Introductionmentioning

confidence: 99%

Environment‐Friendly Synergistic Abiotic Stress for Enhancing the Yield of Lipids from Oleaginous Yeasts

Chaturvedi

Sadaf

Bhattacharya

et al. 2021

Euro J Lipid Sci & Tech

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The microbial lipids isolated from oleaginous yeasts are a potential alternative to tree borne oils. There is a need to optimize and enhance the production of lipid by various stress approaches. In the present study, yeasts are subjected to physico‐chemical stresses, and growth, as well as lipid concentration at different time intervals are monitored. It is found that the nanoparticles (NP) such as Ag‐NP and Zn‐NP have an inhibitory effect on yeast growth. Most yeast strains show an increase in growth and lipid accumulation when ionic liquid (1‐ethyl‐3‐methylimidazolium acetate) ([EMIM][OAc]) and table salt (NaCl) stress are applied. Lipid is chemically characterized using gas chromatography furnished with flame ionization detector (GC‐FID), GC/MS, and NMR techniques. It contains a higher percentage of saturated fatty acids (SFA: 74.3%), monounsaturated fatty acids (19.1%) with low amounts of polyunsaturated fatty acids (1.9%). The thermo‐stability study reveals that the lipid have higher volatility (380–410 °C) as closely compared with coconut oil, and much lower with respect to the winged bean oil (430–470 °C). The melting point of the lipids (37 °C) is determined through differential scanning calorimetry (DSC). The DSC and physico‐chemical properties are supported that the yeast lipids may use as a cocoa‐butter substitute. Production of lipid under NaCl stress (200 × 10−3 m) is more than 60.4% higher as compared to the control. However, the combined stress effect of NaCl (200 × 10−3 m) and 15 × 10−3 m of [EMIM][OAc] results in more than 96.4% yield of lipid. The exchange of inorganic and organic ions in combined treatment forces the microbial cells to accumulate more amounts of lipid, which may form a lipid‐emulsion layer to protect the cell from osmosis. It is interestingly observed that the stress cells shift the flux to accumulate a significantly improved percentage of SFA, which could be provided better protection cover due to its expanded structure, less reactive characteristics, and completely insoluble nature in ionic‐aqueous solvent. Practical applications: Oleaginous yeast is multiplied in a very limited space, and easily scalable for sustainable production of lipid to meet its commercial demand. This novel approach for enhancing the yield of lipid with the application of synergistic stress in between NaCl and the green solvent (ionic liquid) is being reported for the first time. This lipid has potential alternative applications as cocoa‐butter.

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Thermozymes: Adaptive strategies and tools for their biotechnological applications

Kumar

Dangi

Shukla

et al. 2019

Bioresource Technology

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Efficacy of ionic liquids on the growth and simultaneous xylanase production by Sporotrichum thermophile: membrane integrity, composition and morphological investigation

Cited by 10 publications

References 51 publications

Proteomic profiling of Sporotrichum thermophile under the effect of ionic liquids: manifestation of an oxidative stress response

Proteomic profiling of Sporotrichum thermophile under the effect of ionic liquids: manifestation of an oxidative stress response

Environment‐Friendly Synergistic Abiotic Stress for Enhancing the Yield of Lipids from Oleaginous Yeasts

Thermozymes: Adaptive strategies and tools for their biotechnological applications

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